Load time: 04/24/2011 11:22:37 AM Read time: 04/24/2011 11:22:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1500 Comment: standards bg Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29020992 cps Min counts: 70661 counts Well list: r0(a1:h11,a12:g12,h12) = cps(f2(a1:h11,a12:g12,h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8282158.00 6484126.00 4730055.00 5557777.00 5330648.00 10008912.00 9020756.00 9238698.00 10976509.00 10842587.00 11046823.00 3416041.00 B 15667193.00 6476027.00 4627092.00 6108381.00 4321260.00 7263005.00 9641563.00 9606512.00 10610958.00 6451526.00 7194902.00 161408.00 C 18511940.00 6935692.00 5075409.00 4695757.00 3915402.00 7397509.00 7553880.00 8897230.00 8815648.00 7252292.00 9529701.00 707471.00 D 11660140.00 7182040.00 4231840.00 4541859.00 4933694.00 6390740.00 7451235.00 7642668.00 8747043.00 9844625.00 8973876.00 915695.00 E 17461280.00 6608940.00 4208522.00 3769577.00 4000950.00 7366135.00 5361363.00 7617888.00 8686121.00 8439207.00 9204946.00 504368.00 F 25975556.00 5819688.00 5202219.00 4136880.00 4285685.00 6841397.00 5596258.00 6641400.00 8837639.00 8878439.00 10202118.00 28028900.00 G 22557630.00 6019212.00 5141929.00 2445023.00 4876622.00 4602919.00 5803063.00 6779717.00 8182848.00 2572662.00 7564719.00 29020992.00 H 27109274.00 7456622.00 6152390.00 5052820.00 8531591.00 9453361.00 5056308.00 8937406.00 8007078.00 7445921.00 6863913.00 141322.00 Well list: r1(a1:h11,a12:g12) = cps(f2(a1:h11,a12:g12) - avg(f2(h12))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8140836.00 6342804.00 4588733.00 5416455.00 5189326.00 9867590.00 8879434.00 9097376.00 10835187.00 10701265.00 10905501.00 3274719.00 B 15525871.00 6334705.00 4485770.00 5967059.00 4179938.00 7121683.00 9500241.00 9465190.00 10469636.00 6310204.00 7053580.00 20086.00 C 18370618.00 6794370.00 4934087.00 4554435.00 3774080.00 7256187.00 7412558.00 8755908.00 8674326.00 7110970.00 9388379.00 566149.00 D 11518818.00 7040718.00 4090518.00 4400537.00 4792372.00 6249418.00 7309913.00 7501346.00 8605721.00 9703303.00 8832554.00 774373.00 E 17319958.00 6467618.00 4067200.00 3628255.00 3859628.00 7224813.00 5220041.00 7476566.00 8544799.00 8297885.00 9063624.00 363046.00 F 25834234.00 5678366.00 5060897.00 3995558.00 4144363.00 6700075.00 5454936.00 6500078.00 8696317.00 8737117.00 10060796.00 27887578.00 G 22416308.00 5877890.00 5000607.00 2303701.00 4735300.00 4461597.00 5661741.00 6638395.00 8041526.00 2431340.00 7423397.00 28879670.00 H 26967952.00 7315300.00 6011068.00 4911498.00 8390269.00 9312039.00 4914986.00 8796084.00 7865756.00 7304599.00 6722591.00