Load time: 5/6/2011 16:06 Read time: 5/6/2011 16:06 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #1531 Comment: 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 69772008 cps Min counts: 67694 counts Well list: "r0(a1:h2,a3:f3,g3) = cps(f6(a1:h2,a3:f3,g3))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62953620 69772008 12468883 B 46537588 49234164 8462717 C 26495706 25917128 8032163 D 14173265 12572769 5366907 E 9047052 8677689 3759527 F 5074397 5205909 2615503 G 3397525 3453299 135389 H 1963234 2073459 Well list: "r1(a1:h2,a3:f3) = cps(f6(a1:h2,a3:f3) - avg(f6(g3)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62818231 69636619 12333494 B 46402199 49098775 8327328 C 26360317 25781739 7896774 D 14037876 12437380 5231518 E 8911663 8542300 3624138 F 4939008 5070520 2480114 G 3262136 3317910 H 1827845 1938070 Load time: 5/6/2011 16:07 Read time: 5/6/2011 16:07 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #1532 Comment: 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 73212208 cps Min counts: 69787 counts Well list: "r0(a1:h2,a3:f3,g3) = cps(f6(a1:h2,a3:f3,g3))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66145920 73212208 13184236 B 48917064 51187804 8850029 C 27751168 27050342 8441572 D 14622504 13107296 5675515 E 9489272 9047320 3928704 F 5313139 5480576 2715662 G 3551499 3631488 139575 H 2054092 2161313 Well list: "r1(a1:h2,a3:f3) = cps(f6(a1:h2,a3:f3) - avg(f6(g3)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66006345 73072633 13044661 "69,539,489" "13,044,661" B 48777489 51048229 8710454 "49,912,859" "8,710,454" C 27611593 26910767 8301997 "27,261,180" "8,301,997" D 14482929 12967721 5535940 "13,725,325" "5,535,940" E 9349697 8907745 3789129 "9,128,721" "3,789,129" F 5173564 5341001 2576087 "5,257,283" "2,576,087" G 3411924 3491913 "3,451,919" H 1914517 2021738 "1,968,128" Load time: 5/6/2011 16:10 Read time: 5/6/2011 16:10 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #1533 Comment: 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 74673832 cps Min counts: 72439 counts Well list: "r0(a1:h2,a3:f3,g3) = cps(f6(a1:h2,a3:f3,g3))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 67873672 74673832 13682130 B 50698284 52810216 9121440 C 28583706 28030792 8677543 D 15098542 13552497 5810417 E 9773523 9316805 4053451 F 5507179 5683604 2821625 G 3660787 3746707 144878 H 2123116 2202026 Well list: "r1(a1:h2,a3:f3) = cps(f6(a1:h2,a3:f3) - avg(f6(g3)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 67728794 74528954 13537252 B 50553406 52665338 8976562 C 28438828 27885914 8532665 D 14953664 13407619 5665539 E 9628645 9171927 3908573 F 5362301 5538726 2676747 G 3515909 3601829 H 1978238 2057148