Load time: 04/24/2011 11:17:05 AM Read time: 04/24/2011 11:17:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1498 Comment: standards bg Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 147530608 cps Min counts: 71889 counts Well list: r0(a1:h3,a4:h4) = cps(f2(a1:h3,a4:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 147530608.00 135366144.00 102157240.00 143778.00 B 58948452.00 59955968.00 73112440.00 144559.00 C 45668516.00 37592968.00 40007860.00 145987.00 D 27885672.00 29224590.00 27292010.00 146520.00 E 15138879.00 15451257.00 14745808.00 143835.00 F 10021568.00 9630667.00 8723374.00 144654.00 G 5377182.00 5111699.00 5006678.00 144578.00 H 3206942.00 3684725.00 3350922.00 144902.00 Well list: r1(a1:h3) = cps(f2(a1:h3) - avg(f2(a4:h4))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 147385756.38 135221292.38 102012388.38 B 58803600.38 59811116.38 72967588.38 C 45523664.38 37448116.38 39863008.38 D 27740820.38 29079738.38 27147158.38 E 14994027.38 15306405.38 14600956.38 F 9876716.38 9485815.38 8578522.38 G 5232330.38 4966847.38 4861826.38 H 3062090.38 3539873.38 3206070.38