##BLOCKS= 4 Plate: Plate1 1.3 TimeFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 23 16494951 14678627 16138794 1182 1242 1293 1203 1266 1218 1227 1254 1188 2373846 2905128 2189056 1234 1292 1331 1263 1256 1142 1224 1220 1246 243114 256612 249961 1146 1242 1119 1162 1251 1273 1287 1776 1229 180084 172830 159336 1259 1257 1199 1204 1356 1199 1328 1151 1197 138640 154682 137597 1309 1253 1164 1212 1267 1281 1156 1274 1242 127194 122040 121830 1267 1284 1294 1181 1311 1341 1211 1160 1153 102447 105051 103448 1242 1348 1299 1246 1374 1246 1178 1205 1188 98614 99054 95548 1282 1275 1157 1201 1263 1165 1216 1132 1182 ~End Plate: Plate2 1.3 TimeFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 23 19892008 19521408 19990556 17465062 13570590 11896290 13004424 9428895 9681435 14425864 13469714 8749245 20546438 16874342 18879370 15199620 10256097 14898117 13108404 11692209 5699304 10214223 12116647 3473198 17937814 8334248 20874570 6883259 14409666 13470014 13247324 12178068 9645501 13592988 11352420 12311482 17891358 19871502 18344326 15926843 13426367 13810752 14898523 10946941 13802602 14201681 11761267 10496527 18140234 18691110 17320706 16497709 14205119 15440968 13848996 5407492 8450259 980332 10897446 10750658 115427 18523768 18061494 16431758 15207610 14604497 12751686 5116024 2134750 824138 4976450 11101112 112702 18880456 19355970 15811653 14658765 13666643 12996990 8299950 3024969 2586955 6845722 8890558 120088 17837420 18223734 603991 15485446 12084433 10659526 10396320 2356389 1077377 7064391 102154 ~End Group: Standards Sample Concentration ng BackCalcConc Wells Value MeanValue SD CV 01 100.000 120.727 A1 16494951.000 15770790.667 962459.380 6.1 108.372 A2 14678627.000 118.304 A3 16138794.000 02 50.000 24.670 B1 2373846.000 2489343.333 371745.212 14.9 28.284 B2 2905128.000 23.413 B3 2189056.000 03 25.000 10.176 C1 243114.000 249895.667 6749.237 2.7 10.267 C2 256612.000 10.222 C3 249961.000 04 12.500 9.747 D1 180084.000 170750.000 10529.230 6.2 9.698 D2 172830.000 9.606 D3 159336.000 05 6.250 9.465 E1 138640.000 143639.667 9577.150 6.7 9.574 E2 154682.000 9.458 E3 137597.000 06 3.125 9.387 F1 127194.000 123688.000 3038.100 2.5 9.352 F2 122040.000 9.351 F3 121830.000 07 1.563 9.219 G1 102447.000 103648.667 1313.546 1.3 9.236 G2 105051.000 9.226 G3 103448.000 08 0.000 9.193 H1 98614.000 97738.667 1909.886 2.0 9.196 H2 99054.000 9.172 H3 95548.000 Group Summaries MinStd Smallest standard value: 97738.667 Min(MeanValue) MaxStd Largest standard value: 15770790.667 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 19892008.000 R 143.835 143.835 0.000 0.0 02 B1 20546438.000 R 148.287 148.287 0.000 0.0 03 C1 17937814.000 R 130.542 130.542 0.000 0.0 04 D1 17891358.000 R 130.226 130.226 0.000 0.0 05 E1 18140234.000 R 131.919 131.919 0.000 0.0 06 F1 115427.000 9.307 9.307 0.000 0.0 07 G1 112702.000 9.289 9.289 0.000 0.0 08 H1 120088.000 9.339 9.339 0.000 0.0 09 A2 19521408.000 R 141.314 141.314 0.000 0.0 10 B2 16874342.000 R 123.308 123.308 0.000 0.0 11 C2 8334248.000 65.215 65.215 0.000 0.0 12 D2 19871502.000 R 143.696 143.696 0.000 0.0 13 E2 18691110.000 R 135.666 135.666 0.000 0.0 14 F2 18523768.000 R 134.528 134.528 0.000 0.0 15 G2 18880456.000 R 136.954 136.954 0.000 0.0 16 H2 17837420.000 R 129.859 129.859 0.000 0.0 17 A3 19990556.000 R 144.506 144.506 0.000 0.0 18 B3 18879370.000 R 136.947 136.947 0.000 0.0 19 C3 20874570.000 R 150.519 150.519 0.000 0.0 20 D3 18344326.000 R 133.307 133.307 0.000 0.0 21 E3 17320706.000 R 126.344 126.344 0.000 0.0 22 F3 18061494.000 R 131.383 131.383 0.000 0.0 23 G3 19355970.000 R 140.189 140.189 0.000 0.0 24 H3 18223734.000 R 132.487 132.487 0.000 0.0 25 A4 17465062.000 R 127.326 127.326 0.000 0.0 26 B4 15199620.000 111.916 111.916 0.000 0.0 27 C4 6883259.000 55.345 55.345 0.000 0.0 28 D4 15926843.000 R 116.863 116.863 0.000 0.0 29 E4 16497709.000 R 120.746 120.746 0.000 0.0 30 F4 16431758.000 R 120.297 120.297 0.000 0.0 31 G4 15811653.000 R 116.079 116.079 0.000 0.0 32 H4 603991.000 12.630 12.630 0.000 0.0 33 A5 13570590.000 100.835 100.835 0.000 0.0 34 B5 10256097.000 78.288 78.288 0.000 0.0 35 C5 14409666.000 106.542 106.542 0.000 0.0 36 D5 13426367.000 99.853 99.853 0.000 0.0 37 E5 14205119.000 105.151 105.151 0.000 0.0 38 F5 15207610.000 111.970 111.970 0.000 0.0 39 G5 14658765.000 108.237 108.237 0.000 0.0 40 H5 15485446.000 113.860 113.860 0.000 0.0 41 A6 11896290.000 89.445 89.445 0.000 0.0 42 B6 14898117.000 109.865 109.865 0.000 0.0 43 C6 13470014.000 100.150 100.150 0.000 0.0 44 D6 13810752.000 102.468 102.468 0.000 0.0 45 E6 15440968.000 113.558 113.558 0.000 0.0 46 F6 14604497.000 107.868 107.868 0.000 0.0 47 G6 13666643.000 101.488 101.488 0.000 0.0 48 H6 12084433.000 90.725 90.725 0.000 0.0 49 A7 13004424.000 96.983 96.983 0.000 0.0 50 B7 13108404.000 97.691 97.691 0.000 0.0 51 C7 13247324.000 98.636 98.636 0.000 0.0 52 D7 14898523.000 109.868 109.868 0.000 0.0 53 E7 13848996.000 102.728 102.728 0.000 0.0 54 F7 12751686.000 95.264 95.264 0.000 0.0 55 G7 12996990.000 96.933 96.933 0.000 0.0 56 H7 10659526.000 81.032 81.032 0.000 0.0 57 A8 9428895.000 72.661 72.661 0.000 0.0 58 B8 11692209.000 88.057 88.057 0.000 0.0 59 C8 12178068.000 91.362 91.362 0.000 0.0 60 D8 10946941.000 82.987 82.987 0.000 0.0 61 E8 5407492.000 45.306 45.306 0.000 0.0 62 F8 5116024.000 43.323 43.323 0.000 0.0 63 G8 8299950.000 64.981 64.981 0.000 0.0 64 H8 10396320.000 79.242 79.242 0.000 0.0 65 A9 9681435.000 74.379 74.379 0.000 0.0 66 B9 5699304.000 47.291 47.291 0.000 0.0 67 C9 9645501.000 74.134 74.134 0.000 0.0 68 D9 13802602.000 102.413 102.413 0.000 0.0 69 E9 8450259.000 66.004 66.004 0.000 0.0 70 F9 2134750.000 23.043 23.043 0.000 0.0 71 G9 3024969.000 29.099 29.099 0.000 0.0 72 H9 2356389.000 24.551 24.551 0.000 0.0 73 A10 14425864.000 106.652 106.652 0.000 0.0 74 B10 10214223.000 78.003 78.003 0.000 0.0 75 C10 13592988.000 100.987 100.987 0.000 0.0 76 D10 14201681.000 105.127 105.127 0.000 0.0 77 E10 980332.000 15.190 15.190 0.000 0.0 78 F10 824138.000 14.128 14.128 0.000 0.0 79 G10 2586955.000 26.119 26.119 0.000 0.0 80 H10 1077377.000 15.851 15.851 0.000 0.0 81 A11 13469714.000 100.148 100.148 0.000 0.0 82 B11 12116647.000 90.944 90.944 0.000 0.0 83 C11 11352420.000 85.746 85.746 0.000 0.0 84 D11 11761267.000 88.527 88.527 0.000 0.0 85 E11 10897446.000 82.651 82.651 0.000 0.0 86 F11 4976450.000 42.374 42.374 0.000 0.0 87 G11 6845722.000 55.089 55.089 0.000 0.0 88 H11 7064391.000 56.577 56.577 0.000 0.0 89 A12 8749245.000 68.038 68.038 0.000 0.0 90 B12 3473198.000 32.148 32.148 0.000 0.0 91 C12 12311482.000 92.270 92.270 0.000 0.0 92 D12 10496527.000 79.924 79.924 0.000 0.0 93 E12 10750658.000 81.652 81.652 0.000 0.0 94 F12 11101112.000 84.036 84.036 0.000 0.0 95 G12 8890558.000 68.999 68.999 0.000 0.0 96 H12 102154.000 9.217 9.217 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20180920-Quantifluor_pENTR_minipreps; Date Last Saved: 9/20/2018 2:07:08 PM