Load time: 09/04/2012 02:03:03 PM Read time: 09/04/2012 02:03:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2883 Comment: adapters 0.5 uM 1mm 10 ul Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 179176320 cps Min counts: 773701 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53593404.00 23687026.00 23056534.00 19929298.00 22057978.00 59358412.00 28941042.00 46691204.00 31165834.00 38700152.00 157265936.00 117248768.00 B 35644248.00 31648492.00 27549634.00 21351316.00 16090683.00 26492168.00 20312768.00 53902184.00 37871764.00 38869496.00 162276352.00 135946928.00 C 35458676.00 28302654.00 26493804.00 16977556.00 26470870.00 25536294.00 30263724.00 63990372.00 33241868.00 55218224.00 167522176.00 134076392.00 D 29386342.00 29319746.00 36206204.00 31419082.00 26885722.00 25049166.00 26608060.00 59482992.00 41491548.00 41731008.00 179176320.00 117535536.00 E 49331864.00 21289510.00 23275090.00 15234566.00 23634350.00 22962378.00 23428026.00 41264064.00 40290640.00 34955644.00 131418672.00 162508128.00 F 19378138.00 12655287.00 17073242.00 5326294.00 8765931.00 7570113.00 7340619.00 23179698.00 81412232.00 29191094.00 71615128.00 65423184.00 G 49191976.00 25578760.00 19731890.00 19507956.00 28547962.00 17193282.00 18699184.00 53064468.00 64057128.00 44897532.00 111444168.00 133659472.00 H 50757804.00 39241900.00 36270868.00 26298568.00 25905406.00 22685924.00 36906184.00 62843780.00 69839968.00 48126220.00 129195664.00 105151024.00 Load time: 09/04/2012 02:04:51 PM Read time: 09/04/2012 02:04:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2884 Comment: adapters 0.5 uM middle 10 ul Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95859504 cps Min counts: 773701 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27211382.00 12593983.00 12746270.00 11469002.00 13160690.00 32957674.00 15285523.00 25948238.00 17241444.00 20661454.00 82850696.00 60776832.00 B 18562174.00 16903520.00 14416003.00 11319332.00 8868071.00 14731806.00 10663547.00 29035206.00 20409000.00 20820806.00 84827288.00 70963528.00 C 18511058.00 14649630.00 13759947.00 8999874.00 14178323.00 13726917.00 17175458.00 35681896.00 18155860.00 30136764.00 88231408.00 70314096.00 D 15359931.00 15418148.00 19086672.00 17012460.00 15266401.00 13445477.00 14564033.00 34421296.00 21838754.00 22853316.00 95859504.00 61877284.00 E 27069526.00 11302366.00 12548371.00 8095412.00 13191724.00 12739575.00 12789907.00 23324276.00 21892144.00 18256150.00 69415760.00 84523328.00 F 10361510.00 6695581.00 9172020.00 2843687.00 4810201.00 4125282.00 4234252.00 12502706.00 43837004.00 15832827.00 41748576.00 36822576.00 G 27587748.00 13900088.00 10593626.00 10572027.00 16382186.00 10132364.00 11065094.00 29151718.00 35638172.00 23770570.00 59957316.00 70119096.00 H 27552284.00 20804570.00 19332044.00 14017570.00 14718745.00 13766312.00 21074084.00 36900200.00 40435728.00 27287968.00 69297640.00 56077900.00