Load time: 04/21/2011 04:06:15 PM Read time: 04/21/2011 04:06:15 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1489 Comment: 444 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 73469680 cps Min counts: 84255 counts Well list: r0(a11:h11,a10:f10,a1:h9,g10:h10) = cps(f2(a11:h11,a10:f10,a1:h9,g10:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3416158.00 1607694.00 2310398.00 3604001.00 4433917.00 3760277.00 3809184.00 4055497.00 2185845.00 4886555.00 73469680.00 B 3191743.00 3668477.00 880704.00 2364303.00 4050372.00 3945863.00 3306253.00 4576556.00 6632703.00 5386602.00 45713376.00 C 3125380.00 2683468.00 4070778.00 3399266.00 4410973.00 3795608.00 2968486.00 3534948.00 6579966.00 5585492.00 26722064.00 D 3847748.00 289489.00 2211457.00 3958914.00 3828686.00 4758295.00 7024179.00 3422875.00 5488657.00 5699538.00 15852454.00 E 3844871.00 2654843.00 3490956.00 4368541.00 2643541.00 180840.00 3415210.00 3701745.00 6370189.00 5614239.00 9610528.00 F 4050077.00 2026004.00 196881.00 3878355.00 4497614.00 3611441.00 4660974.00 2640656.00 5934768.00 4077540.00 5786408.00 G 3409949.00 3846785.00 5117518.00 2322655.00 4998240.00 4690066.00 4407646.00 4433009.00 6531523.00 171460.00 3373198.00 H 4297091.00 2431057.00 238304.00 640211.00 6391445.00 3005145.00 4478728.00 4278829.00 6497160.00 168510.00 2201746.00 Well list: r1(a11:h11,a10:f10,a1:h9) = cps(f2(a11:h11,a10:f10,a1:h9) - avg(f2(g10:h10))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3246173.00 1437709.00 2140413.00 3434016.00 4263932.00 3590292.00 3639199.00 3885512.00 2015860.00 4716570.00 73299695.00 B 3021758.00 3498492.00 710719.00 2194318.00 3880387.00 3775878.00 3136268.00 4406571.00 6462718.00 5216617.00 45543391.00 C 2955395.00 2513483.00 3900793.00 3229281.00 4240988.00 3625623.00 2798501.00 3364963.00 6409981.00 5415507.00 26552079.00 D 3677763.00 119504.00 2041472.00 3788929.00 3658701.00 4588310.00 6854194.00 3252890.00 5318672.00 5529553.00 15682469.00 E 3674886.00 2484858.00 3320971.00 4198556.00 2473556.00 10855.00 3245225.00 3531760.00 6200204.00 5444254.00 9440543.00 F 3880092.00 1856019.00 26896.00 3708370.00 4327629.00 3441456.00 4490989.00 2470671.00 5764783.00 3907555.00 5616423.00 G 3239964.00 3676800.00 4947533.00 2152670.00 4828255.00 4520081.00 4237661.00 4263024.00 6361538.00 3203213.00 H 4127106.00 2261072.00 68319.00 470226.00 6221460.00 2835160.00 4308743.00 4108844.00 6327175.00 2031761.00