##BLOCKS= 5 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24 22809236 25197980 25441960 1333 1279 1258 1099 1219 1215 1167 1169 1162 12606939 12519093 12983090 1189 1274 1208 1124 1185 1124 1076 1174 1035 6402113 6672128 6663620 1357 1264 1428 1096 1175 1055 1084 1100 1130 3057799 3252135 3201191 1197 1291 1270 1109 1145 1141 1163 973 1014 1527896 1627982 1684141 1283 1267 1374 1199 1151 1124 1024 1088 1197 738800 776190 787576 1174 1242 1287 1180 1019 1178 1189 1160 1112 378757 410074 402719 1232 1323 1359 1087 1087 1186 1109 1102 1096 27406 28113 24733 1211 1233 1288 1057 1054 1145 1175 1238 1070 1 2 3 4 5 6 7 8 9 10 11 12 22809236 25197980 25441960 1333 1279 1258 1099 1219 1215 1167 1169 1162 12606939 12519093 12983090 1189 1274 1208 1124 1185 1124 1076 1174 1035 6402113 6672128 6663620 1357 1264 1428 1096 1175 1055 1084 1100 1130 3057799 3252135 3201191 1197 1291 1270 1109 1145 1141 1163 973 1014 1527896 1627982 1684141 1283 1267 1374 1199 1151 1124 1024 1088 1197 738800 776190 787576 1174 1242 1287 1180 1019 1178 1189 1160 1112 378757 410074 402719 1232 1323 1359 1087 1087 1186 1109 1102 1096 27406 28113 24733 1211 1233 1288 1057 1054 1145 1175 1238 1070 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24 16329656 4694310 8889440 1293525 12374803 8290959 11567607 7632489 9454011 13344289 12737726 19137366 11459918 6649953 10228395 1783418 10012874 8872433 13454025 6813407 9526931 10254874 10196860 14608479 559517 5256279 12369367 1520830 10648321 12879358 11008914 5617624 7053816 10244067 11986472 10466649 387316 6057477 12880431 80701 11855346 9060114 14134051 6019796 8211372 8760478 11083045 10359153 12131758 6519040 18454970 5770485 10118879 13871014 11459573 13026672 652498 2338360 8380970 9060852 710123 6002913 18989172 5974880 13022324 12685717 11174638 9153625 666164 1183662 13427965 10226765 13554301 8709861 23711758 13024253 13029220 13743491 11724260 8056550 795912 4708589 10979906 18622258 11180086 21630896 21979884 16512069 33138526 26912850 12227837 8735915 718925 1605690 11272242 7321901 1 2 3 4 5 6 7 8 9 10 11 12 16329656 4694310 8889440 1293525 12374803 8290959 11567607 7632489 9454011 13344289 12737726 19137366 11459918 6649953 10228395 1783418 10012874 8872433 13454025 6813407 9526931 10254874 10196860 14608479 559517 5256279 12369367 1520830 10648321 12879358 11008914 5617624 7053816 10244067 11986472 10466649 387316 6057477 12880431 80701 11855346 9060114 14134051 6019796 8211372 8760478 11083045 10359153 12131758 6519040 18454970 5770485 10118879 13871014 11459573 13026672 652498 2338360 8380970 9060852 710123 6002913 18989172 5974880 13022324 12685717 11174638 9153625 666164 1183662 13427965 10226765 13554301 8709861 23711758 13024253 13029220 13743491 11724260 8056550 795912 4708589 10979906 18622258 11180086 21630896 21979884 16512069 33138526 26912850 12227837 8735915 718925 1605690 11272242 7321901 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24 8780269 9751280 21936160 9864146 11468748 7255392 2902594 4235257 1184468 4084514 18659980 5559338 13643316 6655641 16667203 9159559 9651407 8854370 2824780 4652320 917394 3794283 18980778 2656657 7265086 4973777 15361177 6353093 11022272 7849472 2545840 7453048 1464622 3658382 15389759 3527895 8583535 6918779 16279223 7572395 10844035 10162268 2447784 8462685 1664829 5203585 12939382 4183748 9045908 6491526 4952281 11897537 8642963 8215475 6541250 7393228 10076163 11056010 48257 71670 6474265 8195893 4468737 11570396 9544044 7872448 8882937 13025940 12238511 38824 26546 29203 5475391 8653282 4183337 10430647 9103535 8448827 5891535 9732961 10097811 7784568 526222 104388 7058257 9789204 2564528 10577807 9048692 9301619 9627828 9415965 13668742 11096431 7996000 47592 1 2 3 4 5 6 7 8 9 10 11 12 8780269 9751280 21936160 9864146 11468748 7255392 2902594 4235257 1184468 4084514 18659980 5559338 13643316 6655641 16667203 9159559 9651407 8854370 2824780 4652320 917394 3794283 18980778 2656657 7265086 4973777 15361177 6353093 11022272 7849472 2545840 7453048 1464622 3658382 15389759 3527895 8583535 6918779 16279223 7572395 10844035 10162268 2447784 8462685 1664829 5203585 12939382 4183748 9045908 6491526 4952281 11897537 8642963 8215475 6541250 7393228 10076163 11056010 48257 71670 6474265 8195893 4468737 11570396 9544044 7872448 8882937 13025940 12238511 38824 26546 29203 5475391 8653282 4183337 10430647 9103535 8448827 5891535 9732961 10097811 7784568 526222 104388 7058257 9789204 2564528 10577807 9048692 9301619 9627828 9415965 13668742 11096431 7996000 47592 ~End Group: Standards Sample Concentration ng BackCalcConc Wells Value MeanValue SD CV 01 100.000 92.318 A1 22809236.000 24483058.667 1454696.979 5.9 102.038 A2 25197980.000 103.031 A3 25441960.000 02 50.000 50.804 B1 12606939.000 12703040.667 246475.053 1.9 50.446 B2 12519093.000 52.334 B3 12983090.000 03 25.000 25.556 C1 6402113.000 6579287.000 153496.144 2.3 26.654 C2 6672128.000 26.620 C3 6663620.000 04 12.500 11.947 D1 3057799.000 3170375.000 100766.262 3.2 12.738 D2 3252135.000 12.531 D3 3201191.000 05 6.250 5.722 E1 1527896.000 1613339.667 79144.952 4.9 6.129 E2 1627982.000 6.358 E3 1684141.000 06 3.125 2.511 F1 738800.000 767522.000 25517.155 3.3 2.663 F2 776190.000 2.709 F3 787576.000 07 1.563 1.046 G1 378757.000 397183.333 16375.937 4.1 1.173 G2 410074.000 1.143 G3 402719.000 08 0.000 -0.384 H1 27406.000 26750.667 1782.750 6.7 -0.381 H2 28113.000 -0.395 H3 24733.000 Group Summaries MinStd Smallest standard value: 26750.667 Min(MeanValue) MaxStd Largest standard value: 24483058.667 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 16329656.000 65.952 65.952 0.000 0.0 02 B1 11459918.000 46.136 46.136 0.000 0.0 03 C1 559517.000 1.781 1.781 0.000 0.0 04 D1 387316.000 1.081 1.081 0.000 0.0 05 E1 12131758.000 48.870 48.870 0.000 0.0 06 F1 710123.000 2.394 2.394 0.000 0.0 07 G1 13554301.000 54.659 54.659 0.000 0.0 08 H1 11180086.000 44.998 44.998 0.000 0.0 09 A2 4694310.000 18.606 18.606 0.000 0.0 097 A1 8780269.000 35.233 35.233 0.000 0.0 098 B1 13643316.000 55.021 55.021 0.000 0.0 099 C1 7265086.000 29.067 29.067 0.000 0.0 10 B2 6649953.000 26.564 26.564 0.000 0.0 100 D1 8583535.000 34.432 34.432 0.000 0.0 101 E1 9045908.000 36.314 36.314 0.000 0.0 102 F1 6474265.000 25.849 25.849 0.000 0.0 103 G1 5475391.000 21.785 21.785 0.000 0.0 104 H1 7058257.000 28.226 28.226 0.000 0.0 105 A2 9751280.000 39.184 39.184 0.000 0.0 106 B2 6655641.000 26.587 26.587 0.000 0.0 107 C2 4973777.000 19.744 19.744 0.000 0.0 108 D2 6918779.000 27.658 27.658 0.000 0.0 109 E2 6491526.000 25.919 25.919 0.000 0.0 11 C2 5256279.000 20.893 20.893 0.000 0.0 110 F2 8195893.000 32.855 32.855 0.000 0.0 111 G2 8653282.000 34.716 34.716 0.000 0.0 112 H2 9789204.000 39.338 39.338 0.000 0.0 113 A3 21936160.000 88.765 88.765 0.000 0.0 114 B3 16667203.000 67.325 67.325 0.000 0.0 115 C3 15361177.000 62.011 62.011 0.000 0.0 116 D3 16279223.000 65.747 65.747 0.000 0.0 117 E3 4952281.000 19.656 19.656 0.000 0.0 118 F3 4468737.000 17.688 17.688 0.000 0.0 119 G3 4183337.000 16.527 16.527 0.000 0.0 12 D2 6057477.000 24.153 24.153 0.000 0.0 120 H3 2564528.000 9.940 9.940 0.000 0.0 121 A4 9864146.000 39.643 39.643 0.000 0.0 122 B4 9159559.000 36.776 36.776 0.000 0.0 123 C4 6353093.000 25.356 25.356 0.000 0.0 124 D4 7572395.000 30.318 30.318 0.000 0.0 125 E4 11897537.000 47.917 47.917 0.000 0.0 126 F4 11570396.000 46.586 46.586 0.000 0.0 127 G4 10430647.000 41.948 41.948 0.000 0.0 128 H4 10577807.000 42.547 42.547 0.000 0.0 129 A5 11468748.000 46.172 46.172 0.000 0.0 13 E2 6519040.000 26.031 26.031 0.000 0.0 130 B5 9651407.000 38.777 38.777 0.000 0.0 131 C5 11022272.000 44.356 44.356 0.000 0.0 132 D5 10844035.000 43.630 43.630 0.000 0.0 133 E5 8642963.000 34.674 34.674 0.000 0.0 134 F5 9544044.000 38.340 38.340 0.000 0.0 135 G5 9103535.000 36.548 36.548 0.000 0.0 136 H5 9048692.000 36.325 36.325 0.000 0.0 137 A6 7255392.000 29.028 29.028 0.000 0.0 138 B6 8854370.000 35.534 35.534 0.000 0.0 139 C6 7849472.000 31.445 31.445 0.000 0.0 14 F2 6002913.000 23.931 23.931 0.000 0.0 140 D6 10162268.000 40.856 40.856 0.000 0.0 141 E6 8215475.000 32.934 32.934 0.000 0.0 142 F6 7872448.000 31.539 31.539 0.000 0.0 143 G6 8448827.000 33.884 33.884 0.000 0.0 144 H6 9301619.000 37.354 37.354 0.000 0.0 145 A7 2902594.000 11.316 11.316 0.000 0.0 146 B7 2824780.000 10.999 10.999 0.000 0.0 147 C7 2545840.000 9.864 9.864 0.000 0.0 148 D7 2447784.000 9.465 9.465 0.000 0.0 149 E7 6541250.000 26.122 26.122 0.000 0.0 15 G2 8709861.000 34.946 34.946 0.000 0.0 150 F7 8882937.000 35.650 35.650 0.000 0.0 151 G7 5891535.000 23.478 23.478 0.000 0.0 152 H7 9627828.000 38.681 38.681 0.000 0.0 153 A8 4235257.000 16.738 16.738 0.000 0.0 154 B8 4652320.000 18.436 18.436 0.000 0.0 155 C8 7453048.000 29.832 29.832 0.000 0.0 156 D8 8462685.000 33.940 33.940 0.000 0.0 157 E8 7393228.000 29.589 29.589 0.000 0.0 158 F8 13025940.000 52.509 52.509 0.000 0.0 159 G8 9732961.000 39.109 39.109 0.000 0.0 16 H2 21630896.000 87.523 87.523 0.000 0.0 160 H8 9415965.000 37.819 37.819 0.000 0.0 161 A9 1184468.000 4.324 4.324 0.000 0.0 162 B9 917394.000 3.238 3.238 0.000 0.0 163 C9 1464622.000 5.464 5.464 0.000 0.0 164 D9 1664829.000 6.279 6.279 0.000 0.0 165 E9 10076163.000 40.506 40.506 0.000 0.0 166 F9 12238511.000 49.305 49.305 0.000 0.0 167 G9 10097811.000 40.594 40.594 0.000 0.0 168 H9 13668742.000 55.124 55.124 0.000 0.0 169 A10 4084514.000 16.125 16.125 0.000 0.0 17 A3 8889440.000 35.677 35.677 0.000 0.0 170 B10 3794283.000 14.944 14.944 0.000 0.0 171 C10 3658382.000 14.391 14.391 0.000 0.0 172 D10 5203585.000 20.679 20.679 0.000 0.0 173 E10 11056010.000 44.493 44.493 0.000 0.0 174 F10 38824.000 -0.337 -0.337 0.000 0.0 175 G10 7784568.000 31.181 31.181 0.000 0.0 176 H10 11096431.000 44.657 44.657 0.000 0.0 177 A11 18659980.000 75.434 75.434 0.000 0.0 178 B11 18980778.000 76.740 76.740 0.000 0.0 179 C11 15389759.000 62.127 62.127 0.000 0.0 18 B3 10228395.000 41.125 41.125 0.000 0.0 180 D11 12939382.000 52.156 52.156 0.000 0.0 181 E11 48257.000 -0.299 -0.299 0.000 0.0 182 F11 26546.000 R -0.387 -0.387 0.000 0.0 183 G11 526222.000 1.646 1.646 0.000 0.0 184 H11 7996000.000 32.041 32.041 0.000 0.0 185 A12 5559338.000 22.126 22.126 0.000 0.0 186 B12 2656657.000 10.315 10.315 0.000 0.0 187 C12 3527895.000 13.860 13.860 0.000 0.0 188 D12 4183748.000 16.529 16.529 0.000 0.0 189 E12 71670.000 -0.204 -0.204 0.000 0.0 19 C3 12369367.000 49.837 49.837 0.000 0.0 190 F12 29203.000 -0.376 -0.376 0.000 0.0 191 G12 104388.000 -0.071 -0.071 0.000 0.0 192 H12 47592.000 -0.302 -0.302 0.000 0.0 20 D3 12880431.000 51.917 51.917 0.000 0.0 21 E3 18454970.000 74.600 74.600 0.000 0.0 22 F3 18989172.000 76.774 76.774 0.000 0.0 23 G3 23711758.000 95.991 95.991 0.000 0.0 24 H3 21979884.000 88.943 88.943 0.000 0.0 25 A4 1293525.000 4.768 4.768 0.000 0.0 26 B4 1783418.000 6.762 6.762 0.000 0.0 27 C4 1520830.000 5.693 5.693 0.000 0.0 28 D4 80701.000 -0.167 -0.167 0.000 0.0 29 E4 5770485.000 22.985 22.985 0.000 0.0 30 F4 5974880.000 23.817 23.817 0.000 0.0 31 G4 13024253.000 52.502 52.502 0.000 0.0 32 H4 16512069.000 66.694 66.694 0.000 0.0 33 A5 12374803.000 49.859 49.859 0.000 0.0 34 B5 10012874.000 40.248 40.248 0.000 0.0 35 C5 10648321.000 42.834 42.834 0.000 0.0 36 D5 11855346.000 47.745 47.745 0.000 0.0 37 E5 10118879.000 40.680 40.680 0.000 0.0 38 F5 13022324.000 52.494 52.494 0.000 0.0 39 G5 13029220.000 52.522 52.522 0.000 0.0 40 H5 33138526.000 R 134.349 134.349 0.000 0.0 41 A6 8290959.000 33.242 33.242 0.000 0.0 42 B6 8872433.000 35.608 35.608 0.000 0.0 43 C6 12879358.000 51.912 51.912 0.000 0.0 44 D6 9060114.000 36.371 36.371 0.000 0.0 45 E6 13871014.000 55.947 55.947 0.000 0.0 46 F6 12685717.000 51.124 51.124 0.000 0.0 47 G6 13743491.000 55.429 55.429 0.000 0.0 48 H6 26912850.000 R 109.016 109.016 0.000 0.0 49 A7 11567607.000 46.575 46.575 0.000 0.0 50 B7 13454025.000 54.251 54.251 0.000 0.0 51 C7 11008914.000 44.301 44.301 0.000 0.0 52 D7 14134051.000 57.018 57.018 0.000 0.0 53 E7 11459573.000 46.135 46.135 0.000 0.0 54 F7 11174638.000 44.976 44.976 0.000 0.0 55 G7 11724260.000 47.212 47.212 0.000 0.0 56 H7 12227837.000 49.261 49.261 0.000 0.0 57 A8 7632489.000 30.562 30.562 0.000 0.0 58 B8 6813407.000 27.229 27.229 0.000 0.0 59 C8 5617624.000 22.363 22.363 0.000 0.0 60 D8 6019796.000 24.000 24.000 0.000 0.0 61 E8 13026672.000 52.512 52.512 0.000 0.0 62 F8 9153625.000 36.752 36.752 0.000 0.0 63 G8 8056550.000 32.288 32.288 0.000 0.0 64 H8 8735915.000 35.052 35.052 0.000 0.0 65 A9 9454011.000 37.974 37.974 0.000 0.0 66 B9 9526931.000 38.271 38.271 0.000 0.0 67 C9 7053816.000 28.207 28.207 0.000 0.0 68 D9 8211372.000 32.918 32.918 0.000 0.0 69 E9 652498.000 2.160 2.160 0.000 0.0 70 F9 666164.000 2.215 2.215 0.000 0.0 71 G9 795912.000 2.743 2.743 0.000 0.0 72 H9 718925.000 2.430 2.430 0.000 0.0 73 A10 13344289.000 53.804 53.804 0.000 0.0 74 B10 10254874.000 41.233 41.233 0.000 0.0 75 C10 10244067.000 41.189 41.189 0.000 0.0 76 D10 8760478.000 35.152 35.152 0.000 0.0 77 E10 2338360.000 9.020 9.020 0.000 0.0 78 F10 1183662.000 4.321 4.321 0.000 0.0 79 G10 4708589.000 18.664 18.664 0.000 0.0 80 H10 1605690.000 6.038 6.038 0.000 0.0 81 A11 12737726.000 51.336 51.336 0.000 0.0 82 B11 10196860.000 40.997 40.997 0.000 0.0 83 C11 11986472.000 48.279 48.279 0.000 0.0 84 D11 11083045.000 44.603 44.603 0.000 0.0 85 E11 8380970.000 33.608 33.608 0.000 0.0 86 F11 13427965.000 54.145 54.145 0.000 0.0 87 G11 10979906.000 44.183 44.183 0.000 0.0 88 H11 11272242.000 45.373 45.373 0.000 0.0 89 A12 19137366.000 77.377 77.377 0.000 0.0 90 B12 14608479.000 58.948 58.948 0.000 0.0 91 C12 10466649.000 42.095 42.095 0.000 0.0 92 D12 10359153.000 41.657 41.657 0.000 0.0 93 E12 9060852.000 36.374 36.374 0.000 0.0 94 F12 10226765.000 41.119 41.119 0.000 0.0 95 G12 18622258.000 75.281 75.281 0.000 0.0 96 H12 7321901.000 29.298 29.298 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20170816_Quantiflor_attB_PCR_Purified; Date Last Saved: 8/16/2017 1:39:45 PM