Load time: 04/26/2012 09:31:28 PM Read time: 04/26/2012 09:31:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2518 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 94830392 cps Min counts: 30719 counts Well list: r0(a1:h3,a4:d4) = cps(f2(a1:h3,a4:d4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 82209584.00 94830392.00 79350200.00 68560.00 B 50122576.00 47224452.00 48329004.00 174026.00 C 22674012.00 32313880.00 32402120.00 61439.00 D 16085550.00 21839578.00 14245251.00 125674.00 E 7122323.00 8383155.00 8643030.00 F 4842602.00 4494323.00 3086574.00 G 2573921.00 2780573.00 2300597.00 H 1803784.00 1848428.00 1470080.00 Well list: r1(a1:h3) = cps(f2(a1:h3) - avg(f2(a4:d4))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 82102159.25 94722967.25 79242775.25 B 50015151.25 47117027.25 48221579.25 C 22566587.25 32206455.25 32294695.25 D 15978125.25 21732153.25 14137826.25 E 7014898.25 8275730.25 8535605.25 F 4735177.25 4386898.25 2979149.25 G 2466496.25 2673148.25 2193172.25 H 1696359.25 1741003.25 1362655.25 Load time: 04/26/2012 09:34:02 PM Read time: 04/26/2012 09:34:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2519 Comment: LS RILs Plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 269703264 cps Min counts: 773616 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 59870980.00 29668684.00 231712000.00 257803536.00 234415136.00 218104576.00 172512912.00 85184144.00 36928052.00 215354176.00 82593128.00 120581840.00 B 71024848.00 217173904.00 235657632.00 248631488.00 162942176.00 76096208.00 108072704.00 74851176.00 191821680.00 125581024.00 105928480.00 122835328.00 C 122183472.00 232554832.00 237992032.00 48785860.00 225636368.00 93692240.00 33502816.00 62367840.00 218801616.00 157665408.00 118058976.00 99469856.00 D 199319008.00 221001824.00 231601008.00 198012976.00 204931632.00 113598456.00 163196544.00 61012720.00 215228384.00 144086864.00 213582416.00 126652176.00 E 32634764.00 242840464.00 249739104.00 251394672.00 212625584.00 66451076.00 36477596.00 114759520.00 207972704.00 107253136.00 212549648.00 172106096.00 F 178833744.00 216287424.00 178825472.00 160491440.00 224379856.00 35517968.00 25115228.00 131188216.00 121266056.00 200704864.00 189714288.00 233010112.00 G 32062768.00 214399264.00 269703264.00 43061004.00 230222544.00 61890832.00 72026664.00 96369640.00 212748352.00 45899996.00 163690320.00 197124944.00 H 54556128.00 74563320.00 143784256.00 27845070.00 223137248.00 67632136.00 220290688.00 143944784.00 201725376.00 105403144.00 209771648.00 208634544.00 Load time: 04/26/2012 09:36:11 PM Read time: 04/26/2012 09:36:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2520 Comment: LS RILs Plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 293281088 cps Min counts: 325123 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 263664080.00 242323216.00 269374592.00 284606272.00 263341008.00 246578992.00 273208384.00 112297408.00 208673920.00 238557120.00 214227088.00 650246.00 B 251084624.00 234074704.00 272890336.00 250710336.00 255251696.00 259986832.00 271539776.00 256734112.00 249843968.00 253271200.00 111962608.00 12680581.00 C 244097072.00 236074720.00 284250784.00 271778272.00 266562208.00 279799232.00 249151968.00 202422112.00 262287472.00 275239936.00 260065488.00 256921680.00 D 277202560.00 229600784.00 249368816.00 162153232.00 261445376.00 282486272.00 275063776.00 155179616.00 258605024.00 250604752.00 238087248.00 15562883.00 E 267715264.00 235499808.00 262831024.00 266920912.00 274429536.00 202522816.00 269844384.00 248352112.00 248943504.00 204282256.00 224191280.00 2572644.00 F 274371136.00 227060208.00 277640256.00 283438464.00 244143296.00 284637696.00 282455296.00 266158688.00 259611680.00 232610736.00 252964768.00 259220224.00 G 293281088.00 247517776.00 265701616.00 255927248.00 268700928.00 272103296.00 289418976.00 252403552.00 266387808.00 184475616.00 248647472.00 211359184.00 H 273150496.00 239117504.00 238564480.00 289798400.00 260109216.00 275396704.00 225735120.00 244181824.00 240112016.00 243873920.00 249892400.00 258769344.00 Load time: 04/26/2012 09:38:37 PM Read time: 04/26/2012 09:38:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2521 Comment: LS RILs Plate2 dil 6 + 60 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 270950176 cps Min counts: 57766 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 140005936.00 240927072.00 71570224.00 270950176.00 263314112.00 68638424.00 262804240.00 11554814.00 21023626.00 120736144.00 30367212.00 115532.00 B 204969632.00 53921100.00 258977264.00 243214560.00 143259728.00 66678464.00 242916720.00 65699392.00 232673728.00 25133834.00 12255782.00 1170152.00 C 64812112.00 233728160.00 153112640.00 268207200.00 66171436.00 244560064.00 256802272.00 37316188.00 205410448.00 189277952.00 107425896.00 218820176.00 D 235299248.00 227923168.00 132181176.00 15851992.00 256368272.00 253844640.00 248360080.00 12570121.00 64242688.00 70765616.00 44098572.00 1133152.00 E 224751424.00 205973776.00 249545760.00 108337568.00 262795328.00 19081956.00 99925968.00 59366964.00 168543984.00 19751046.00 22238680.00 289709.00 F 242878560.00 96091920.00 255310672.00 258035712.00 118067984.00 269628096.00 247970016.00 93643480.00 238888624.00 41549412.00 53824692.00 32806368.00 G 264430144.00 244575536.00 172178864.00 218913040.00 260021792.00 245717456.00 199792592.00 220888224.00 238925504.00 17303690.00 66669268.00 22484350.00 H 228664224.00 222035728.00 66888316.00 259663968.00 247763552.00 172964160.00 70890128.00 45538220.00 83039064.00 39402300.00 57150856.00 32615088.00 Load time: 04/26/2012 09:40:39 PM Read time: 04/26/2012 09:40:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2522 Comment: PA PCR Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42169300 cps Min counts: 39681 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20811594.00 14256880.00 22624940.00 22328536.00 24538232.00 21654654.00 29847596.00 19828754.00 23157536.00 22152264.00 32502996.00 19302396.00 B 24408862.00 14032950.00 24737056.00 31186054.00 24298996.00 25600260.00 23764112.00 19846200.00 23513978.00 21198816.00 23792322.00 83991.00 C 27714594.00 21014546.00 21311172.00 40322156.00 25829330.00 25070870.00 34739632.00 23255620.00 20242108.00 19394824.00 24569408.00 80345.00 D 23348622.00 16642647.00 32475708.00 25822606.00 27438378.00 21614724.00 24316868.00 21123970.00 22492458.00 21028940.00 24282218.00 82310.00 E 24005088.00 19727324.00 27009784.00 24478680.00 25296808.00 25213782.00 11977403.00 24999980.00 24596436.00 24298310.00 29295792.00 81082.00 F 37424504.00 22040116.00 29264206.00 17120000.00 23667996.00 24081608.00 28766694.00 20919702.00 24987464.00 17875232.00 15731498.00 80610.00 G 31799444.00 24390930.00 28368892.00 25537724.00 37954024.00 22236570.00 20251168.00 24063782.00 29322106.00 22718892.00 28842084.00 80572.00 H 21875150.00 29694650.00 25997374.00 26311712.00 36629068.00 30445730.00 99459.00 42169300.00 37833744.00 36515472.00 29247942.00 79363.00 Load time: 04/26/2012 09:42:06 PM Read time: 04/26/2012 09:42:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2523 Comment: PA PCR Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42379520 cps Min counts: 39625 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20832890.00 14279960.00 22568838.00 22373484.00 24752334.00 21613274.00 29773732.00 19835872.00 23132608.00 21977126.00 32226788.00 19214852.00 B 24480694.00 13982627.00 24500154.00 30912894.00 24469932.00 25592806.00 23885688.00 19896734.00 23377690.00 21361896.00 23657286.00 82347.00 C 27458830.00 20824870.00 21588304.00 40932540.00 25978432.00 24985768.00 34690400.00 23163082.00 20319678.00 19430530.00 24573856.00 80043.00 D 23727450.00 16637171.00 32264418.00 25785596.00 27322382.00 21609408.00 24489450.00 21075688.00 22686512.00 21061402.00 24409860.00 81611.00 E 24048448.00 19689966.00 26635664.00 24677718.00 25220030.00 24889946.00 11970139.00 24947256.00 24690240.00 24312132.00 29261772.00 83613.00 F 37357096.00 21974258.00 29304778.00 17227510.00 23809092.00 23833514.00 28554862.00 20824870.00 24580336.00 17968816.00 15788422.00 79590.00 G 32065294.00 24540880.00 28309202.00 25424924.00 37463284.00 22250766.00 20198872.00 24171526.00 29573416.00 22507838.00 28808146.00 82499.00 H 21839148.00 30019590.00 25774686.00 26653742.00 36656660.00 30617558.00 98874.00 42379520.00 37257244.00 36671776.00 29403466.00 79250.00 Load time: 04/26/2012 09:43:57 PM Read time: 04/26/2012 09:43:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2524 Comment: PA PCR Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 748648 cps Min counts: 99242 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 323026.00 198484.00 446850.00 407149.00 397404.00 229894.00 416499.00 413344.00 469704.00 748648.00 378271.00 314942.00 B 457597.00 457805.00 403901.00 293619.00 497581.00 459316.00 438464.00 443432.00 475653.00 315018.00 448323.00 360309.00 C 310542.00 286064.00 380745.00 449740.00 391549.00 407640.00 419860.00 436273.00 448153.00 354908.00 376684.00 413439.00 D 430343.00 443790.00 343519.00 482755.00 397763.00 385354.00 434706.00 483416.00 434215.00 397215.00 377818.00 374701.00 E 422014.00 447776.00 408415.00 442487.00 308521.00 366674.00 442336.00 339326.00 435423.00 368846.00 426169.00 391416.00 F 397933.00 436122.00 236391.00 448059.00 378781.00 469099.00 455652.00 499319.00 369885.00 376609.00 372076.00 426698.00 G 429795.00 508913.00 483945.00 376401.00 362576.00 318191.00 463131.00 411947.00 413250.00 291881.00 338948.00 427264.00 H 409680.00 456993.00 459486.00 361197.00 421863.00 354927.00 414497.00 382842.00 461658.00 397234.00 418633.00 453990.00 Load time: 04/26/2012 09:46:20 PM Read time: 04/26/2012 09:46:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2525 Comment: Adapters GBS Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 746079 cps Min counts: 98571 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 315641.00 197143.00 445830.00 385731.00 343821.00 209175.00 394608.00 385486.00 445264.00 746079.00 370320.00 314376.00 B 418973.00 440976.00 364465.00 291277.00 436576.00 408963.00 405865.00 392720.00 412249.00 272031.00 393097.00 357835.00 C 302024.00 271672.00 346427.00 398291.00 336039.00 350526.00 357363.00 367713.00 372605.00 296339.00 320325.00 371113.00 D 417235.00 431231.00 309276.00 413628.00 345634.00 329599.00 374135.00 424337.00 369375.00 330505.00 323498.00 341781.00 E 408283.00 440126.00 358761.00 413987.00 265704.00 312582.00 370187.00 300626.00 368601.00 312903.00 365333.00 360064.00 F 383427.00 423940.00 209968.00 373681.00 317964.00 388678.00 397196.00 431287.00 317757.00 316340.00 319022.00 397441.00 G 412457.00 503682.00 451270.00 357816.00 320231.00 290937.00 441581.00 376930.00 371302.00 272106.00 304951.00 440636.00 H 403995.00 444659.00 450344.00 346918.00 375230.00 344992.00 405034.00 375759.00 447662.00 397460.00 411512.00 449041.00 Load time: 04/26/2012 09:51:30 PM Read time: 04/26/2012 09:51:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2526 Comment: Plate 1 Min Sensitivity Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 0 A/D converter gain: x1 Integrating gain: x1 Max cps: 271981408 cps Min counts: 7770047 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 64389484.00 29538926.00 236708368.00 259731408.00 237441824.00 226087040.00 185337360.00 95724768.00 41904572.00 222687216.00 89675976.00 125379960.00 B 73548712.00 217542320.00 238796224.00 250802816.00 180065168.00 87642344.00 123070344.00 83930736.00 197556784.00 139178816.00 114206952.00 132823808.00 C 125655920.00 237970512.00 243295232.00 48870428.00 245878992.00 99230768.00 34511424.00 69483800.00 223772960.00 174625840.00 126856040.00 109457168.00 D 214321872.00 237931904.00 259793904.00 201587968.00 227179072.00 131102408.00 185109536.00 69672520.00 222215296.00 163363264.00 220023632.00 136918080.00 E 33263310.00 249011216.00 258814336.00 257613872.00 227284064.00 73191848.00 40289684.00 121570544.00 215773488.00 117199688.00 226031808.00 175426960.00 F 185938752.00 224517216.00 174154192.00 180610960.00 246530384.00 37346192.00 27293938.00 145799440.00 129989320.00 210356592.00 197082720.00 232713456.00 G 32944948.00 225677296.00 271981408.00 45287224.00 239496944.00 67826136.00 81282248.00 106765880.00 214125792.00 45676524.00 168587520.00 200884368.00 H 55594404.00 72460952.00 141216576.00 28814138.00 228254192.00 75912872.00 232375920.00 152299792.00 202055552.00 113890560.00 216780624.00 216412912.00 Load time: 04/27/2012 02:21:30 PM Read time: 04/27/2012 02:21:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2528 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 103848120 cps Min counts: 42130 counts Well list: r0(a1:h2,a3,b3,c3) = cps(f2(a1:h2,a3,b3,c3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103848120.00 76076400.00 84261.00 B 55793236.00 45212612.00 88942.00 C 28924402.00 23885040.00 91547.00 D 10920433.00 11948520.00 E 10268813.00 8465316.00 F 5558310.00 5059248.00 G 3161456.00 3146715.00 H 1873053.00 2163774.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3,b3,c3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103759870.00 75988150.00 B 55704986.00 45124362.00 C 28836152.00 23796790.00 D 10832183.00 11860270.00 E 10180563.00 8377066.00 F 5470060.00 4970998.00 G 3073206.00 3058465.00 H 1784803.00 2075524.00 Load time: 04/27/2012 02:22:59 PM Read time: 04/27/2012 02:22:59 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2529 Comment: standards 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 105919560 cps Min counts: 41668 counts Well list: r0(a1:h2,a3,b3,c3) = cps(f2(a1:h2,a3,b3,c3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 105919560.00 76265504.00 83336.00 B 56539900.00 44661796.00 86167.00 C 29601252.00 23489124.00 91358.00 D 10743525.00 11878814.00 E 10300032.00 8414085.00 F 5495312.00 5008734.00 G 3172383.00 3146543.00 H 1875902.00 2164411.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3,b3,c3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 105832606.33 76178550.33 B 56452946.33 44574842.33 C 29514298.33 23402170.33 D 10656571.33 11791860.33 E 10213078.33 8327131.33 F 5408358.33 4921780.33 G 3085429.33 3059589.33 H 1788948.33 2077457.33 Load time: 04/27/2012 02:24:38 PM Read time: 04/27/2012 02:24:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2530 Comment: GBS plate 1 dil 5 + 95 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35335728 cps Min counts: 615641 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2662916.00 1231282.00 10407248.00 11226847.00 9546549.00 12773584.00 8940529.00 4320962.00 1867316.00 10950627.00 3843916.00 4736180.00 B 3416194.00 7198106.00 7332196.00 6026537.00 7860306.00 4295238.00 3976841.00 4234133.00 6133130.00 5455451.00 4145747.00 4831038.00 C 4561568.00 18401522.00 10940709.00 2306229.00 13448156.00 4321135.00 1866507.00 2669213.00 12585959.00 6279456.00 6065723.00 5198225.00 D 8541736.00 15041228.00 12310804.00 6064039.00 17086624.00 4473096.00 6711842.00 2681687.00 11534067.00 4941409.00 12120929.00 6495524.00 E 1907275.00 14852631.00 8088089.00 15943754.00 15495402.00 3637392.00 2005705.00 4979472.00 7538190.00 3820534.00 11366314.00 9628152.00 F 8165152.00 7134854.00 5009364.00 3838115.00 11151099.00 2466638.00 1552395.00 5920219.00 4770167.00 10157500.00 4761290.00 12044324.00 G 1837817.00 6983106.00 14176210.00 2407020.00 35335728.00 3356562.00 3032953.00 4412967.00 5149001.00 2076676.00 6011257.00 5428526.00 H 3274799.00 3995791.00 5763671.00 1349425.00 11472555.00 3710847.00 4339978.00 7284776.00 4914662.00 5117330.00 7418014.00 16336012.00 Load time: 04/27/2012 02:26:20 PM Read time: 04/27/2012 02:26:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2531 Comment: GBS plate 1 dil 5 + 95 2nd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35596844 cps Min counts: 613753 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2656233.00 1227506.00 10485811.00 11124705.00 9737518.00 12880797.00 8987369.00 4311261.00 1860914.00 11154704.00 3866782.00 4744679.00 B 3417575.00 7152273.00 7191109.00 6011528.00 8015181.00 4288842.00 3969678.00 4262659.00 6148147.00 5492384.00 4123644.00 4824430.00 C 4591780.00 18037472.00 11043980.00 2319089.00 13406065.00 4313618.00 1880031.00 2650054.00 12498290.00 6257151.00 6092174.00 5204153.00 D 8631190.00 14710960.00 12327725.00 6077443.00 17042896.00 4402805.00 6679158.00 2692759.00 11576516.00 4919056.00 12115326.00 6487425.00 E 1924485.00 14907531.00 8085271.00 15933600.00 15514181.00 3640551.00 2007122.00 5003101.00 7578898.00 3828130.00 11360587.00 9806162.00 F 8247981.00 7175419.00 5009283.00 3848554.00 11186417.00 2455612.00 1560528.00 5908835.00 4792530.00 10205176.00 4759634.00 11988725.00 G 1859767.00 7036369.00 14041863.00 2402137.00 35596844.00 3387058.00 3024166.00 4388617.00 5140343.00 2080939.00 5961996.00 5407518.00 H 3281203.00 4007788.00 5758609.00 1352596.00 11713832.00 3730785.00 4369205.00 7298219.00 4940355.00 5129474.00 7377885.00 16086798.00 Load time: 04/27/2012 02:28:30 PM Read time: 04/27/2012 02:28:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2532 Comment: GBS plate 2 dil 2 + 98 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 49082300 cps Min counts: 49482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22366102.00 17271018.00 13747001.00 13993398.00 16389481.00 10134918.00 13537375.00 3571694.00 5344178.00 10434525.00 7281057.00 98965.00 B 19560622.00 12622185.00 38076044.00 14522480.00 14328192.00 12974675.00 19478912.00 12693597.00 25700706.00 6534159.00 3504424.00 473214.00 C 10661216.00 24468218.00 24224960.00 13354011.00 15922443.00 22197796.00 16258787.00 5751242.00 14710121.00 13931597.00 14145993.00 13547883.00 D 23655570.00 20901576.00 20580542.00 5302213.00 31658288.00 19258006.00 25275484.00 3857581.00 15987073.00 15060536.00 8155238.00 648909.00 E 18490600.00 23170588.00 18908396.00 19990670.00 24737610.00 6218004.00 13276504.00 12476548.00 16632087.00 4990245.00 4529255.00 129638.00 F 17871116.00 23395232.00 19003672.00 22399474.00 13408808.00 19049652.00 27775356.00 24550654.00 24539046.00 7078695.00 10039946.00 6125618.00 G 18155904.00 23779624.00 28112268.00 6763470.00 39047544.00 23917996.00 20964430.00 25189266.00 23894638.00 4722646.00 11213252.00 5617507.00 H 49082300.00 18436498.00 14827393.00 26243056.00 11901983.00 15976040.00 7226349.00 9355957.00 8361516.00 8092625.00 7492003.00 5514734.00 Load time: 04/27/2012 02:30:02 PM Read time: 04/27/2012 02:30:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2533 Comment: GBS plate 2 dil 2 + 98 2nd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 47996348 cps Min counts: 49954 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23071094.00 18163414.00 13917051.00 14100638.00 16676535.00 10237065.00 13591253.00 3592275.00 5341690.00 10501350.00 7249087.00 99909.00 B 19661850.00 12447943.00 38200204.00 14715020.00 14266140.00 13047963.00 19582124.00 12810685.00 25474438.00 6485585.00 3504306.00 470703.00 C 10691734.00 24522624.00 24445396.00 13434788.00 16055664.00 22211188.00 16290374.00 5743168.00 14660943.00 13889748.00 14016051.00 13489169.00 D 23407626.00 20955280.00 20814076.00 5269771.00 31800744.00 19330810.00 25155582.00 3868906.00 16075361.00 15139841.00 8105216.00 653722.00 E 18596538.00 23350226.00 18849712.00 20029098.00 25140530.00 6254711.00 13283210.00 12464279.00 16735193.00 4976767.00 4525898.00 128147.00 F 17843072.00 23268846.00 19269622.00 22516244.00 13396286.00 19055756.00 28056570.00 24448874.00 24325106.00 7166321.00 10177905.00 6096646.00 G 18231642.00 24787734.00 28462380.00 6792456.00 38876724.00 23913336.00 21070240.00 25434716.00 23519776.00 4704304.00 11237472.00 5708759.00 H 47996348.00 18453880.00 14727383.00 26849654.00 12016469.00 16058165.00 7213714.00 9437545.00 8420279.00 8142895.00 7531596.00 5543496.00 Load time: 04/27/2012 02:31:52 PM Read time: 04/27/2012 02:31:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2534 Comment: GBS plate 1 dil 52 + 95 3rd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36533408 cps Min counts: 616764 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2680877.00 1233528.00 10516634.00 11236279.00 10083079.00 13176090.00 9128402.00 4323242.00 1881538.00 11460311.00 3898585.00 4779048.00 B 3452673.00 7184621.00 7051605.00 5985998.00 8259859.00 4346100.00 4003106.00 4248563.00 6208084.00 5507396.00 4046588.00 4827713.00 C 4612787.00 18527200.00 11153399.00 2328100.00 13713085.00 4345793.00 1888620.00 2702592.00 12611314.00 6387456.00 6186490.00 5179566.00 D 8764254.00 14607097.00 12846938.00 6100885.00 17088208.00 4259257.00 6803933.00 2710551.00 11613504.00 4924448.00 12294047.00 6511525.00 E 1947926.00 15013598.00 7958533.00 15878362.00 15691994.00 3693796.00 2003321.00 5066800.00 7628492.00 3828388.00 11444753.00 9773709.00 F 8262753.00 7266727.00 4983497.00 3827213.00 11207775.00 2403497.00 1558160.00 5978366.00 4814750.00 11102803.00 4806014.00 12094914.00 G 1874773.00 7064175.00 14051408.00 2414157.00 36533408.00 3392109.00 3034689.00 4463606.00 5255389.00 2091852.00 6041299.00 5470749.00 H 3256674.00 4002663.00 5759463.00 1359372.00 11756403.00 3723278.00 4389018.00 7384255.00 4955758.00 5159970.00 7559572.00 16102479.00