Load time: Read time: Kinetic read cycle: 0 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2820 Comment: plate 1 dilution 5+95 second Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 0 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: well Load time: 08/24/2012 04:13:01 PM Read time: 08/24/2012 04:13:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2821 Comment: plate 1 no dilution Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 115250664 cps Min counts: 21027 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 42054.00 15183279.00 93762168.00 104206968.00 87575480.00 101853016.00 90221864.00 56983592.00 20522396.00 93392304.00 48806596.00 60635248.00 B 48445592.00 75848424.00 77603944.00 89411200.00 81887056.00 61995788.00 52895924.00 49029444.00 74688600.00 51883492.00 70516984.00 60845408.00 C 61097016.00 97445480.00 92000576.00 34373380.00 93120448.00 50020664.00 19991910.00 30263582.00 88637296.00 75375056.00 72198528.00 63872948.00 D 92764520.00 83036744.00 96750120.00 52275876.00 83510576.00 56384720.00 76223296.00 36234600.00 69333496.00 61098948.00 79835088.00 76762920.00 E 44920640.00 101488504.00 102078632.00 104635104.00 95572464.00 46357260.00 26439926.00 68389064.00 78216280.00 57015064.00 93518648.00 84284280.00 F 83355912.00 85915520.00 93090216.00 51078720.00 90535232.00 28448036.00 16821876.00 77687280.00 48202172.00 83526784.00 64496052.00 91732400.00 G 26098024.00 80342128.00 115250664.00 27555668.00 102536960.00 52332412.00 43449592.00 69070608.00 71921512.00 27481526.00 77516928.00 73398440.00 H 64360932.00 46431492.00 95921784.00 15260735.00 94060624.00 56212368.00 62796344.00 86534928.00 75279048.00 67959824.00 84224672.00 82555048.00 Load time: 08/24/2012 04:14:26 PM Read time: 08/24/2012 04:14:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2822 Comment: plate 1 no dilution read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 116387256 cps Min counts: 21197 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 42394.00 15290092.00 97276504.00 103814304.00 87764112.00 101657752.00 90762360.00 57987456.00 21509316.00 96203104.00 49268768.00 61370176.00 B 49789684.00 78020008.00 80617376.00 89299824.00 82859880.00 63428136.00 54511228.00 50874636.00 75930240.00 53279984.00 71260832.00 61629600.00 C 62412612.00 100194752.00 95210456.00 34568920.00 94732784.00 51450080.00 20215866.00 31776552.00 89402936.00 76897112.00 73144640.00 64391444.00 D 97365808.00 86204432.00 99306656.00 53274848.00 85852648.00 58418460.00 77659216.00 37284416.00 71454200.00 62808060.00 79934824.00 76548120.00 E 46574508.00 104873240.00 104337576.00 105615784.00 96862688.00 48360852.00 28501132.00 69760832.00 80577104.00 58895760.00 94359856.00 85460288.00 F 86899184.00 87550720.00 95652760.00 52895928.00 91649872.00 28928942.00 17351166.00 78685320.00 50186360.00 84105728.00 65699628.00 93189944.00 G 28431318.00 80927496.00 116387256.00 27912028.00 104309456.00 53534936.00 44867792.00 69716224.00 73193736.00 27788100.00 78372272.00 73611304.00 H 66327232.00 47732020.00 96005048.00 15501243.00 95566568.00 57995276.00 64342212.00 87500256.00 77307928.00 69766488.00 83945224.00 84239336.00 Load time: 08/24/2012 04:17:03 PM Read time: 08/24/2012 04:17:03 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2823 Comment: plate 1 no dilution read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51800180 cps Min counts: 16132 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51800180.00 39678384.00 32264.00 B 40199012.00 28623790.00 32944.00 C 20120244.00 16662502.00 D 22524032.00 9430640.00 E 7907252.00 5207019.00 F 4093229.00 3708868.00 G 2269236.00 1838476.00 H 1273190.00 1206715.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51767576.00 39645780.00 B 40166408.00 28591186.00 C 20087640.00 16629898.00 D 22491428.00 9398036.00 E 7874648.00 5174415.00 F 4060625.00 3676264.00 G 2236632.00 1805872.00 H 1240586.00 1174111.00