##BLOCKS= 3 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 23 25243260 25346216 26039912 1387325 77524 1749213 1243455 270377 180053 494913 119221 1256 10900715 11529094 11722419 886759 1523412 1786527 73687 130401 199554 200442 205728 1246 5673861 5815479 6083963 377784 2038478 548777 1624677 129406 297730 78960 185338 1199 2608241 2844392 2936896 1344492 1354982 312406 28526 203361 161671 137996 157950 1165 1366368 1471625 1379098 1642958 410137 2094458 4067956 445382 98690 148999 186313 1233 652995 672969 728215 1186973 119109 563857 2381348 124484 397970 222211 175804 1161 332591 354639 369304 1934870 160454 1072272 552533 215861 183300 242597 132938 1388 18380 27469 25156 884203 3058452 519504 516886 217377 156061 159422 198914 1202 1 2 3 4 5 6 7 8 9 10 11 12 25243260 25346216 26039912 1387325 77524 1749213 1243455 270377 180053 494913 119221 1256 10900715 11529094 11722419 886759 1523412 1786527 73687 130401 199554 200442 205728 1246 5673861 5815479 6083963 377784 2038478 548777 1624677 129406 297730 78960 185338 1199 2608241 2844392 2936896 1344492 1354982 312406 28526 203361 161671 137996 157950 1165 1366368 1471625 1379098 1642958 410137 2094458 4067956 445382 98690 148999 186313 1233 652995 672969 728215 1186973 119109 563857 2381348 124484 397970 222211 175804 1161 332591 354639 369304 1934870 160454 1072272 552533 215861 183300 242597 132938 1388 18380 27469 25156 884203 3058452 519504 516886 217377 156061 159422 198914 1202 ~End Group: Standards Sample Concentration ng BackCalcConc Wells Value MeanValue SD CV 01 100.000 100.957 A1 25243260.000 25543129.333 433295.220 1.7 101.365 A2 25346216.000 104.111 A3 26039912.000 02 50.000 44.181 B1 10900715.000 11384076.000 429618.472 3.8 46.668 B2 11529094.000 47.434 B3 11722419.000 03 25.000 23.490 C1 5673861.000 5857767.667 208295.850 3.6 24.051 C2 5815479.000 25.113 C3 6083963.000 04 12.500 11.355 D1 2608241.000 2796509.667 169478.806 6.1 12.289 D2 2844392.000 12.656 D3 2936896.000 05 6.250 6.438 E1 1366368.000 1405697.000 57449.013 4.1 6.855 E2 1471625.000 6.489 E3 1379098.000 06 3.125 3.614 F1 652995.000 684726.333 38963.935 5.7 3.694 F2 672969.000 3.912 F3 728215.000 07 1.563 2.346 G1 332591.000 352178.000 18479.813 5.2 2.433 G2 354639.000 2.491 G3 369304.000 08 0.000 1.102 H1 18380.000 23668.333 4723.594 20.0 1.138 H2 27469.000 1.129 H3 25156.000 Group Summaries MinStd Smallest standard value: 23668.333 Min(MeanValue) MaxStd Largest standard value: 25543129.333 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A4 1387325.000 6.521 6.521 0.000 0.0 02 B4 886759.000 4.540 4.540 0.000 0.0 03 C4 377784.000 2.525 2.525 0.000 0.0 04 D4 1344492.000 6.352 6.352 0.000 0.0 05 E4 1642958.000 7.533 7.533 0.000 0.0 06 F4 1186973.000 5.728 5.728 0.000 0.0 07 G4 1934870.000 8.689 8.689 0.000 0.0 08 H4 884203.000 4.530 4.530 0.000 0.0 09 A5 77524.000 1.336 1.336 0.000 0.0 10 B5 1523412.000 7.060 7.060 0.000 0.0 11 C5 2038478.000 9.099 9.099 0.000 0.0 12 D5 1354982.000 6.393 6.393 0.000 0.0 13 E5 410137.000 2.653 2.653 0.000 0.0 14 F5 119109.000 1.501 1.501 0.000 0.0 15 G5 160454.000 1.665 1.665 0.000 0.0 16 H5 3058452.000 13.137 13.137 0.000 0.0 17 A6 1749213.000 7.954 7.954 0.000 0.0 18 B6 1786527.000 8.102 8.102 0.000 0.0 19 C6 548777.000 3.202 3.202 0.000 0.0 20 D6 312406.000 2.266 2.266 0.000 0.0 21 E6 2094458.000 9.321 9.321 0.000 0.0 22 F6 563857.000 3.262 3.262 0.000 0.0 23 G6 1072272.000 5.274 5.274 0.000 0.0 24 H6 519504.000 3.086 3.086 0.000 0.0 25 A7 1243455.000 5.952 5.952 0.000 0.0 26 B7 73687.000 1.321 1.321 0.000 0.0 27 C7 1624677.000 7.461 7.461 0.000 0.0 28 D7 28526.000 1.142 1.142 0.000 0.0 29 E7 4067956.000 17.133 17.133 0.000 0.0 30 F7 2381348.000 10.456 10.456 0.000 0.0 31 G7 552533.000 3.217 3.217 0.000 0.0 32 H7 516886.000 3.076 3.076 0.000 0.0 33 A8 270377.000 2.100 2.100 0.000 0.0 34 B8 130401.000 1.546 1.546 0.000 0.0 35 C8 129406.000 1.542 1.542 0.000 0.0 36 D8 203361.000 1.835 1.835 0.000 0.0 37 E8 445382.000 2.793 2.793 0.000 0.0 38 F8 124484.000 1.522 1.522 0.000 0.0 39 G8 215861.000 1.884 1.884 0.000 0.0 40 H8 217377.000 1.890 1.890 0.000 0.0 41 A9 180053.000 1.742 1.742 0.000 0.0 42 B9 199554.000 1.820 1.820 0.000 0.0 43 C9 297730.000 2.208 2.208 0.000 0.0 44 D9 161671.000 1.670 1.670 0.000 0.0 45 E9 98690.000 1.420 1.420 0.000 0.0 46 F9 397970.000 2.605 2.605 0.000 0.0 47 G9 183300.000 1.755 1.755 0.000 0.0 48 H9 156061.000 1.647 1.647 0.000 0.0 49 A10 494913.000 2.989 2.989 0.000 0.0 50 B10 200442.000 1.823 1.823 0.000 0.0 51 C10 78960.000 1.342 1.342 0.000 0.0 52 D10 137996.000 1.576 1.576 0.000 0.0 53 E10 148999.000 1.619 1.619 0.000 0.0 54 F10 222211.000 1.909 1.909 0.000 0.0 55 G10 242597.000 1.990 1.990 0.000 0.0 56 H10 159422.000 1.661 1.661 0.000 0.0 57 A11 119221.000 1.502 1.502 0.000 0.0 58 B11 205728.000 1.844 1.844 0.000 0.0 59 C11 185338.000 1.763 1.763 0.000 0.0 60 D11 157950.000 1.655 1.655 0.000 0.0 61 E11 186313.000 1.767 1.767 0.000 0.0 62 F11 175804.000 1.725 1.725 0.000 0.0 63 G11 132938.000 1.556 1.556 0.000 0.0 64 H11 198914.000 1.817 1.817 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20171018_Quantiflor_DNA_for_qPCR; Date Last Saved: 10/18/2017 3:40:56 PM