Load time: 04/21/2011 04:02:23 PM Read time: 04/21/2011 04:02:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1487 Comment: 444 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 69969304 cps Min counts: 75445 counts Well list: r0(a11:h11,a10:f10,a1:h9,g10:h10) = cps(f2(a11:h11,a10:f10,a1:h9,g10:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3540263.00 1227944.00 2136497.00 3610448.00 3903248.00 3592240.00 3735000.00 3979601.00 2028201.00 4474747.00 69969304.00 B 3310743.00 3623269.00 755596.00 2448184.00 3628990.00 3982951.00 3176087.00 4315529.00 6596433.00 4649014.00 37424728.00 C 2986171.00 2731939.00 3953297.00 794489.00 4160926.00 3290999.00 2923642.00 3296970.00 5573286.00 4706100.00 22835662.00 D 3787610.00 272174.00 2191634.00 3969212.00 3619105.00 4540096.00 6272482.00 3021827.00 4923991.00 5432836.00 13031778.00 E 3326922.00 2361817.00 3661285.00 4622466.00 2472397.00 173229.00 3340143.00 3253439.00 5982221.00 5213775.00 7848049.00 F 3889785.00 1937426.00 196310.00 3898913.00 4338635.00 3523618.00 4189018.00 2188453.00 5283352.00 3853488.00 4417731.00 G 3440728.00 3867516.00 5445131.00 2369085.00 4183821.00 4305162.00 4160764.00 3738761.00 6334289.00 150890.00 2693138.00 H 4209980.00 2471860.00 227820.00 4536204.00 5690616.00 2985808.00 3997437.00 3483386.00 5487324.00 173020.00 1940194.00 Well list: r1(a11:h11,a10:f10,a1:h9) = cps(f2(a11:h11,a10:f10,a1:h9) - avg(f2(g10:h10))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3378308.00 1065989.00 1974542.00 3448493.00 3741293.00 3430285.00 3573045.00 3817646.00 1866246.00 4312792.00 69807349.00 B 3148788.00 3461314.00 593641.00 2286229.00 3467035.00 3820996.00 3014132.00 4153574.00 6434478.00 4487059.00 37262773.00 C 2824216.00 2569984.00 3791342.00 632534.00 3998971.00 3129044.00 2761687.00 3135015.00 5411331.00 4544145.00 22673707.00 D 3625655.00 110219.00 2029679.00 3807257.00 3457150.00 4378141.00 6110527.00 2859872.00 4762036.00 5270881.00 12869823.00 E 3164967.00 2199862.00 3499330.00 4460511.00 2310442.00 11274.00 3178188.00 3091484.00 5820266.00 5051820.00 7686094.00 F 3727830.00 1775471.00 34355.00 3736958.00 4176680.00 3361663.00 4027063.00 2026498.00 5121397.00 3691533.00 4255776.00 G 3278773.00 3705561.00 5283176.00 2207130.00 4021866.00 4143207.00 3998809.00 3576806.00 6172334.00 2531183.00 H 4048025.00 2309905.00 65865.00 4374249.00 5528661.00 2823853.00 3835482.00 3321431.00 5325369.00 1778239.00