Load time: 04/24/2011 11:20:17 AM Read time: 04/24/2011 11:20:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1499 Comment: standards bg Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28126052 cps Min counts: 70728 counts Well list: r0(a1:h11,a12:g12,h12) = cps(f2(a1:h11,a12:g12,h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8222052.00 6479401.00 4715708.00 5534643.00 5310829.00 9953441.00 9031381.00 9296232.00 10985229.00 10901916.00 11080839.00 3400440.00 B 15392700.00 6517645.00 4617861.00 6110510.00 4290393.00 7290110.00 9719093.00 9584268.00 10583265.00 6466146.00 7178661.00 161979.00 C 18141150.00 6944060.00 5091012.00 4651005.00 3930968.00 7411570.00 7476268.00 8929005.00 8738917.00 7336197.00 9721105.00 707966.00 D 11590695.00 7175537.00 4221547.00 4525300.00 4921799.00 6373451.00 7465675.00 7683837.00 8713072.00 9801158.00 8983294.00 916171.00 E 17234104.00 6558129.00 4165110.00 3742568.00 3993396.00 7353048.00 5335039.00 7597737.00 8691125.00 8524773.00 9185052.00 507147.00 F 25342196.00 5821056.00 5177633.00 4104057.00 4268785.00 6853012.00 5567865.00 6711598.00 8814324.00 9033808.00 9910546.00 27110032.00 G 22146664.00 6017967.00 5176100.00 2433714.00 4868351.00 4589971.00 5786909.00 6729467.00 8155693.00 2583467.00 7476776.00 28126052.00 H 26477478.00 7411043.00 6134000.00 5021364.00 8431104.00 9481684.00 5082809.00 8892065.00 8005014.00 7495331.00 6836838.00 141456.00 Well list: r1(a1:h11,a12:g12) = cps(f2(a1:h11,a12:g12) - avg(f2(h12))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8080596.00 6337945.00 4574252.00 5393187.00 5169373.00 9811985.00 8889925.00 9154776.00 10843773.00 10760460.00 10939383.00 3258984.00 B 15251244.00 6376189.00 4476405.00 5969054.00 4148937.00 7148654.00 9577637.00 9442812.00 10441809.00 6324690.00 7037205.00 20523.00 C 17999694.00 6802604.00 4949556.00 4509549.00 3789512.00 7270114.00 7334812.00 8787549.00 8597461.00 7194741.00 9579649.00 566510.00 D 11449239.00 7034081.00 4080091.00 4383844.00 4780343.00 6231995.00 7324219.00 7542381.00 8571616.00 9659702.00 8841838.00 774715.00 E 17092648.00 6416673.00 4023654.00 3601112.00 3851940.00 7211592.00 5193583.00 7456281.00 8549669.00 8383317.00 9043596.00 365691.00 F 25200740.00 5679600.00 5036177.00 3962601.00 4127329.00 6711556.00 5426409.00 6570142.00 8672868.00 8892352.00 9769090.00 26968576.00 G 22005208.00 5876511.00 5034644.00 2292258.00 4726895.00 4448515.00 5645453.00 6588011.00 8014237.00 2442011.00 7335320.00 27984596.00 H 26336022.00 7269587.00 5992544.00 4879908.00 8289648.00 9340228.00 4941353.00 8750609.00 7863558.00 7353875.00 6695382.00