Load time: 04/21/2011 04:08:12 PM Read time: 04/21/2011 04:08:12 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1490 Comment: 444 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 73473840 cps Min counts: 86405 counts Well list: r0(a11:h11,a10:f10,a1:h9,g10:h10) = cps(f2(a11:h11,a10:f10,a1:h9,g10:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3484524.00 1652221.00 2351421.00 3647999.00 4444717.00 3770758.00 3902531.00 4015722.00 2157602.00 4809181.00 73473840.00 B 3239488.00 3710655.00 892463.00 2385266.00 4125753.00 3968412.00 3305586.00 4556095.00 6570718.00 5327954.00 45899408.00 C 3133342.00 2719117.00 4130902.00 3455705.00 4441267.00 3842749.00 2996297.00 3519507.00 6689765.00 5582803.00 26874100.00 D 3857768.00 290422.00 2251666.00 4011741.00 3802241.00 4828116.00 7070961.00 3419416.00 5552460.00 5801949.00 15823969.00 E 3848090.00 2693744.00 3557211.00 4429187.00 2679569.00 182172.00 3454017.00 3768346.00 6491110.00 5664415.00 9503953.00 F 4025321.00 2033740.00 197965.00 3914609.00 4553258.00 3612589.00 4720323.00 2661055.00 6026079.00 4114385.00 5845430.00 G 3470011.00 3916661.00 5233756.00 2354049.00 5078103.00 4766157.00 4478298.00 4491321.00 6676035.00 173876.00 3371938.00 H 4351298.00 2454925.00 239655.00 647156.00 6492640.00 3064113.00 4499158.00 4331534.00 6537056.00 172811.00 2212016.00 Well list: r1(a11:h11,a10:f10,a1:h9) = cps(f2(a11:h11,a10:f10,a1:h9) - avg(f2(g10:h10))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3311180.50 1478877.50 2178077.50 3474655.50 4271373.50 3597414.50 3729187.50 3842378.50 1984258.50 4635837.50 73300496.50 B 3066144.50 3537311.50 719119.50 2211922.50 3952409.50 3795068.50 3132242.50 4382751.50 6397374.50 5154610.50 45726064.50 C 2959998.50 2545773.50 3957558.50 3282361.50 4267923.50 3669405.50 2822953.50 3346163.50 6516421.50 5409459.50 26700756.50 D 3684424.50 117078.50 2078322.50 3838397.50 3628897.50 4654772.50 6897617.50 3246072.50 5379116.50 5628605.50 15650625.50 E 3674746.50 2520400.50 3383867.50 4255843.50 2506225.50 8828.50 3280673.50 3595002.50 6317766.50 5491071.50 9330609.50 F 3851977.50 1860396.50 24621.50 3741265.50 4379914.50 3439245.50 4546979.50 2487711.50 5852735.50 3941041.50 5672086.50 G 3296667.50 3743317.50 5060412.50 2180705.50 4904759.50 4592813.50 4304954.50 4317977.50 6502691.50 3198594.50 H 4177954.50 2281581.50 66311.50 473812.50 6319296.50 2890769.50 4325814.50 4158190.50 6363712.50 2038672.50