Load time: 02/27/2013 05:26:30 PM Read time: 02/27/2013 05:26:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2952 Comment: plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 190186416 cps Min counts: 1810 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45835.00 66758916.00 121351152.00 115419376.00 165782096.00 46247068.00 142034048.00 129151008.00 4187.00 4225.00 B 78919.00 44007924.00 137267136.00 103709496.00 150491696.00 152876944.00 184866544.00 45889692.00 4055.00 3961.00 C 97706.00 26031156.00 60945048.00 75522408.00 161148496.00 164753328.00 127165552.00 17550544.00 3847.00 4017.00 D 103062.00 12612672.00 183514080.00 187577520.00 135154000.00 148592976.00 79373128.00 37284128.00 3659.00 3715.00 E 7280033.00 121630080.00 159022352.00 101848784.00 173999888.00 184456064.00 10325267.00 3621.00 3696.00 F 3350660.00 139213712.00 141048704.00 190186416.00 142832304.00 181304816.00 380506.00 3847.00 3791.00 G 1692115.00 160782960.00 130616104.00 154426176.00 166453568.00 161793056.00 2505991.00 4017.00 3678.00 H 957877.00 67652144.00 176234464.00 108197384.00 66439148.00 155708464.00 1177716.00 3715.00 3885.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66677535.50 121269771.50 115337995.50 165700715.50 46165687.50 141952667.50 129069627.50 -77193.50 -77155.50 B 43926543.50 137185755.50 103628115.50 150410315.50 152795563.50 184785163.50 45808311.50 -77325.50 -77419.50 C 25949775.50 60863667.50 75441027.50 161067115.50 164671947.50 127084171.50 17469163.50 -77533.50 -77363.50 D 12531291.50 183432699.50 187496139.50 135072619.50 148511595.50 79291747.50 37202747.50 -77721.50 -77665.50 E 7198652.50 121548699.50 158940971.50 101767403.50 173918507.50 184374683.50 10243886.50 -77759.50 -77684.50 F 3269279.50 139132331.50 140967323.50 190105035.50 142750923.50 181223435.50 299125.50 -77533.50 -77589.50 G 1610734.50 160701579.50 130534723.50 154344795.50 166372187.50 161711675.50 2424610.50 -77363.50 -77702.50 H 876496.50 67570763.50 176153083.50 108116003.50 66357767.50 155627083.50 1096335.50 -77665.50 -77495.50 Load time: 02/27/2013 05:28:40 PM Read time: 02/27/2013 05:28:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2953 Comment: plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 195474704 cps Min counts: 1641 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62622.00 77419688.00 162538512.00 81476656.00 93588800.00 165483808.00 12553016.00 195474704.00 3791.00 3621.00 B 73336.00 42525476.00 178824704.00 72690800.00 91998504.00 78061904.00 71098064.00 22400788.00 3885.00 3282.00 C 100516.00 27917592.00 137335440.00 3174831.00 22914290.00 139611168.00 15606231.00 127209504.00 3583.00 3508.00 D 102572.00 11875158.00 163817104.00 8978090.00 11360811.00 110631384.00 72933720.00 59953768.00 3734.00 3432.00 E 6879465.00 190514720.00 18031594.00 24749232.00 140464032.00 96083056.00 130830648.00 3715.00 3678.00 F 3520648.00 190655728.00 12495985.00 46032692.00 139919632.00 33320720.00 165327984.00 3866.00 3621.00 G 1729994.00 175777344.00 4176731.00 50045296.00 168677648.00 118155360.00 42575856.00 3546.00 3451.00 H 893519.00 161772736.00 2713574.00 7947560.00 62362820.00 76347832.00 74689368.00 3357.00 3376.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 77334926.50 162453750.50 81391894.50 93504038.50 165399046.50 12468254.50 195389942.50 -80970.50 -81140.50 B 42440714.50 178739942.50 72606038.50 91913742.50 77977142.50 71013302.50 22316026.50 -80876.50 -81479.50 C 27832830.50 137250678.50 3090069.50 22829528.50 139526406.50 15521469.50 127124742.50 -81178.50 -81253.50 D 11790396.50 163732342.50 8893328.50 11276049.50 110546622.50 72848958.50 59869006.50 -81027.50 -81329.50 E 6794703.50 190429958.50 17946832.50 24664470.50 140379270.50 95998294.50 130745886.50 -81046.50 -81083.50 F 3435886.50 190570966.50 12411223.50 45947930.50 139834870.50 33235958.50 165243222.50 -80895.50 -81140.50 G 1645232.50 175692582.50 4091969.50 49960534.50 168592886.50 118070598.50 42491094.50 -81215.50 -81310.50 H 808757.50 161687974.50 2628812.50 7862798.50 62278058.50 76263070.50 74604606.50 -81404.50 -81385.50 Load time: 02/27/2013 05:30:22 PM Read time: 02/27/2013 05:30:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2954 Comment: plate2bottom Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 209409200 cps Min counts: 1961 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 73543.00 86971536.00 184946208.00 97423080.00 111389016.00 167188400.00 13867596.00 209409200.00 4489.00 4357.00 B 85351.00 45047124.00 187787232.00 79986152.00 101805832.00 87409400.00 77544784.00 24939208.00 4508.00 4319.00 C 113135.00 30274070.00 150386224.00 3496257.00 24810826.00 146699744.00 17086820.00 142345456.00 4376.00 4243.00 D 116492.00 13170986.00 179602064.00 9865756.00 12544028.00 120195744.00 69305256.00 67009056.00 4545.00 4243.00 E 7374425.00 199482128.00 19706724.00 26953470.00 147966864.00 100566784.00 142028848.00 4451.00 4394.00 F 3688360.00 200392768.00 13602779.00 49638232.00 152677568.00 36364560.00 183205104.00 4602.00 4111.00 G 1837232.00 188796800.00 4484797.00 54011328.00 171174096.00 120963552.00 47517948.00 4564.00 4394.00 H 956934.00 179794320.00 2791772.00 8617327.00 66298888.00 74441088.00 82287176.00 3923.00 4281.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 86874405.75 184849077.75 97325949.75 111291885.75 167091269.75 13770465.75 209312069.75 -92641.25 -92773.25 B 44949993.75 187690101.75 79889021.75 101708701.75 87312269.75 77447653.75 24842077.75 -92622.25 -92811.25 C 30176939.75 150289093.75 3399126.75 24713695.75 146602613.75 16989689.75 142248325.75 -92754.25 -92887.25 D 13073855.75 179504933.75 9768625.75 12446897.75 120098613.75 69208125.75 66911925.75 -92585.25 -92887.25 E 7277294.75 199384997.75 19609593.75 26856339.75 147869733.75 100469653.75 141931717.75 -92679.25 -92736.25 F 3591229.75 200295637.75 13505648.75 49541101.75 152580437.75 36267429.75 183107973.75 -92528.25 -93019.25 G 1740101.75 188699669.75 4387666.75 53914197.75 171076965.75 120866421.75 47420817.75 -92566.25 -92736.25 H 859803.75 179697189.75 2694641.75 8520196.75 66201757.75 74343957.75 82190045.75 -93207.25 -92849.25 Load time: 02/27/2013 05:32:16 PM Read time: 02/27/2013 05:32:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2955 Comment: plate2_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 156481120 cps Min counts: 1442 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52719.00 66085664.00 140420608.00 76618128.00 82259136.00 127879080.00 10194608.00 156481120.00 3187.00 3225.00 B 60924.00 33523424.00 137818048.00 61396916.00 76022536.00 65113204.00 60675576.00 18174114.00 3244.00 3074.00 C 86237.00 22474162.00 114923208.00 2681102.00 19081158.00 109426416.00 12916720.00 102720840.00 3112.00 3225.00 D 86445.00 9815208.00 140204016.00 7605366.00 9533246.00 87436120.00 46457024.00 48605816.00 3149.00 2885.00 E 5652722.00 152292336.00 15387748.00 20423280.00 113772392.00 78548400.00 101905192.00 3112.00 3036.00 F 2796167.00 153603360.00 10566201.00 38432744.00 117877744.00 27111996.00 131527792.00 3168.00 3357.00 G 1469608.00 141945344.00 3508907.00 41565708.00 131747592.00 90985496.00 34120664.00 3206.00 2904.00 H 743339.00 139477600.00 2234522.00 6752944.00 50333844.00 59785360.00 63882440.00 2885.00 2980.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66014082.75 140349026.75 76546546.75 82187554.75 127807498.75 10123026.75 156409538.75 -68394.25 -68356.25 B 33451842.75 137746466.75 61325334.75 75950954.75 65041622.75 60603994.75 18102532.75 -68337.25 -68507.25 C 22402580.75 114851626.75 2609520.75 19009576.75 109354834.75 12845138.75 102649258.75 -68469.25 -68356.25 D 9743626.75 140132434.75 7533784.75 9461664.75 87364538.75 46385442.75 48534234.75 -68432.25 -68696.25 E 5581140.75 152220754.75 15316166.75 20351698.75 113700810.75 78476818.75 101833610.75 -68469.25 -68545.25 F 2724585.75 153531778.75 10494619.75 38361162.75 117806162.75 27040414.75 131456210.75 -68413.25 -68224.25 G 1398026.75 141873762.75 3437325.75 41494126.75 131676010.75 90913914.75 34049082.75 -68375.25 -68677.25 H 671757.75 139406018.75 2162940.75 6681362.75 50262262.75 59713778.75 63810858.75 -68696.25 -68601.25 Load time: 02/27/2013 05:33:50 PM Read time: 02/27/2013 05:33:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2956 Comment: plate1_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 148801872 cps Min counts: 1565 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39912.00 57130600.00 93902744.00 95109592.00 144415520.00 40796840.00 122321416.00 110329624.00 3564.00 3621.00 B 58001.00 34580664.00 89705112.00 64443540.00 132773240.00 110096944.00 125520984.00 38755668.00 3451.00 3338.00 C 79447.00 20231442.00 51384332.00 66541568.00 138943312.00 106435528.00 98105968.00 14453604.00 3451.00 3432.00 D 84785.00 9972574.00 140497216.00 145443200.00 125917872.00 114431288.00 71964968.00 31204330.00 3300.00 3149.00 E 5881087.00 82658688.00 119349064.00 93703440.00 139226240.00 148801872.00 8620183.00 3319.00 3168.00 F 2836948.00 113205600.00 117911912.00 144210688.00 133104928.00 139863920.00 292476.00 3414.00 3131.00 G 1409079.00 96709680.00 116099280.00 123954216.00 106732552.00 135826416.00 2152482.00 3432.00 3225.00 H 778913.00 64349068.00 143556848.00 99790016.00 57528780.00 139462480.00 1006994.00 3357.00 3338.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57065063.75 93837207.75 95044055.75 144349983.75 40731303.75 122255879.75 110264087.75 -61972.25 -61915.25 B 34515127.75 89639575.75 64378003.75 132707703.75 110031407.75 125455447.75 38690131.75 -62085.25 -62198.25 C 20165905.75 51318795.75 66476031.75 138877775.75 106369991.75 98040431.75 14388067.75 -62085.25 -62104.25 D 9907037.75 140431679.75 145377663.75 125852335.75 114365751.75 71899431.75 31138793.75 -62236.25 -62387.25 E 5815550.75 82593151.75 119283527.75 93637903.75 139160703.75 148736335.75 8554646.75 -62217.25 -62368.25 F 2771411.75 113140063.75 117846375.75 144145151.75 133039391.75 139798383.75 226939.75 -62122.25 -62405.25 G 1343542.75 96644143.75 116033743.75 123888679.75 106667015.75 135760879.75 2086945.75 -62104.25 -62311.25 H 713376.75 64283531.75 143491311.75 99724479.75 57463243.75 139396943.75 941457.75 -62179.25 -62198.25 Load time: 02/27/2013 05:33:50 PM Read time: 02/27/2013 05:33:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2956 Comment: plate1_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 148801872 cps Min counts: 1565 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39912.00 57130600.00 93902744.00 95109592.00 144415520.00 40796840.00 122321416.00 110329624.00 3564.00 3621.00 B 58001.00 34580664.00 89705112.00 64443540.00 132773240.00 110096944.00 125520984.00 38755668.00 3451.00 3338.00 C 79447.00 20231442.00 51384332.00 66541568.00 138943312.00 106435528.00 98105968.00 14453604.00 3451.00 3432.00 D 84785.00 9972574.00 140497216.00 145443200.00 125917872.00 114431288.00 71964968.00 31204330.00 3300.00 3149.00 E 5881087.00 82658688.00 119349064.00 93703440.00 139226240.00 148801872.00 8620183.00 3319.00 3168.00 F 2836948.00 113205600.00 117911912.00 144210688.00 133104928.00 139863920.00 292476.00 3414.00 3131.00 G 1409079.00 96709680.00 116099280.00 123954216.00 106732552.00 135826416.00 2152482.00 3432.00 3225.00 H 778913.00 64349068.00 143556848.00 99790016.00 57528780.00 139462480.00 1006994.00 3357.00 3338.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57065063.75 93837207.75 95044055.75 144349983.75 40731303.75 122255879.75 110264087.75 -61972.25 -61915.25 B 34515127.75 89639575.75 64378003.75 132707703.75 110031407.75 125455447.75 38690131.75 -62085.25 -62198.25 C 20165905.75 51318795.75 66476031.75 138877775.75 106369991.75 98040431.75 14388067.75 -62085.25 -62104.25 D 9907037.75 140431679.75 145377663.75 125852335.75 114365751.75 71899431.75 31138793.75 -62236.25 -62387.25 E 5815550.75 82593151.75 119283527.75 93637903.75 139160703.75 148736335.75 8554646.75 -62217.25 -62368.25 F 2771411.75 113140063.75 117846375.75 144145151.75 133039391.75 139798383.75 226939.75 -62122.25 -62405.25 G 1343542.75 96644143.75 116033743.75 123888679.75 106667015.75 135760879.75 2086945.75 -62104.25 -62311.25 H 713376.75 64283531.75 143491311.75 99724479.75 57463243.75 139396943.75 941457.75 -62179.25 -62198.25