##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 TimeFormat Endpoint Absorbance Raw FALSE 1 1 1 12 96 1 8 Temperature(¡C) A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 25 0.1127 0.1077 0.1131 0.1146 0.1053 0.0062 0.0916 0.092 0.0853 0.0898 0.0861 0.1032 0.1019 0.0934 0.1048 0.0975 0.0938 0.0055 0.0452 0.0438 0.0447 0.0568 0.0516 0.0674 0.1068 0.1065 0.1102 0.1014 0.1059 0.0053 0.1197 0.1019 0.0918 0.0949 0.1019 0.0474 0.1256 0.1235 0.1149 0.1197 0.0054 0.0055 0.1024 0.0995 0.113 0.1052 0.0053 0 0.1231 0.1022 0.111 0.1071 0.0057 0.0061 0.1191 0.1017 0.0796 0.0838 0.0053 0.0053 0.1221 0.1161 0.1215 0.1194 0.0097 0.0058 0.0986 0.1075 0.1107 0.0983 0.0053 0.0053 0.0057 0.0057 0.0059 0.0058 0.0055 0.0055 0.0055 0.0056 0.0058 0.0056 0.0055 0.0056 0.0054 0.0054 0.0058 0.0057 0.0061 0.0053 0.0053 0.0056 0.0055 0.0056 0.0053 0.0057 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 0.1127 0.10770000000000002 0.1131 0.11460000000000001 0.1053 0.0062000000000000006 0.0916 0.092 0.0853 0.0898 0.08610000000000001 0.10320000000000001 0.10190000000000002 0.093400000000000011 0.1048 0.0975 0.093800000000000008 0.0055000000000000014 0.045200000000000004 0.043800000000000006 0.044700000000000004 0.0568 0.051600000000000007 0.0674 0.1068 0.10650000000000001 0.11019999999999999 0.10140000000000002 0.1059 0.0053000000000000026 0.1197 0.10190000000000002 0.0918 0.0949 0.10190000000000002 0.047400000000000012 0.12560000000000002 0.1235 0.1149 0.1197 0.005400000000000002 0.0055000000000000014 0.10240000000000002 0.0995 0.11300000000000002 0.10520000000000002 0.0053000000000000026 0 0.12310000000000002 0.10220000000000001 0.11100000000000002 0.1071 0.0057 0.0061000000000000047 0.11910000000000001 0.10170000000000001 0.0796 0.0838 0.0053000000000000026 0.0053000000000000026 0.12210000000000001 0.11610000000000001 0.1215 0.1194 0.0097000000000000038 0.0058000000000000031 0.098600000000000007 0.10750000000000001 0.11069999999999999 0.0983 0.0053000000000000026 0.0053000000000000026 0.0057 0.0057 0.005899999999999999 0.0058000000000000031 0.0055000000000000014 0.0055000000000000014 0.0055000000000000014 0.0056000000000000008 0.0058000000000000031 0.0056000000000000008 0.0055000000000000014 0.0056000000000000008 0.005400000000000002 0.005400000000000002 0.0058000000000000031 0.0057 0.0061000000000000047 0.0053000000000000026 0.0053000000000000026 0.0056000000000000008 0.0055000000000000014 0.0056000000000000008 0.0053000000000000026 0.0057 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 3.220 3.207 A12 0.103 0.103 0.000 0.0 02 1.610 1.649 B12 0.067 0.067 0.000 0.0 03 0.805 0.779 C12 0.047 0.047 0.000 0.0 Group Summaries MinStd Smallest standard value: 0.047 Min(MeanValue) MaxStd Largest standard value: 0.103 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 0.113 R 3.620 3.620 0.000 0.0 02 A2 0.108 R 3.403 3.403 0.000 0.0 03 A3 0.113 R 3.638 3.638 0.000 0.0 04 A4 0.115 R 3.703 3.703 0.000 0.0 05 A5 0.105 R 3.298 3.298 0.000 0.0 06 B1 0.102 3.150 3.150 0.000 0.0 07 B2 0.093 2.781 2.781 0.000 0.0 08 B3 0.105 R 3.277 3.277 0.000 0.0 09 B4 0.098 2.959 2.959 0.000 0.0 10 B5 0.094 2.798 2.798 0.000 0.0 11 C1 0.107 R 3.364 3.364 0.000 0.0 12 C2 0.107 R 3.351 3.351 0.000 0.0 13 C3 0.110 R 3.512 3.512 0.000 0.0 14 C4 0.101 3.129 3.129 0.000 0.0 15 C5 0.106 R 3.324 3.324 0.000 0.0 16 D1 0.126 R 4.182 4.182 0.000 0.0 17 D2 0.124 R 4.090 4.090 0.000 0.0 18 D3 0.115 R 3.716 3.716 0.000 0.0 19 D4 0.120 R 3.925 3.925 0.000 0.0 20 E1 0.123 R 4.073 4.073 0.000 0.0 21 E2 0.102 3.163 3.163 0.000 0.0 22 E3 0.111 R 3.546 3.546 0.000 0.0 23 E4 0.107 R 3.377 3.377 0.000 0.0 24 F1 0.122 R 4.029 4.029 0.000 0.0 25 F2 0.116 R 3.768 3.768 0.000 0.0 26 F3 0.122 R 4.003 4.003 0.000 0.0 27 F4 0.119 R 3.912 3.912 0.000 0.0 28 A7 0.092 2.702 2.702 0.000 0.0 29 A8 0.092 2.720 2.720 0.000 0.0 30 A9 0.085 2.428 2.428 0.000 0.0 31 A10 0.090 2.624 2.624 0.000 0.0 32 A11 0.086 2.463 2.463 0.000 0.0 33 B7 0.045 R 0.683 0.683 0.000 0.0 34 B8 0.044 R 0.622 0.622 0.000 0.0 35 B9 0.045 R 0.661 0.661 0.000 0.0 36 B10 0.057 1.188 1.188 0.000 0.0 37 B11 0.052 0.962 0.962 0.000 0.0 38 C7 0.120 R 3.925 3.925 0.000 0.0 39 C8 0.102 3.150 3.150 0.000 0.0 40 C9 0.092 2.711 2.711 0.000 0.0 41 C10 0.095 2.846 2.846 0.000 0.0 42 C11 0.102 3.150 3.150 0.000 0.0 43 D7 0.102 3.172 3.172 0.000 0.0 44 D8 0.100 3.046 3.046 0.000 0.0 45 D9 0.113 R 3.633 3.633 0.000 0.0 46 D10 0.105 R 3.294 3.294 0.000 0.0 47 E7 0.119 R 3.899 3.899 0.000 0.0 48 E8 0.102 3.142 3.142 0.000 0.0 49 E9 0.080 2.180 2.180 0.000 0.0 50 E10 0.084 2.363 2.363 0.000 0.0 51 F7 0.099 3.007 3.007 0.000 0.0 52 F8 0.108 R 3.394 3.394 0.000 0.0 53 F9 0.111 R 3.533 3.533 0.000 0.0 54 F10 0.098 2.994 2.994 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20160817_plate results; Date Last Saved: 8/17/2016 12:46:12 PM