##BLOCKS= 5 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 32 76159248 56709620 48143784 1684 1648 1777 1695 1637 1535 1714 1659 1667 26133918 23700078 31444192 1728 1733 1629 1616 1691 1585 1660 1623 1614 8365610 8841120 58280 1740 1660 1711 1665 1645 1698 1593 1613 1558 4354838 4340058 13069978 1709 1648 1604 1674 1586 1692 1673 1609 1657 2034726 2082981 2091271 1717 1704 1686 1525 1562 1794 1535 3097 1719 1008664 1028423 1037032 1651 1775 1775 1632 1663 1692 1802 1577 1685 515007 506044 523727 1626 1507 1584 1999 1568 1575 1676 1681 1621 53395 55508 58487 1613 1521 1600 1528 1654 1523 1687 1596 1623 1 2 3 4 5 6 7 8 9 10 11 12 76159248 56709620 Masked 1684 1648 1777 1695 1637 1535 1714 1659 1667 26133918 23700078 31444192 1728 1733 1629 1616 1691 1585 1660 1623 1614 8365610 8841120 Masked 1740 1660 1711 1665 1645 1698 1593 1613 1558 4354838 4340058 Masked 1709 1648 1604 1674 1586 1692 1673 1609 1657 2034726 2082981 2091271 1717 1704 1686 1525 1562 1794 1535 3097 1719 1008664 1028423 1037032 1651 1775 1775 1632 1663 1692 1802 1577 1685 515007 506044 523727 1626 1507 1584 1999 1568 1575 1676 1681 1621 53395 55508 58487 1613 1521 1600 1528 1654 1523 1687 1596 1623 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 36.5 58211384 61287132 48778428 73976152 60109536 72898768 77995376 81401304 56569100 73572720 80657112 50359844 2439160 7531335 2752391 5522799 4500879 6710357 6000858 7259957 1283642 529321 4950711 7541818 259897 1288763 5077561 7777316 2924754 6315582 3214104 3562615 7376976 826848 6583356 2797478 621030 7796451 1588541 5242770 4788899 1374619 5177317 4998623 3103242 2911689 7085036 6755663 10373649 4633290 10381320 3792563 4988672 5676662 6489901 8675266 9323820 403558 12382346 674737 1619 1470 1521 1543 1569 1462 1563 1504 1512 1558 1640 1627 1503 1477 1564 1626 1590 1571 1538 1522 1544 1522 1559 1522 1582 1575 1516 1510 1484 1652 1543 1368 1644 1587 1603 1528 1 2 3 4 5 6 7 8 9 10 11 12 58211384 61287132 48778428 73976152 60109536 72898768 77995376 81401304 56569100 73572720 80657112 50359844 2439160 7531335 2752391 5522799 4500879 6710357 6000858 7259957 1283642 529321 4950711 7541818 259897 1288763 5077561 7777316 2924754 6315582 3214104 3562615 7376976 826848 6583356 2797478 621030 7796451 1588541 5242770 4788899 1374619 5177317 4998623 3103242 2911689 7085036 6755663 10373649 4633290 10381320 3792563 4988672 5676662 6489901 8675266 9323820 403558 12382346 674737 1619 1470 1521 1543 1569 1462 1563 1504 1512 1558 1640 1627 1503 1477 1564 1626 1590 1571 1538 1522 1544 1522 1559 1522 1582 1575 1516 1510 1484 1652 1543 1368 1644 1587 1603 1528 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 37 17043138 3458518 11936731 10990052 15107317 15295096 1003938 13723060 6452063 9372753 17768394 5340338 61560 78381 95662 63434 9604988 6522095 7658276 4179430 11668667 90524 157778 66487 58616 72346 78253 67981 210378 63911 5769800 70402 55017 6501407 2735562 9517723 2962360 12090679 7906349 14903294 14090897 8157375 6679935 2384177 5937253 11698678 9015888 3714494 9873241 11653815 10031488 12124062 6706566 8405290 2837738 10788339 54608 55174 1876 2623 1515 1436 1515 1461 1640 1544 1563 1633 1605 1625 1450 1588 1541 1547 1523 1652 1652 1536 1625 1621 1662 1492 1813 1546 1441 1607 1511 1600 1607 1528 1648 1644 1489 1566 1461 1564 1 2 3 4 5 6 7 8 9 10 11 12 17043138 3458518 11936731 10990052 15107317 15295096 1003938 13723060 6452063 9372753 17768394 5340338 61560 78381 95662 63434 9604988 6522095 7658276 4179430 11668667 90524 157778 66487 58616 72346 78253 67981 210378 63911 5769800 70402 55017 6501407 2735562 9517723 2962360 12090679 7906349 14903294 14090897 8157375 6679935 2384177 5937253 11698678 9015888 3714494 9873241 11653815 10031488 12124062 6706566 8405290 2837738 10788339 54608 55174 1876 2623 1515 1436 1515 1461 1640 1544 1563 1633 1605 1625 1450 1588 1541 1547 1523 1652 1652 1536 1625 1621 1662 1492 1813 1546 1441 1607 1511 1600 1607 1528 1648 1644 1489 1566 1461 1564 ~End Group: Standards Sample Concentration ng BackCalcConc Wells Value MeanValue SD CV 01 100.000 119.310 A1 76159248.000 66434434.000 13752963.850 20.7 89.850 A2 56709620.000 Masked A3 Masked 02 50.000 43.536 B1 26133918.000 27092729.333 3960090.250 14.6 39.849 B2 23700078.000 51.579 B3 31444192.000 03 25.000 16.622 C1 8365610.000 8603365.000 336236.346 3.9 17.342 C2 8841120.000 Masked C3 Masked 04 12.500 10.547 D1 4354838.000 4347448.000 10451.038 0.2 10.524 D2 4340058.000 Masked D3 Masked 05 6.250 7.032 E1 2034726.000 2069659.333 30535.788 1.5 7.105 E2 2082981.000 7.118 E3 2091271.000 06 3.125 5.478 F1 1008664.000 1024706.333 14544.623 1.4 5.508 F2 1028423.000 5.521 F3 1037032.000 07 1.563 4.730 G1 515007.000 514926.000 8841.778 1.7 4.717 G2 506044.000 4.743 G3 523727.000 08 0.000 4.031 H1 53395.000 55796.667 2558.244 4.6 4.034 H2 55508.000 4.039 H3 58487.000 Group Summaries MinStd Smallest standard value: 55796.667 Min(MeanValue) MaxStd Largest standard value: 66434434.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 58211384.000 92.124 92.124 0.000 0.0 02 A2 61287132.000 96.783 96.783 0.000 0.0 03 A3 48778428.000 77.836 77.836 0.000 0.0 04 A4 73976152.000 R 116.004 116.004 0.000 0.0 05 A5 60109536.000 95.000 95.000 0.000 0.0 06 A6 72898768.000 R 114.372 114.372 0.000 0.0 061 A1 17043138.000 29.766 29.766 0.000 0.0 062 A2 3458518.000 9.189 9.189 0.000 0.0 063 A3 11936731.000 22.031 22.031 0.000 0.0 064 A4 10990052.000 20.597 20.597 0.000 0.0 065 A5 15107317.000 26.834 26.834 0.000 0.0 066 A6 15295096.000 27.118 27.118 0.000 0.0 067 A7 1003938.000 5.471 5.471 0.000 0.0 068 A8 13723060.000 24.737 24.737 0.000 0.0 069 A9 6452063.000 13.723 13.723 0.000 0.0 07 A7 77995376.000 R 122.092 122.092 0.000 0.0 070 A10 9372753.000 18.147 18.147 0.000 0.0 071 A11 17768394.000 30.864 30.864 0.000 0.0 072 A12 5340338.000 12.039 12.039 0.000 0.0 073 B1 61560.000 4.043 4.043 0.000 0.0 074 B2 78381.000 4.069 4.069 0.000 0.0 075 B3 95662.000 4.095 4.095 0.000 0.0 076 B4 63434.000 4.046 4.046 0.000 0.0 077 B5 9604988.000 18.499 18.499 0.000 0.0 078 B6 6522095.000 13.829 13.829 0.000 0.0 079 B7 7658276.000 15.550 15.550 0.000 0.0 08 A8 81401304.000 R 127.251 127.251 0.000 0.0 080 B8 4179430.000 10.281 10.281 0.000 0.0 081 B9 11668667.000 21.625 21.625 0.000 0.0 082 B10 90524.000 4.087 4.087 0.000 0.0 083 B11 157778.000 4.189 4.189 0.000 0.0 084 B12 66487.000 4.051 4.051 0.000 0.0 085 C1 58616.000 4.039 4.039 0.000 0.0 086 C2 72346.000 4.060 4.060 0.000 0.0 087 C3 78253.000 4.069 4.069 0.000 0.0 088 C4 67981.000 4.053 4.053 0.000 0.0 089 C5 210378.000 4.269 4.269 0.000 0.0 09 A9 56569100.000 89.637 89.637 0.000 0.0 090 C6 63911.000 4.047 4.047 0.000 0.0 091 C7 5769800.000 12.690 12.690 0.000 0.0 092 C8 70402.000 4.057 4.057 0.000 0.0 093 C9 55017.000 R 4.033 4.033 0.000 0.0 094 C10 6501407.000 13.798 13.798 0.000 0.0 095 C11 2735562.000 8.094 8.094 0.000 0.0 096 C12 9517723.000 18.367 18.367 0.000 0.0 097 D1 2962360.000 8.437 8.437 0.000 0.0 098 D2 12090679.000 22.264 22.264 0.000 0.0 099 D3 7906349.000 15.926 15.926 0.000 0.0 10 A10 73572720.000 R 115.393 115.393 0.000 0.0 100 D4 14903294.000 26.525 26.525 0.000 0.0 101 D5 14090897.000 25.294 25.294 0.000 0.0 102 D6 8157375.000 16.306 16.306 0.000 0.0 103 D7 6679935.000 14.068 14.068 0.000 0.0 104 D8 2384177.000 7.561 7.561 0.000 0.0 105 D9 5937253.000 12.943 12.943 0.000 0.0 106 D10 11698678.000 21.670 21.670 0.000 0.0 107 D11 9015888.000 17.607 17.607 0.000 0.0 108 D12 3714494.000 9.577 9.577 0.000 0.0 109 E1 9873241.000 18.905 18.905 0.000 0.0 11 A11 80657112.000 R 126.124 126.124 0.000 0.0 110 E2 11653815.000 21.602 21.602 0.000 0.0 111 E3 10031488.000 19.145 19.145 0.000 0.0 112 E4 12124062.000 22.315 22.315 0.000 0.0 113 E5 6706566.000 14.109 14.109 0.000 0.0 114 E6 8405290.000 16.682 16.682 0.000 0.0 115 E7 2837738.000 8.249 8.249 0.000 0.0 116 E8 10788339.000 20.291 20.291 0.000 0.0 12 A12 50359844.000 80.231 80.231 0.000 0.0 13 B1 2439160.000 7.645 7.645 0.000 0.0 14 B2 7531335.000 15.358 15.358 0.000 0.0 15 B3 2752391.000 8.119 8.119 0.000 0.0 16 B4 5522799.000 12.316 12.316 0.000 0.0 17 B5 4500879.000 10.768 10.768 0.000 0.0 18 B6 6710357.000 14.114 14.114 0.000 0.0 19 B7 6000858.000 13.040 13.040 0.000 0.0 20 B8 7259957.000 14.947 14.947 0.000 0.0 21 B9 1283642.000 5.894 5.894 0.000 0.0 22 B10 529321.000 4.752 4.752 0.000 0.0 23 B11 4950711.000 11.449 11.449 0.000 0.0 24 B12 7541818.000 15.374 15.374 0.000 0.0 25 C1 259897.000 4.344 4.344 0.000 0.0 26 C2 1288763.000 5.902 5.902 0.000 0.0 27 C3 5077561.000 11.641 11.641 0.000 0.0 28 C4 7777316.000 15.731 15.731 0.000 0.0 29 C5 2924754.000 8.380 8.380 0.000 0.0 30 C6 6315582.000 13.516 13.516 0.000 0.0 31 C7 3214104.000 8.819 8.819 0.000 0.0 32 C8 3562615.000 9.347 9.347 0.000 0.0 33 C9 7376976.000 15.124 15.124 0.000 0.0 34 C10 826848.000 5.203 5.203 0.000 0.0 35 C11 6583356.000 13.922 13.922 0.000 0.0 36 C12 2797478.000 8.188 8.188 0.000 0.0 37 D1 621030.000 4.891 4.891 0.000 0.0 38 D2 7796451.000 15.760 15.760 0.000 0.0 39 D3 1588541.000 6.356 6.356 0.000 0.0 40 D4 5242770.000 11.891 11.891 0.000 0.0 41 D5 4788899.000 11.204 11.204 0.000 0.0 42 D6 1374619.000 6.032 6.032 0.000 0.0 43 D7 5177317.000 11.792 11.792 0.000 0.0 44 D8 4998623.000 11.522 11.522 0.000 0.0 45 D9 3103242.000 8.651 8.651 0.000 0.0 46 D10 2911689.000 8.361 8.361 0.000 0.0 47 D11 7085036.000 14.682 14.682 0.000 0.0 48 D12 6755663.000 14.183 14.183 0.000 0.0 49 E1 10373649.000 19.663 19.663 0.000 0.0 50 E2 4633290.000 10.968 10.968 0.000 0.0 51 E3 10381320.000 19.675 19.675 0.000 0.0 52 E4 3792563.000 9.695 9.695 0.000 0.0 53 E5 4988672.000 11.507 11.507 0.000 0.0 54 E6 5676662.000 12.549 12.549 0.000 0.0 55 E7 6489901.000 13.781 13.781 0.000 0.0 56 E8 8675266.000 17.091 17.091 0.000 0.0 57 E9 9323820.000 18.073 18.073 0.000 0.0 58 E10 403558.000 4.561 4.561 0.000 0.0 59 E11 12382346.000 22.706 22.706 0.000 0.0 60 E12 674737.000 4.972 4.972 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20170413_Minipreps; Date Last Saved: 4/13/2017 3:38:32 PM