Load time: 2/27/2013 17:26 Read time: 2/27/2013 17:26 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2952 Comment: plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 190186416 cps Min counts: 1810 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45835 66758916 121351152 115419376 165782096 46247068 142034048 129151008 4187 4225 B 78919 44007924 137267136 103709496 150491696 152876944 184866544 45889692 4055 3961 C 97706 26031156 60945048 75522408 161148496 164753328 127165552 17550544 3847 4017 D 103062 12612672 183514080 187577520 135154000 148592976 79373128 37284128 3659 3715 E 7280033 121630080 159022352 101848784 173999888 184456064 10325267 3621 3696 F 3350660 139213712 141048704 190186416 142832304 181304816 380506 3847 3791 G 1692115 160782960 130616104 154426176 166453568 161793056 2505991 4017 3678 H 957877 67652144 176234464 108197384 66439148 155708464 1177716 3715 3885 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A "66,677,536" "121,269,772" "115,337,996" "165,700,716" "46,165,688" "141,952,668" "129,069,628" B "43,926,544" "137,185,756" "103,628,116" "150,410,316" "152,795,564" "184,785,164" "45,808,312" C "25,949,776" "60,863,668" "75,441,028" "161,067,116" "164,671,948" "127,084,172" "17,469,164" D "12,531,292" "183,432,700" "187,496,140" "135,072,620" "148,511,596" "79,291,748" "37,202,748" E "7,198,653" "121,548,700" "158,940,972" "101,767,404" "173,918,508" "184,374,684" "10,243,887" F "3,269,280" "139,132,332" "140,967,324" "190,105,036" "142,750,924" "181,223,436" "299,126" G "1,610,735" "160,701,580" "130,534,724" "154,344,796" "166,372,188" "161,711,676" "2,424,611" H "876,497" "67,570,764" "176,153,084" "108,116,004" "66,357,768" "155,627,084" "1,096,336" Load time: 2/27/2013 17:28 Read time: 2/27/2013 17:28 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2953 Comment: plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 195474704 cps Min counts: 1641 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62622 77419688 162538512 81476656 93588800 165483808 12553016 195474704 3791 3621 B 73336 42525476 178824704 72690800 91998504 78061904 71098064 22400788 3885 3282 C 100516 27917592 137335440 3174831 22914290 139611168 15606231 127209504 3583 3508 D 102572 11875158 163817104 8978090 11360811 110631384 72933720 59953768 3734 3432 E 6879465 190514720 18031594 24749232 140464032 96083056 130830648 3715 3678 F 3520648 190655728 12495985 46032692 139919632 33320720 165327984 3866 3621 G 1729994 175777344 4176731 50045296 168677648 118155360 42575856 3546 3451 H 893519 161772736 2713574 7947560 62362820 76347832 74689368 3357 3376 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A "77,334,927" "162,453,751" "81,391,895" "93,504,039" "165,399,047" "12,468,255" "195,389,943" B "42,440,715" "178,739,943" "72,606,039" "91,913,743" "77,977,143" "71,013,303" "22,316,027" C "27,832,831" "137,250,679" "3,090,070" "22,829,529" "139,526,407" "15,521,470" "127,124,743" D "11,790,397" "163,732,343" "8,893,329" "11,276,050" "110,546,623" "72,848,959" "59,869,007" E "6,794,704" "190,429,959" "17,946,833" "24,664,471" "140,379,271" "95,998,295" "130,745,887" F "3,435,887" "190,570,967" "12,411,224" "45,947,931" "139,834,871" "33,235,959" "165,243,223" G "1,645,233" "175,692,583" "4,091,970" "49,960,535" "168,592,887" "118,070,599" "42,491,095" H "808,758" "161,687,975" "2,628,813" "7,862,799" "62,278,059" "76,263,071" "74,604,607" Load time: 2/27/2013 17:30 Read time: 2/27/2013 17:30 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2954 Comment: plate2bottom Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 209409200 cps Min counts: 1961 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 73543 86971536 184946208 97423080 111389016 167188400 13867596 209409200 4489 4357 B 85351 45047124 187787232 79986152 101805832 87409400 77544784 24939208 4508 4319 C 113135 30274070 150386224 3496257 24810826 146699744 17086820 142345456 4376 4243 D 116492 13170986 179602064 9865756 12544028 120195744 69305256 67009056 4545 4243 E 7374425 199482128 19706724 26953470 147966864 100566784 142028848 4451 4394 F 3688360 200392768 13602779 49638232 152677568 36364560 183205104 4602 4111 G 1837232 188796800 4484797 54011328 171174096 120963552 47517948 4564 4394 H 956934 179794320 2791772 8617327 66298888 74441088 82287176 3923 4281 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 86874405.75 184849077.8 97325949.75 111291885.8 167091269.8 13770465.75 209312069.8 -92641.25 -92773.25 B 44949993.75 187690101.8 79889021.75 101708701.8 87312269.75 77447653.75 24842077.75 -92622.25 -92811.25 C 30176939.75 150289093.8 3399126.75 24713695.75 146602613.8 16989689.75 142248325.8 -92754.25 -92887.25 D 13073855.75 179504933.8 9768625.75 12446897.75 120098613.8 69208125.75 66911925.75 -92585.25 -92887.25 E 7277294.75 199384997.8 19609593.75 26856339.75 147869733.8 100469653.8 141931717.8 -92679.25 -92736.25 F 3591229.75 200295637.8 13505648.75 49541101.75 152580437.8 36267429.75 183107973.8 -92528.25 -93019.25 G 1740101.75 188699669.8 4387666.75 53914197.75 171076965.8 120866421.8 47420817.75 -92566.25 -92736.25 H 859803.75 179697189.8 2694641.75 8520196.75 66201757.75 74343957.75 82190045.75 -93207.25 -92849.25 Load time: 2/27/2013 17:32 Read time: 2/27/2013 17:32 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2955 Comment: plate2_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 156481120 cps Min counts: 1442 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52719 66085664 140420608 76618128 82259136 127879080 10194608 156481120 3187 3225 B 60924 33523424 137818048 61396916 76022536 65113204 60675576 18174114 3244 3074 C 86237 22474162 114923208 2681102 19081158 109426416 12916720 102720840 3112 3225 D 86445 9815208 140204016 7605366 9533246 87436120 46457024 48605816 3149 2885 E 5652722 152292336 15387748 20423280 113772392 78548400 101905192 3112 3036 F 2796167 153603360 10566201 38432744 117877744 27111996 131527792 3168 3357 G 1469608 141945344 3508907 41565708 131747592 90985496 34120664 3206 2904 H 743339 139477600 2234522 6752944 50333844 59785360 63882440 2885 2980 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66014082.75 140349026.8 76546546.75 82187554.75 127807498.8 10123026.75 156409538.8 -68394.25 -68356.25 B 33451842.75 137746466.8 61325334.75 75950954.75 65041622.75 60603994.75 18102532.75 -68337.25 -68507.25 C 22402580.75 114851626.8 2609520.75 19009576.75 109354834.8 12845138.75 102649258.8 -68469.25 -68356.25 D 9743626.75 140132434.8 7533784.75 9461664.75 87364538.75 46385442.75 48534234.75 -68432.25 -68696.25 E 5581140.75 152220754.8 15316166.75 20351698.75 113700810.8 78476818.75 101833610.8 -68469.25 -68545.25 F 2724585.75 153531778.8 10494619.75 38361162.75 117806162.8 27040414.75 131456210.8 -68413.25 -68224.25 G 1398026.75 141873762.8 3437325.75 41494126.75 131676010.8 90913914.75 34049082.75 -68375.25 -68677.25 H 671757.75 139406018.8 2162940.75 6681362.75 50262262.75 59713778.75 63810858.75 -68696.25 -68601.25 Load time: 2/27/2013 17:33 Read time: 2/27/2013 17:33 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2956 Comment: plate1_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 148801872 cps Min counts: 1565 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39912 57130600 93902744 95109592 144415520 40796840 122321416 110329624 3564 3621 B 58001 34580664 89705112 64443540 132773240 110096944 125520984 38755668 3451 3338 C 79447 20231442 51384332 66541568 138943312 106435528 98105968 14453604 3451 3432 D 84785 9972574 140497216 145443200 125917872 114431288 71964968 31204330 3300 3149 E 5881087 82658688 119349064 93703440 139226240 148801872 8620183 3319 3168 F 2836948 113205600 117911912 144210688 133104928 139863920 292476 3414 3131 G 1409079 96709680 116099280 123954216 106732552 135826416 2152482 3432 3225 H 778913 64349068 143556848 99790016 57528780 139462480 1006994 3357 3338 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57065063.75 93837207.75 95044055.75 144349983.8 40731303.75 122255879.8 110264087.8 -61972.25 -61915.25 B 34515127.75 89639575.75 64378003.75 132707703.8 110031407.8 125455447.8 38690131.75 -62085.25 -62198.25 C 20165905.75 51318795.75 66476031.75 138877775.8 106369991.8 98040431.75 14388067.75 -62085.25 -62104.25 D 9907037.75 140431679.8 145377663.8 125852335.8 114365751.8 71899431.75 31138793.75 -62236.25 -62387.25 E 5815550.75 82593151.75 119283527.8 93637903.75 139160703.8 148736335.8 8554646.75 -62217.25 -62368.25 F 2771411.75 113140063.8 117846375.8 144145151.8 133039391.8 139798383.8 226939.75 -62122.25 -62405.25 G 1343542.75 96644143.75 116033743.8 123888679.8 106667015.8 135760879.8 2086945.75 -62104.25 -62311.25 H 713376.75 64283531.75 143491311.8 99724479.75 57463243.75 139396943.8 941457.75 -62179.25 -62198.25 Load time: 2/27/2013 17:33 Read time: 2/27/2013 17:33 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2956 Comment: plate1_3mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 148801872 cps Min counts: 1565 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39912 57130600 93902744 95109592 144415520 40796840 122321416 110329624 3564 3621 B 58001 34580664 89705112 64443540 132773240 110096944 125520984 38755668 3451 3338 C 79447 20231442 51384332 66541568 138943312 106435528 98105968 14453604 3451 3432 D 84785 9972574 140497216 145443200 125917872 114431288 71964968 31204330 3300 3149 E 5881087 82658688 119349064 93703440 139226240 148801872 8620183 3319 3168 F 2836948 113205600 117911912 144210688 133104928 139863920 292476 3414 3131 G 1409079 96709680 116099280 123954216 106732552 135826416 2152482 3432 3225 H 778913 64349068 143556848 99790016 57528780 139462480 1006994 3357 3338 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57065063.75 93837207.75 95044055.75 144349983.8 40731303.75 122255879.8 110264087.8 -61972.25 -61915.25 B 34515127.75 89639575.75 64378003.75 132707703.8 110031407.8 125455447.8 38690131.75 -62085.25 -62198.25 C 20165905.75 51318795.75 66476031.75 138877775.8 106369991.8 98040431.75 14388067.75 -62085.25 -62104.25 D 9907037.75 140431679.8 145377663.8 125852335.8 114365751.8 71899431.75 31138793.75 -62236.25 -62387.25 E 5815550.75 82593151.75 119283527.8 93637903.75 139160703.8 148736335.8 8554646.75 -62217.25 -62368.25 F 2771411.75 113140063.8 117846375.8 144145151.8 133039391.8 139798383.8 226939.75 -62122.25 -62405.25 G 1343542.75 96644143.75 116033743.8 123888679.8 106667015.8 135760879.8 2086945.75 -62104.25 -62311.25 H 713376.75 64283531.75 143491311.8 99724479.75 57463243.75 139396943.8 941457.75 -62179.25 -62198.25