Load time: 04/27/2012 02:31:52 PM Read time: 04/27/2012 02:31:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2534 Comment: GBS plate 1 dil 52 + 95 3rd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36533408 cps Min counts: 616764 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2680877.00 1233528.00 10516634.00 11236279.00 10083079.00 13176090.00 9128402.00 4323242.00 1881538.00 11460311.00 3898585.00 4779048.00 B 3452673.00 7184621.00 7051605.00 5985998.00 8259859.00 4346100.00 4003106.00 4248563.00 6208084.00 5507396.00 4046588.00 4827713.00 C 4612787.00 18527200.00 11153399.00 2328100.00 13713085.00 4345793.00 1888620.00 2702592.00 12611314.00 6387456.00 6186490.00 5179566.00 D 8764254.00 14607097.00 12846938.00 6100885.00 17088208.00 4259257.00 6803933.00 2710551.00 11613504.00 4924448.00 12294047.00 6511525.00 E 1947926.00 15013598.00 7958533.00 15878362.00 15691994.00 3693796.00 2003321.00 5066800.00 7628492.00 3828388.00 11444753.00 9773709.00 F 8262753.00 7266727.00 4983497.00 3827213.00 11207775.00 2403497.00 1558160.00 5978366.00 4814750.00 11102803.00 4806014.00 12094914.00 G 1874773.00 7064175.00 14051408.00 2414157.00 36533408.00 3392109.00 3034689.00 4463606.00 5255389.00 2091852.00 6041299.00 5470749.00 H 3256674.00 4002663.00 5759463.00 1359372.00 11756403.00 3723278.00 4389018.00 7384255.00 4955758.00 5159970.00 7559572.00 16102479.00