Load time: 3/6/2013 11:20 Read time: 3/6/2013 11:20 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2958 Comment: plate1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 105311376 cps Min counts: 1632 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50650 65647508 56822692 17032250 25227938 7824187 23679486 29636384 3398 3322 B 54274 31943586 27257498 16002236 7974778 12090382 34507712 16663768 3511 3530 C 54614 20259086 10619449 5103754 21552714 33586220 5772637 3245210 3303 3473 D 55180 9690199 57325248 10072147 105311376 4108521 3508280 8797335 3492 3303 E 6160113 23328712 9014872 5110662 44617800 2431469 30967238 3416 3530 F 2063186 7736696 12844601 19099686 9512086 19117014 10299672 3662 3265 G 1325661 22589270 8849428 17810390 8401226 6409880 10585171 3492 3492 H 834414 14531284 17258710 20321220 13027439 15777401 11861155 3454 3549 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65593828.5 56769012.5 16978570.5 25174258.5 7770507.5 23625806.5 29582704.5 -50281.5 -50357.5 B 31889906.5 27203818.5 15948556.5 7921098.5 12036702.5 34454032.5 16610088.5 -50168.5 -50149.5 C 20205406.5 10565769.5 5050074.5 21499034.5 33532540.5 5718957.5 3191530.5 -50376.5 -50206.5 D 9636519.5 57271568.5 10018467.5 105257696.5 4054841.5 3454600.5 8743655.5 -50187.5 -50376.5 E 6106433.5 23275032.5 8961192.5 5056982.5 44564120.5 2377789.5 30913558.5 -50263.5 -50149.5 F 2009506.5 7683016.5 12790921.5 19046006.5 9458406.5 19063334.5 10245992.5 -50017.5 -50414.5 G 1271981.5 22535590.5 8795748.5 17756710.5 8347546.5 6356200.5 10531491.5 -50187.5 -50187.5 H 780734.5 14477604.5 17205030.5 20267540.5 12973759.5 15723721.5 11807475.5 -50225.5 -50130.5 Load time: 3/6/2013 11:23 Read time: 3/6/2013 11:23 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2959 Comment: plate2_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 100937096 cps Min counts: 1529 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 98713 66255000 59255056 41031388 100937096 47827360 21602026 87788384 3284 3228 B 39587 37678836 81009184 18729760 80816992 27229660 27625204 7240951 3511 3133 C 49498 21274018 18192934 27462144 41671324 36830320 11322123 26243674 3530 3171 D 50725 10397504 10504015 25978460 27474342 31297260 41333408 8409558 3398 3058 E 6181668 3112053 60716016 17705462 48649440 49065568 44379144 3228 3435 F 2074031 16948774 42514700 42018228 27096740 29661848 60033452 3492 3379 G 1355375 10860618 29012136 55326908 51660820 77941400 39417888 3265 3152 H 607706 17508538 19406664 42260732 14797632 78303680 38791812 3209 3284 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66195369.25 59195425.25 40971757.25 100877465.3 47767729.25 21542395.25 87728753.25 -56346.75 -56402.75 B 37619205.25 80949553.25 18670129.25 80757361.25 27170029.25 27565573.25 7181320.25 -56119.75 -56497.75 C 21214387.25 18133303.25 27402513.25 41611693.25 36770689.25 11262492.25 26184043.25 -56100.75 -56459.75 D 10337873.25 10444384.25 25918829.25 27414711.25 31237629.25 41273777.25 8349927.25 -56232.75 -56572.75 E 6122037.25 3052422.25 60656385.25 17645831.25 48589809.25 49005937.25 44319513.25 -56402.75 -56195.75 F 2014400.25 16889143.25 42455069.25 41958597.25 27037109.25 29602217.25 59973821.25 -56138.75 -56251.75 G 1295744.25 10800987.25 28952505.25 55267277.25 51601189.25 77881769.25 39358257.25 -56365.75 -56478.75 H 548075.25 17448907.25 19347033.25 42201101.25 14738001.25 78244049.25 38732181.25 -56421.75 -56346.75 Load time: 3/6/2013 11:25 Read time: 3/6/2013 11:25 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2960 Comment: plate1_middle Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59106948 cps Min counts: 1170 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27411 38422872 31281686 9516509 13047244 4478300 13199586 16917180 2435 2491 B 29091 17287880 15086929 9009105 4430445 6726260 19639312 9562516 2510 2529 C 28411 10797770 6025054 2890284 12008890 18708416 3274122 1850020 2340 2454 D 28638 5168036 30861070 5701462 59106948 2335206 2000905 5055814 2605 2435 E 3322813 13049688 5032848 2872862 25612918 1415011 16878960 2718 2680 F 1116718 4398935 7286703 10717759 5387018 10975791 5755489 2699 2605 G 736606 12435173 4949302 9698987 4800906 3669872 5943067 2491 2435 H 457946 8432468 9807550 11456284 7500323 8916112 6855121 2605 2718 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38394484.25 31253298.25 9488121.25 13018856.25 4449912.25 13171198.25 16888792.25 -25952.75 -25896.75 B 17259492.25 15058541.25 8980717.25 4402057.25 6697872.25 19610924.25 9534128.25 -25877.75 -25858.75 C 10769382.25 5996666.25 2861896.25 11980502.25 18680028.25 3245734.25 1821632.25 -26047.75 -25933.75 D 5139648.25 30832682.25 5673074.25 59078560.25 2306818.25 1972517.25 5027426.25 -25782.75 -25952.75 E 3294425.25 13021300.25 5004460.25 2844474.25 25584530.25 1386623.25 16850572.25 -25669.75 -25707.75 F 1088330.25 4370547.25 7258315.25 10689371.25 5358630.25 10947403.25 5727101.25 -25688.75 -25782.75 G 708218.25 12406785.25 4920914.25 9670599.25 4772518.25 3641484.25 5914679.25 -25896.75 -25952.75 H 429558.25 8404080.25 9779162.25 11427896.25 7471935.25 8887724.25 6826733.25 -25782.75 -25669.75 Load time: 3/6/2013 11:28 Read time: 3/6/2013 11:28 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2962 Comment: plate1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59821664 cps Min counts: 1170 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26863 38963828 32507614 9674220 13154964 4476550 13435399 17019804 2340 2473 B 28902 17190030 15533262 9036194 4584003 6794148 20126730 9549747 2454 2416 C 27580 10530335 6002613 2876370 12267482 19081492 3273243 1845612 2454 2397 D 30771 5103185 31500362 5713781 59821664 2322490 2033617 5038315 2624 2586 E 3337804 12991609 5074084 2918718 25302150 1413218 17253202 2359 2642 F 1123079 4591207 7363909 10741880 5489243 11222297 5783301 2661 2605 G 731320 12555329 5146134 10037982 4823950 3665506 5952926 2642 2642 H 450243 8603057 10126572 11638880 7572356 8881129 7044924 2586 2435 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38935299 32479085 9645691 13126435 4448021 13406870 16991275 -26189 -26056 B 17161501 15504733 9007665 4555474 6765619 20098201 9521218 -26075 -26113 C 10501806 5974084 2847841 12238953 19052963 3244714 1817083 -26075 -26132 D 5074656 31471833 5685252 59793135 2293961 2005088 5009786 -25905 -25943 E 3309275 12963080 5045555 2890189 25273621 1384689 17224673 -26170 -25887 F 1094550 4562678 7335380 10713351 5460714 11193768 5754772 -25868 -25924 G 702791 12526800 5117605 10009453 4795421 3636977 5924397 -25887 -25887 H 421714 8574528 10098043 11610351 7543827 8852600 7016395 -25943 -26094 Load time: 3/6/2013 11:30 Read time: 3/6/2013 11:30 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2963 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55090788 cps Min counts: 1151 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53236 38181168 32354050 33036632 55090788 26695882 12057370 49642972 2642 2529 B 20916 20954342 42315776 15134441 44046464 15386750 15181836 4090870 2624 2322 C 25881 11301568 9681647 22265286 23536268 20716924 6389610 14438893 2454 2416 D 25938 5437151 5544672 20800070 15113349 17828378 23212390 4610839 2378 2491 E 3365754 1742958 50608276 9854057 27590412 27281056 26013068 2510 2378 F 1139655 9045877 34313692 23293434 15117605 16577671 33630192 2416 2359 G 731169 6059402 23384660 31480740 29064808 44540440 22628076 2454 2510 H 327214 9576632 16249400 23877056 8537601 44126140 22160636 2454 2303 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38149675.25 32322557.25 33005139.25 55059295.25 26664389.25 12025877.25 49611479.25 -28850.75 -28963.75 B 20922849.25 42284283.25 15102948.25 44014971.25 15355257.25 15150343.25 4059377.25 -28868.75 -29170.75 C 11270075.25 9650154.25 22233793.25 23504775.25 20685431.25 6358117.25 14407400.25 -29038.75 -29076.75 D 5405658.25 5513179.25 20768577.25 15081856.25 17796885.25 23180897.25 4579346.25 -29114.75 -29001.75 E 3334261.25 1711465.25 50576783.25 9822564.25 27558919.25 27249563.25 25981575.25 -28982.75 -29114.75 F 1108162.25 9014384.25 34282199.25 23261941.25 15086112.25 16546178.25 33598699.25 -29076.75 -29133.75 G 699676.25 6027909.25 23353167.25 31449247.25 29033315.25 44508947.25 22596583.25 -29038.75 -28982.75 H 295721.25 9545139.25 16217907.25 23845563.25 8506108.25 44094647.25 22129143.25 -29038.75 -29189.75 Load time: 3/6/2013 11:30 Read time: 3/6/2013 11:30 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2963 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55090788 cps Min counts: 1151 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53236 38181168 32354050 33036632 55090788 26695882 12057370 49642972 2642 2529 B 20916 20954342 42315776 15134441 44046464 15386750 15181836 4090870 2624 2322 C 25881 11301568 9681647 22265286 23536268 20716924 6389610 14438893 2454 2416 D 25938 5437151 5544672 20800070 15113349 17828378 23212390 4610839 2378 2491 E 3365754 1742958 50608276 9854057 27590412 27281056 26013068 2510 2378 F 1139655 9045877 34313692 23293434 15117605 16577671 33630192 2416 2359 G 731169 6059402 23384660 31480740 29064808 44540440 22628076 2454 2510 H 327214 9576632 16249400 23877056 8537601 44126140 22160636 2454 2303 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38149675.25 32322557.25 33005139.25 55059295.25 26664389.25 12025877.25 49611479.25 -28850.75 -28963.75 B 20922849.25 42284283.25 15102948.25 44014971.25 15355257.25 15150343.25 4059377.25 -28868.75 -29170.75 C 11270075.25 9650154.25 22233793.25 23504775.25 20685431.25 6358117.25 14407400.25 -29038.75 -29076.75 D 5405658.25 5513179.25 20768577.25 15081856.25 17796885.25 23180897.25 4579346.25 -29114.75 -29001.75 E 3334261.25 1711465.25 50576783.25 9822564.25 27558919.25 27249563.25 25981575.25 -28982.75 -29114.75 F 1108162.25 9014384.25 34282199.25 23261941.25 15086112.25 16546178.25 33598699.25 -29076.75 -29133.75 G 699676.25 6027909.25 23353167.25 31449247.25 29033315.25 44508947.25 22596583.25 -29038.75 -28982.75 H 295721.25 9545139.25 16217907.25 23845563.25 8506108.25 44094647.25 22129143.25 -29038.75 -29189.75 Load time: 3/6/2013 11:32 Read time: 3/6/2013 11:32 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2964 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 107576464 cps Min counts: 1755 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 105491 74438168 62952260 37324544 107576464 51710568 22820404 93950336 3756 3794 B 40248 39781292 82633240 17134492 84908952 29639908 28285796 7845256 4172 3624 C 48592 21834924 18770774 25428542 44886652 39755524 11974060 27771352 4058 3718 D 49064 10486607 10637521 23628980 29353998 33723028 44094936 8725857 3794 3605 E 6285355 3342252 56144000 18776424 52320076 51969840 48360092 3681 3605 F 2135347 17500494 38477752 45428840 29023570 31440422 63672800 3662 3567 G 1361416 11634818 26180760 59018968 55497652 85475864 42551432 3794 3511 H 630813 18660652 18046914 45387512 16035452 83827376 41590404 3718 3662 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74377319.25 62891411.25 37263695.25 107515615.3 51649719.25 22759555.25 93889487.25 -57092.75 -57054.75 B 39720443.25 82572391.25 17073643.25 84848103.25 29579059.25 28224947.25 7784407.25 -56676.75 -57224.75 C 21774075.25 18709925.25 25367693.25 44825803.25 39694675.25 11913211.25 27710503.25 -56790.75 -57130.75 D 10425758.25 10576672.25 23568131.25 29293149.25 33662179.25 44034087.25 8665008.25 -57054.75 -57243.75 E 6224506.25 3281403.25 56083151.25 18715575.25 52259227.25 51908991.25 48299243.25 -57167.75 -57243.75 F 2074498.25 17439645.25 38416903.25 45367991.25 28962721.25 31379573.25 63611951.25 -57186.75 -57281.75 G 1300567.25 11573969.25 26119911.25 58958119.25 55436803.25 85415015.25 42490583.25 -57054.75 -57337.75 H 569964.25 18599803.25 17986065.25 45326663.25 15974603.25 83766527.25 41529555.25 -57130.75 -57186.75 Load time: 3/6/2013 11:34 Read time: 3/6/2013 11:34 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2965 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112392448 cps Min counts: 1859 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52424 74378744 62503944 18361242 30334190 8606687 25779860 31746862 3737 3907 B 55067 32194418 28402580 17258554 8924250 12897304 37862408 18078556 3888 3888 C 54935 20660576 11529224 5426246 23323434 35342380 6247698 3513237 3718 3870 D 55860 10101992 59592988 10892885 112392448 4366131 3821296 9566714 4115 3832 E 6504700 24507438 9631863 5532093 47138104 2741969 34363712 3926 4002 F 2110418 8520329 14005720 20164310 10467400 20740264 10983679 3945 4058 G 1381314 24105720 9720654 18826620 9025974 6875382 11358613 4134 3794 H 861693 15998561 18574942 22058352 14176554 16761330 13301238 3813 4096 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74324172.5 62449372.5 18306670.5 30279618.5 8552115.5 25725288.5 31692290.5 -50834.5 -50664.5 B 32139846.5 28348008.5 17203982.5 8869678.5 12842732.5 37807836.5 18023984.5 -50683.5 -50683.5 C 20606004.5 11474652.5 5371674.5 23268862.5 35287808.5 6193126.5 3458665.5 -50853.5 -50701.5 D 10047420.5 59538416.5 10838313.5 112337876.5 4311559.5 3766724.5 9512142.5 -50456.5 -50739.5 E 6450128.5 24452866.5 9577291.5 5477521.5 47083532.5 2687397.5 34309140.5 -50645.5 -50569.5 F 2055846.5 8465757.5 13951148.5 20109738.5 10412828.5 20685692.5 10929107.5 -50626.5 -50513.5 G 1326742.5 24051148.5 9666082.5 18772048.5 8971402.5 6820810.5 11304041.5 -50437.5 -50777.5 H 807121.5 15943989.5 18520370.5 22003780.5 14121982.5 16706758.5 13246666.5 -50758.5 -50475.5 Load time: 3/6/2013 11:35 Read time: 3/6/2013 11:35 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2966 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112123552 cps Min counts: 1783 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49574 75188032 63610988 18620254 29890598 8607377 25788200 31639566 3851 3700 B 52839 31438630 28562360 17305950 8904468 12794973 38163828 18039460 4021 3832 C 52556 19691978 11471309 5443911 23255256 35810660 6230431 3504972 3907 3756 D 52613 9537405 60017604 10913748 112123552 4395722 3823353 9483236 4002 3567 E 6348153 24490900 9775080 5575504 47212656 2741209 34479244 3983 3870 F 2053776 8663464 13878672 20164414 10516172 20740318 11013812 4039 3907 G 1343595 24134686 9750734 18846030 9085917 6854381 11434551 3945 3832 H 851782 15986654 18785548 22204608 14116305 16994480 13181629 3907 3851 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 75136136.5 63559092.5 18568358.5 29838702.5 8555481.5 25736304.5 31587670.5 -48044.5 -48195.5 B 31386734.5 28510464.5 17254054.5 8852572.5 12743077.5 38111932.5 17987564.5 -47874.5 -48063.5 C 19640082.5 11419413.5 5392015.5 23203360.5 35758764.5 6178535.5 3453076.5 -47988.5 -48139.5 D 9485509.5 59965708.5 10861852.5 112071656.5 4343826.5 3771457.5 9431340.5 -47893.5 -48328.5 E 6296257.5 24439004.5 9723184.5 5523608.5 47160760.5 2689313.5 34427348.5 -47912.5 -48025.5 F 2001880.5 8611568.5 13826776.5 20112518.5 10464276.5 20688422.5 10961916.5 -47856.5 -47988.5 G 1291699.5 24082790.5 9698838.5 18794134.5 9034021.5 6802485.5 11382655.5 -47950.5 -48063.5 H 799886.5 15934758.5 18733652.5 22152712.5 14064409.5 16942584.5 13129733.5 -47988.5 -48044.5 Load time: 3/6/2013 11:37 Read time: 3/6/2013 11:37 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2967 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 99505696 cps Min counts: 1736 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103131 71134736 58635424 34896736 99505696 49957608 22685432 84113696 3983 3700 B 36397 37507584 78461008 16336359 78328272 28951740 28368088 7405797 4134 3530 C 45590 22476662 17284210 24059596 42236956 38531624 11771289 25504950 3870 3605 D 46553 10851214 9810923 22435966 27494956 32692608 44387552 8371891 3888 3643 E 6441186 3075653 54432292 17921474 48751892 52051352 45779288 3718 3643 F 2139761 16283311 36253572 42782860 27891458 30716782 59180680 3737 3643 G 1382654 11409344 25295942 57099632 52951732 79181696 39460528 3681 3662 H 623167 17441324 16940754 42823140 15466153 76398176 39620464 3662 3473 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71076818.25 58577506.25 34838818.25 99447778.25 49899690.25 22627514.25 84055778.25 -53934.75 -54217.75 B 37449666.25 78403090.25 16278441.25 78270354.25 28893822.25 28310170.25 7347879.25 -53783.75 -54387.75 C 22418744.25 17226292.25 24001678.25 42179038.25 38473706.25 11713371.25 25447032.25 -54047.75 -54312.75 D 10793296.25 9753005.25 22378048.25 27437038.25 32634690.25 44329634.25 8313973.25 -54029.75 -54274.75 E 6383268.25 3017735.25 54374374.25 17863556.25 48693974.25 51993434.25 45721370.25 -54199.75 -54274.75 F 2081843.25 16225393.25 36195654.25 42724942.25 27833540.25 30658864.25 59122762.25 -54180.75 -54274.75 G 1324736.25 11351426.25 25238024.25 57041714.25 52893814.25 79123778.25 39402610.25 -54236.75 -54255.75 H 565249.25 17383406.25 16882836.25 42765222.25 15408235.25 76340258.25 39562546.25 -54255.75 -54444.75 Load time: 3/6/2013 11:37 Read time: 3/6/2013 11:37 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2967 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 99505696 cps Min counts: 1736 counts Well list: "r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103131 71134736 58635424 34896736 99505696 49957608 22685432 84113696 3983 3700 B 36397 37507584 78461008 16336359 78328272 28951740 28368088 7405797 4134 3530 C 45590 22476662 17284210 24059596 42236956 38531624 11771289 25504950 3870 3605 D 46553 10851214 9810923 22435966 27494956 32692608 44387552 8371891 3888 3643 E 6441186 3075653 54432292 17921474 48751892 52051352 45779288 3718 3643 F 2139761 16283311 36253572 42782860 27891458 30716782 59180680 3737 3643 G 1382654 11409344 25295942 57099632 52951732 79181696 39460528 3681 3662 H 623167 17441324 16940754 42823140 15466153 76398176 39620464 3662 3473 Well list: "r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1)))" Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71076818.25 58577506.25 34838818.25 99447778.25 49899690.25 22627514.25 84055778.25 -53934.75 -54217.75 B 37449666.25 78403090.25 16278441.25 78270354.25 28893822.25 28310170.25 7347879.25 -53783.75 -54387.75 C 22418744.25 17226292.25 24001678.25 42179038.25 38473706.25 11713371.25 25447032.25 -54047.75 -54312.75 D 10793296.25 9753005.25 22378048.25 27437038.25 32634690.25 44329634.25 8313973.25 -54029.75 -54274.75 E 6383268.25 3017735.25 54374374.25 17863556.25 48693974.25 51993434.25 45721370.25 -54199.75 -54274.75 F 2081843.25 16225393.25 36195654.25 42724942.25 27833540.25 30658864.25 59122762.25 -54180.75 -54274.75 G 1324736.25 11351426.25 25238024.25 57041714.25 52893814.25 79123778.25 39402610.25 -54236.75 -54255.75 H 565249.25 17383406.25 16882836.25 42765222.25 15408235.25 76340258.25 39562546.25 -54255.75 -54444.75 Load time: 3/6/2013 18:07 Read time: 3/6/2013 18:07 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2969 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 90349960 cps Min counts: 29267 counts Well list: "r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35561 72621400 64886456 20028346 19956918 8805101 16520625 40081092 B 40584 43353044 30275332 18364322 9049661 12871267 24249436 18934502 C 32519 20940836 11319309 14935047 21574068 31966386 6228347 3633134 D 10385843 90349960 31675582 43363176 4528248 2961490 21159434 E 6348192 33808420 10455889 5904490 51667408 2706496 63260172 F 2342661 8868482 48068872 20866236 18789786 21737602 33954672 G 1593868 26583002 10593739 20910850 15841072 7197842 23205954 H 691098 18268718 56343952 19362006 28016696 21664478 13415200 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72585178.67 64850234.67 19992124.67 19920696.67 8768879.67 16484403.67 40044870.67 B 43316822.67 30239110.67 18328100.67 9013439.67 12835045.67 24213214.67 18898280.67 C 20904614.67 11283087.67 14898825.67 21537846.67 31930164.67 6192125.67 3596912.67 D 10349621.67 90313738.67 31639360.67 43326954.67 4492026.67 2925268.67 21123212.67 E 6311970.67 33772198.67 10419667.67 5868268.67 51631186.67 2670274.67 63223950.67 F 2306439.67 8832260.67 48032650.67 20830014.67 18753564.67 21701380.67 33918450.67 G 1557646.67 26546780.67 10557517.67 20874628.67 15804850.67 7161620.67 23169732.67 H 654876.67 18232496.67 56307730.67 19325784.67 27980474.67 21628256.67 13378978.67 Load time: 3/6/2013 18:08 Read time: 3/6/2013 18:08 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2970 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 86936544 cps Min counts: 32750 counts Well list: "r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43793 71872816 50929452 21539576 53873876 49659904 58384040 15922685 B 236962 30488900 76127848 20659876 20548538 43149540 42747168 30372058 C 36389 21895498 19811578 17677418 44542004 20111032 43384396 9343207 D 11110378 11063955 20722448 42693660 31323680 33127050 45723228 E 7003244 3386807 56367448 86936544 19288054 84679456 49778912 F 2262468 26612382 49190724 47436288 44809180 79966656 21556492 G 1655297 12487101 34689284 33135492 49521720 61924840 57834508 H 707384 36415680 25643448 27825238 39925748 17477358 60640232 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71767101.33 50823737.33 21433861.33 53768161.33 49554189.33 58278325.33 15816970.33 B 30383185.33 76022133.33 20554161.33 20442823.33 43043825.33 42641453.33 30266343.33 C 21789783.33 19705863.33 17571703.33 44436289.33 20005317.33 43278681.33 9237492.33 D 11004663.33 10958240.33 20616733.33 42587945.33 31217965.33 33021335.33 45617513.33 E 6897529.33 3281092.33 56261733.33 86830829.33 19182339.33 84573741.33 49673197.33 F 2156753.33 26506667.33 49085009.33 47330573.33 44703465.33 79860941.33 21450777.33 G 1549582.33 12381386.33 34583569.33 33029777.33 49416005.33 61819125.33 57728793.33 H 601669.33 36309965.33 25537733.33 27719523.33 39820033.33 17371643.33 60534517.33 Load time: 3/6/2013 18:08 Read time: 3/6/2013 18:08 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2970 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 86936544 cps Min counts: 32750 counts Well list: "r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43793 71872816 50929452 21539576 53873876 49659904 58384040 15922685 B 236962 30488900 76127848 20659876 20548538 43149540 42747168 30372058 C 36389 21895498 19811578 17677418 44542004 20111032 43384396 9343207 D 11110378 11063955 20722448 42693660 31323680 33127050 45723228 E 7003244 3386807 56367448 86936544 19288054 84679456 49778912 F 2262468 26612382 49190724 47436288 44809180 79966656 21556492 G 1655297 12487101 34689284 33135492 49521720 61924840 57834508 H 707384 36415680 25643448 27825238 39925748 17477358 60640232 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71767101.33 50823737.33 21433861.33 53768161.33 49554189.33 58278325.33 15816970.33 B 30383185.33 76022133.33 20554161.33 20442823.33 43043825.33 42641453.33 30266343.33 C 21789783.33 19705863.33 17571703.33 44436289.33 20005317.33 43278681.33 9237492.33 D 11004663.33 10958240.33 20616733.33 42587945.33 31217965.33 33021335.33 45617513.33 E 6897529.33 3281092.33 56261733.33 86830829.33 19182339.33 84573741.33 49673197.33 F 2156753.33 26506667.33 49085009.33 47330573.33 44703465.33 79860941.33 21450777.33 G 1549582.33 12381386.33 34583569.33 33029777.33 49416005.33 61819125.33 57728793.33 H 601669.33 36309965.33 25537733.33 27719523.33 39820033.33 17371643.33 60534517.33