Load time: 08/17/2012 03:24:04 PM Read time: 08/17/2012 03:24:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2800 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46354504 cps Min counts: 20134 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43209808.00 35006188.00 45729.00 B 46354504.00 32274656.00 40268.00 C 27388010.00 17301908.00 D 10701119.00 9656056.00 E 9609124.00 4374031.00 F 3996169.00 2605497.00 G 2038422.00 1553455.00 H 1213142.00 1189597.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43166809.50 34963189.50 B 46311505.50 32231657.50 C 27345011.50 17258909.50 D 10658120.50 9613057.50 E 9566125.50 4331032.50 F 3953170.50 2562498.50 G 1995423.50 1510456.50 H 1170143.50 1146598.50 Load time: 08/17/2012 03:24:45 PM Read time: 08/17/2012 03:24:45 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2801 Comment: standards2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46213352 cps Min counts: 21249 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43285444.00 34654944.00 49131.00 B 46213352.00 32005074.00 42498.00 C 27590590.00 17095170.00 D 10724248.00 9625939.00 E 9587413.00 4378888.00 F 4034668.00 2618099.00 G 2070393.00 1548032.00 H 1208853.00 1214163.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43239629.50 34609129.50 B 46167537.50 31959259.50 C 27544775.50 17049355.50 D 10678433.50 9580124.50 E 9541598.50 4333073.50 F 3988853.50 2572284.50 G 2024578.50 1502217.50 H 1163038.50 1168348.50 Load time: 08/17/2012 03:27:54 PM Read time: 08/17/2012 03:27:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2803 Comment: samples Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27504900 cps Min counts: 28174 counts Well list: r0(a1:a12,b1:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = cps(f2(a1:a12,b1:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19914332.00 13310204.00 15357504.00 12982685.00 19079232.00 13939570.00 7997945.00 15150829.00 10901054.00 10385779.00 12386864.00 12092118.00 B 16815800.00 13652989.00 18710990.00 18196156.00 13074508.00 16218312.00 8095798.00 18102062.00 13742415.00 12084754.00 14640319.00 23183432.00 C 18768928.00 19395542.00 21013084.00 7788362.00 24264524.00 18139308.00 17395194.00 12688118.00 21365224.00 18367624.00 3840431.00 17227048.00 D 10439147.00 15666645.00 5700610.00 9896400.00 25529616.00 17525408.00 14510767.00 19672592.00 23336158.00 13501143.00 18897236.00 15444102.00 E 16881450.00 27504900.00 21389194.00 11690779.00 14569769.00 18448794.00 19130064.00 9677475.00 22298210.00 15124894.00 10889538.00 1269057.00 F 18374122.00 21215888.00 10985313.00 18345162.00 21435516.00 19217126.00 14946815.00 19345996.00 11742515.00 15124480.00 19426992.00 63001.00 G 18065810.00 24380470.00 18803354.00 14131129.00 4871239.00 10965020.00 13984652.00 20757022.00 16208565.00 22680316.00 15368786.00 58730.00 H 17737058.00 18948588.00 18333906.00 9699461.00 21171086.00 11542449.00 980317.00 19597032.00 13238493.00 18831120.00 13620004.00 56349.00 Load time: 08/17/2012 03:29:26 PM Read time: 08/17/2012 03:29:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2804 Comment: samples2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27448522 cps Min counts: 28146 counts Well list: r0(a1:a12,b1:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = cps(f2(a1:a12,b1:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19741484.00 13278967.00 15305088.00 12895237.00 18925838.00 14016460.00 7998085.00 15154656.00 10900487.00 10375394.00 12391663.00 11986538.00 B 16707667.00 13650461.00 18805090.00 18247762.00 13102284.00 16279884.00 8029015.00 18141182.00 13841751.00 12004218.00 14778692.00 23226424.00 C 18642040.00 19394084.00 20851242.00 7775375.00 24294304.00 18213066.00 17198112.00 12755343.00 21275604.00 18315294.00 3847938.00 17084566.00 D 10317925.00 15686840.00 5720467.00 9893161.00 25497322.00 17568370.00 14628199.00 19592270.00 23084624.00 13440165.00 18913952.00 15637463.00 E 16845628.00 27448522.00 21403922.00 11626191.00 14524602.00 18453544.00 19053634.00 9730371.00 22302518.00 15204192.00 10853029.00 942203.00 F 18308494.00 21038272.00 10979330.00 18281984.00 21490628.00 19184356.00 15075520.00 19330152.00 11773756.00 15102406.00 19361290.00 63057.00 G 17882120.00 23940380.00 18832130.00 14045100.00 4850277.00 10979690.00 13844573.00 20820952.00 16083175.00 22664378.00 15529284.00 58843.00 H 17674400.00 18982746.00 18243504.00 9669651.00 21108616.00 11487050.00 980109.00 19551884.00 13253568.00 18777398.00 13577856.00 56292.00 Load time: 08/17/2012 03:32:50 PM Read time: 08/17/2012 03:32:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2805 Comment: samples2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27724604 cps Min counts: 26842 counts Well list: r0(a12:e12,a11:h11,a1:a9,b1:b9,c1:c9,d1:d9,e1:e10,a10:d10,f1:f10,g1:g10,h1:h10,f12:h12) = cps(f2(a12:e12,a11:h11,a1:a9,b1:b9,c1:c9,d1:d9,e1:e10,a10:d10,f1:f10,g1:g10,h1:h10,f12:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19738112.00 13163609.00 15442438.00 12987501.00 18985166.00 13987988.00 7945172.00 15149317.00 10876317.00 10359438.00 12457480.00 12162345.00 B 16662814.00 13644470.00 18828374.00 18210152.00 13072853.00 16183045.00 8041695.00 18297068.00 13865852.00 12077288.00 14667147.00 23075332.00 C 18591352.00 19467842.00 20939864.00 7799240.00 24343590.00 18213066.00 17289078.00 12714109.00 21370650.00 18563838.00 3864229.00 17275610.00 D 10340547.00 15650837.00 5749891.00 9890053.00 25460922.00 17609658.00 14587397.00 19855784.00 23228586.00 13595505.00 19027872.00 15620673.00 E 16686594.00 27724604.00 21531104.00 11633495.00 14511203.00 18311916.00 19087842.00 9794338.00 22302582.00 15327791.00 10853790.00 1341582.00 F 18233876.00 20919602.00 10966217.00 18402518.00 21355200.00 19126994.00 15088334.00 19438652.00 11787437.00 15478261.00 19510486.00 60393.00 G 17942518.00 24166490.00 18739436.00 14095745.00 4850527.00 11020491.00 13892083.00 20846412.00 16249842.00 22840882.00 15412888.00 58333.00 H 17616794.00 18813180.00 18249182.00 9694565.00 21219610.00 11551891.00 983548.00 19693714.00 13358146.00 18731364.00 13769722.00 53685.00 Well list: r1(a12:e12,a11:h11,a1:a9,b1:b9,c1:c9,d1:d9,e1:e10,a10:d10,f1:f10,g1:g10,h1:h10) = cps(f2(a12:e12,a11:h11,a1:a9,b1:b9,c1:c9,d1:d9,e1:e10,a10:d10,f1:f10,g1:g10,h1:h10) - avg(f2(f12:h12))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19680641.67 13106138.67 15384967.67 12930030.67 18927695.67 13930517.67 7887701.67 15091846.67 10818846.67 10301967.67 12400009.67 12104874.67 B 16605343.67 13586999.67 18770903.67 18152681.67 13015382.67 16125574.67 7984224.67 18239597.67 13808381.67 12019817.67 14609676.67 23017861.67 C 18533881.67 19410371.67 20882393.67 7741769.67 24286119.67 18155595.67 17231607.67 12656638.67 21313179.67 18506367.67 3806758.67 17218139.67 D 10283076.67 15593366.67 5692420.67 9832582.67 25403451.67 17552187.67 14529926.67 19798313.67 23171115.67 13538034.67 18970401.67 15563202.67 E 16629123.67 27667133.67 21473633.67 11576024.67 14453732.67 18254445.67 19030371.67 9736867.67 22245111.67 15270320.67 10796319.67 1284111.67 F 18176405.67 20862131.67 10908746.67 18345047.67 21297729.67 19069523.67 15030863.67 19381181.67 11729966.67 15420790.67 19453015.67 G 17885047.67 24109019.67 18681965.67 14038274.67 4793056.67 10963020.67 13834612.67 20788941.67 16192371.67 22783411.67 15355417.67 H 17559323.67 18755709.67 18191711.67 9637094.67 21162139.67 11494420.67 926077.67 19636243.67 13300675.67 18673893.67 13712251.67