Load time: 08/24/2012 03:30:33 PM Read time: 08/24/2012 03:30:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2813 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56457760 cps Min counts: 20098 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56457760.00 43787572.00 40631.00 B 40634180.00 30723964.00 40197.00 C 23781222.00 16870834.00 D 22748024.00 10714325.00 E 8003874.00 5397535.00 F 4261427.00 3341585.00 G 2299185.00 1910810.00 H 1362522.00 1296037.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56417346.00 43747158.00 B 40593766.00 30683550.00 C 23740808.00 16830420.00 D 22707610.00 10673911.00 E 7963460.00 5357121.00 F 4221013.00 3301171.00 G 2258771.00 1870396.00 H 1322108.00 1255623.00 Load time: 08/24/2012 03:33:57 PM Read time: 08/24/2012 03:33:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2814 Comment: plate 2 dilution 6+60 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95262144 cps Min counts: 20363 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56139612.00 95262144.00 60616208.00 66220204.00 76925016.00 37228140.00 71402448.00 8077867.00 16178123.00 43767020.00 23039912.00 40726.00 B 50051476.00 31987348.00 61946804.00 37709648.00 41237044.00 37504288.00 82822192.00 52748284.00 65994944.00 17987538.00 8922683.00 581146.00 C 25789808.00 64046164.00 84943440.00 43950912.00 53909124.00 55820604.00 53121856.00 17423704.00 44068512.00 42627016.00 49515200.00 63448752.00 D 65256664.00 84984472.00 58527664.00 11204438.00 94771128.00 56540648.00 78434024.00 10484812.00 54530800.00 36362836.00 36873824.00 963387.00 E 69136608.00 68493056.00 46438640.00 76818904.00 90363520.00 11957653.00 36738368.00 45806052.00 41339004.00 13300408.00 13682072.00 60550.00 F 61131772.00 64496544.00 50852116.00 95207072.00 24353734.00 33578180.00 90011528.00 75472976.00 62066508.00 25698706.00 39576720.00 20370286.00 G 75223616.00 89958176.00 60521420.00 66454424.00 86929496.00 52194860.00 67971608.00 45188092.00 66758756.00 11262408.00 47365268.00 18298186.00 H 53796724.00 58417688.00 41910412.00 84883832.00 70013936.00 52628504.00 32813672.00 41964712.00 32659718.00 29564940.00 37319136.00 33910764.00 Load time: 08/24/2012 03:35:40 PM Read time: 08/24/2012 03:35:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2815 Comment: plate 2 dilution 6+60 second Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 96060096 cps Min counts: 20004 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 55058292.00 94742120.00 59791680.00 65561752.00 76087240.00 37530292.00 71143232.00 8081122.00 15863910.00 43143280.00 22062328.00 40008.00 B 49197044.00 32073234.00 61413068.00 36700220.00 41859420.00 36953388.00 83105848.00 53126596.00 65490760.00 17975048.00 8948832.00 567313.00 C 24992366.00 63713520.00 83698008.00 44015392.00 53719820.00 55512364.00 52772384.00 17280640.00 44230424.00 43033672.00 48636316.00 63082668.00 D 65186876.00 83345656.00 58621632.00 11157620.00 96060096.00 56226880.00 78637200.00 10462775.00 54578860.00 35452524.00 36542388.00 953824.00 E 68884960.00 68266528.00 45259428.00 74747112.00 89940408.00 11798507.00 36552740.00 45171220.00 41626664.00 13341992.00 13626940.00 59246.00 F 61447684.00 63772308.00 49815048.00 94894272.00 24282000.00 33937976.00 89949808.00 74765880.00 62998980.00 25509682.00 38299228.00 20502202.00 G 74683656.00 90075408.00 59843912.00 65805316.00 88440128.00 51626400.00 67676256.00 47169008.00 66261584.00 11128954.00 46819516.00 18003728.00 H 53826652.00 58309876.00 41283884.00 84052448.00 70145264.00 52772384.00 31973344.00 41795228.00 31850598.00 28882006.00 35792840.00 33507242.00 Load time: 08/24/2012 03:39:01 PM Read time: 08/24/2012 03:39:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2816 Comment: plate 2 dilution 2+100 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22973310 cps Min counts: 17509 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8959893.00 12273481.00 8559965.00 9574358.00 10318234.00 6518143.00 8483601.00 1847563.00 3088201.00 7371794.00 4155635.00 35018.00 B 6689767.00 8927282.00 21532218.00 9174740.00 9542831.00 8111931.00 12055854.00 7383466.00 14607866.00 4606943.00 1818552.00 153436.00 C 3552673.00 14605165.00 15506215.00 8694220.00 9230515.00 14445207.00 10338256.00 3292185.00 9569695.00 8940976.00 9307807.00 7921095.00 D 9839760.00 11810098.00 13329380.00 2279741.00 18950278.00 15835707.00 15044986.00 2320607.00 9692575.00 8833486.00 4814404.00 179365.00 E 10770812.00 16081592.00 11835089.00 12292036.00 15787553.00 2619671.00 8455393.00 6545508.00 10781779.00 3090764.00 3242472.00 43088.00 F 5974950.00 13439746.00 12292602.00 13089357.00 8118840.00 11440851.00 17160846.00 13866879.00 15798509.00 4435941.00 6936664.00 2743256.00 G 12516147.00 14428593.00 17596670.00 4300474.00 22973310.00 11516715.00 13017139.00 12388747.00 15820788.00 3052464.00 7725707.00 3349224.00 H 6569969.00 11685213.00 10533276.00 20030820.00 7548299.00 8681785.00 4043478.00 5787314.00 5446906.00 5305119.00 4885740.00 3159206.00 Load time: 08/24/2012 03:40:36 PM Read time: 08/24/2012 03:40:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2817 Comment: plate 2 dilution 2+100 second Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23111174 cps Min counts: 17140 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9093506.00 12275466.00 8554081.00 9754046.00 10470742.00 6547801.00 8505815.00 1838466.00 3093078.00 7410637.00 4142645.00 34281.00 B 6752790.00 8901024.00 21831298.00 9175120.00 9591228.00 8116056.00 12265759.00 7382494.00 14648281.00 4606800.00 1811859.00 151981.00 C 3582892.00 14549166.00 15600372.00 8719162.00 9224673.00 14973278.00 10375545.00 3274589.00 9680806.00 9100337.00 9341709.00 7936305.00 D 10075323.00 11868204.00 13356910.00 2275536.00 19211302.00 15720031.00 14937018.00 2307319.00 9764271.00 8907558.00 4790414.00 177267.00 E 10854987.00 16135396.00 11848602.00 12454905.00 15809703.00 2623102.00 8414857.00 6491366.00 10758404.00 3072642.00 3283755.00 42351.00 F 5988424.00 13404790.00 12331414.00 13141781.00 8052524.00 11445332.00 17310590.00 13992359.00 15896130.00 4444236.00 6886818.00 2746692.00 G 11760414.00 14602824.00 17688430.00 4316190.00 23111174.00 11528018.00 13012455.00 12318541.00 15844070.00 3050290.00 7793338.00 3337811.00 H 6670139.00 11953425.00 10454494.00 20462688.00 7610361.00 8682594.00 4063163.00 5780344.00 5436451.00 5330439.00 4897951.00 3165201.00 Load time: 08/24/2012 03:43:22 PM Read time: 08/24/2012 03:43:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2818 Comment: plate 1 dilution 5+95 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25204762 cps Min counts: 68195 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 776915.00 136390.00 6769911.00 7965289.00 5765052.00 8881884.00 5369228.00 2476097.00 1058276.00 7821679.00 2298129.00 3190552.00 B 1845440.00 2922353.00 5179008.00 4443488.00 4663298.00 2499906.00 2657791.00 2352140.00 4642917.00 3723527.00 3784996.00 2896643.00 C 2491846.00 11404308.00 7433560.00 1544722.00 8393761.00 2045498.00 844837.00 1559068.00 9172196.00 4478959.00 4247514.00 3117861.00 D 4836308.00 9760220.00 7760063.00 4723612.00 11318745.00 2528839.00 4070413.00 1550114.00 9305582.00 3455999.00 7357499.00 4085220.00 E 946661.00 10430884.00 5981672.00 10529037.00 12005688.00 2160246.00 1359423.00 3140303.00 5537532.00 2691063.00 7085449.00 6129029.00 F 4705234.00 5042016.00 4317259.00 2475571.00 6196098.00 1271885.00 948117.00 3600196.00 3742356.00 6570115.00 3258895.00 7811197.00 G 872069.00 4909473.00 11356607.00 693346.00 25204762.00 2342045.00 2011939.00 2775034.00 4009216.00 1097566.00 3928328.00 3633823.00 H 1618105.00 862885.00 4604446.00 387437.00 7694390.00 2321043.00 2803043.00 4408216.00 3802616.00 2935499.00 4774258.00 6238997.00 Load time: 08/24/2012 03:45:16 PM Read time: 08/24/2012 03:45:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2819 Comment: plate 1 dilution 5+95 second Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25455072 cps Min counts: 66900 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 767976.00 133801.00 6668198.00 7833988.00 5757133.00 8881660.00 5241823.00 2442172.00 1044669.00 7877565.00 2322716.00 3177148.00 B 1828128.00 2906255.00 5132503.00 4394520.00 4607326.00 2467607.00 2641361.00 2331480.00 4626105.00 3751487.00 3775526.00 2854321.00 C 2460598.00 11423224.00 7332034.00 1527770.00 8181637.00 2025863.00 833025.00 1549375.00 9106975.00 4426065.00 4244037.00 3090905.00 D 4864756.00 9706297.00 7646431.00 4696335.00 11227890.00 2489447.00 4006823.00 1558826.00 9298060.00 3448793.00 7330958.00 4023453.00 E 920298.00 10374350.00 5922280.00 10403295.00 11889002.00 2140316.00 1361483.00 3131443.00 5571361.00 2696925.00 7044208.00 6142744.00 F 4642348.00 5031480.00 4256703.00 2456313.00 6154965.00 1257239.00 946095.00 3588112.00 3777632.00 6689450.00 3240877.00 7831000.00 G 848484.00 4883342.00 11150147.00 686864.00 25455072.00 2310380.00 1993090.00 2780283.00 4013771.00 1090082.00 3923257.00 3600353.00 H 1565544.00 853038.00 4514753.00 379065.00 7620206.00 2302234.00 2792540.00 4342098.00 3768886.00 2926934.00 4757641.00 6246145.00 Load time: 08/24/2012 03:45:16 PM Read time: 08/24/2012 03:45:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2819 Comment: plate 1 dilution 5+95 second Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25455072 cps Min counts: 66900 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 767976.00 133801.00 6668198.00 7833988.00 5757133.00 8881660.00 5241823.00 2442172.00 1044669.00 7877565.00 2322716.00 3177148.00 B 1828128.00 2906255.00 5132503.00 4394520.00 4607326.00 2467607.00 2641361.00 2331480.00 4626105.00 3751487.00 3775526.00 2854321.00 C 2460598.00 11423224.00 7332034.00 1527770.00 8181637.00 2025863.00 833025.00 1549375.00 9106975.00 4426065.00 4244037.00 3090905.00 D 4864756.00 9706297.00 7646431.00 4696335.00 11227890.00 2489447.00 4006823.00 1558826.00 9298060.00 3448793.00 7330958.00 4023453.00 E 920298.00 10374350.00 5922280.00 10403295.00 11889002.00 2140316.00 1361483.00 3131443.00 5571361.00 2696925.00 7044208.00 6142744.00 F 4642348.00 5031480.00 4256703.00 2456313.00 6154965.00 1257239.00 946095.00 3588112.00 3777632.00 6689450.00 3240877.00 7831000.00 G 848484.00 4883342.00 11150147.00 686864.00 25455072.00 2310380.00 1993090.00 2780283.00 4013771.00 1090082.00 3923257.00 3600353.00 H 1565544.00 853038.00 4514753.00 379065.00 7620206.00 2302234.00 2792540.00 4342098.00 3768886.00 2926934.00 4757641.00 6246145.00