Load time: 08/31/2012 02:57:32 PM Read time: 08/31/2012 02:57:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2871 Comment: standards background Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 20399946 cps Min counts: 42589 counts Well list: r0(a2:h3,a1:h1) = cps(f2(a2:h3,a1:h1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 94739.00 20399946.00 20370630.00 B 101106.00 9722646.00 12959550.00 C 101465.00 5122077.00 6669567.00 D 100615.00 2955121.00 3253078.00 E 101862.00 1989935.00 2048412.00 F 100804.00 1165324.00 1298136.00 G 101635.00 1382313.00 85178.00 H 101352.00 604958.00 676248.00 Well list: r1(a2:h3) = cps(f2(a2:h3) - avg(f2(a1:h1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20299498.75 20270182.75 B 9622198.75 12859102.75 C 5021629.75 6569119.75 D 2854673.75 3152630.75 E 1889487.75 1947964.75 F 1064876.75 1197688.75 G 1281865.75 -15269.25 H 504510.75 575800.75 Load time: 08/31/2012 02:58:32 PM Read time: 08/31/2012 02:58:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2872 Comment: standards background bottom Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40694448 cps Min counts: 82429 counts Well list: r0(a2:h3,a1:h1) = cps(f2(a2:h3,a1:h1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 194749.00 40280800.00 40694448.00 B 201703.00 19077528.00 24970002.00 C 218273.00 9833555.00 12927274.00 D 201400.00 5847516.00 6246098.00 E 201155.00 3916347.00 3950126.00 F 198302.00 2094613.00 2402769.00 G 198339.00 2501453.00 164858.00 H 198094.00 1119220.00 1354745.00 Well list: r1(a2:h3) = cps(f2(a2:h3) - avg(f2(a1:h1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40079298.13 40492946.13 B 18876026.13 24768500.13 C 9632053.13 12725772.13 D 5646014.13 6044596.13 E 3714845.13 3748624.13 F 1893111.13 2201267.13 G 2299951.13 -36643.88 H 917718.13 1153243.13 Load time: 08/31/2012 03:00:06 PM Read time: 08/31/2012 03:00:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2873 Comment: standards background bottom 2nd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 44760720 cps Min counts: 82788 counts Well list: r0(a2:h3,a1:h1) = cps(f2(a2:h3,a1:h1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 196129.00 44760720.00 41363020.00 B 201022.00 20597956.00 24443198.00 C 222544.00 9950311.00 13006309.00 D 199794.00 5919950.00 6218895.00 E 201098.00 3898949.00 3964596.00 F 198490.00 2098234.00 2434187.00 G 199530.00 3932623.00 165576.00 H 199133.00 1085021.00 1175471.00 Well list: r1(a2:h3) = cps(f2(a2:h3) - avg(f2(a1:h1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44558502.50 41160802.50 B 20395738.50 24240980.50 C 9748093.50 12804091.50 D 5717732.50 6016677.50 E 3696731.50 3762378.50 F 1896016.50 2231969.50 G 3730405.50 -36641.50 H 882803.50 973253.50 Load time: 08/31/2012 03:01:50 PM Read time: 08/31/2012 03:01:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2874 Comment: adapters 5 uM middle of well Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 126082984 cps Min counts: 773935 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44759684.00 14809011.00 12814091.00 24220824.00 30606778.00 36223424.00 56494504.00 28528282.00 79289328.00 27089372.00 69881280.00 53830736.00 B 45952964.00 16822302.00 16484315.00 20535322.00 44207940.00 75562632.00 76376192.00 33853212.00 88641200.00 26387514.00 43829404.00 59004248.00 C 55385136.00 14991250.00 18455634.00 18542122.00 55079652.00 102862200.00 121927568.00 48665068.00 76192728.00 29626468.00 52557484.00 38252648.00 D 49841912.00 17708002.00 17615258.00 27646666.00 70140944.00 110112288.00 78760824.00 42824864.00 78491624.00 37868572.00 58399184.00 44268884.00 E 63723024.00 11450728.00 19493166.00 21447026.00 100726912.00 126082984.00 102910072.00 32650812.00 78963312.00 33830128.00 60886568.00 46886124.00 F 31868466.00 11382891.00 9075225.00 13615863.00 42042964.00 54694684.00 48429068.00 27724012.00 45930056.00 17647514.00 31897886.00 32947576.00 G 73741832.00 22251666.00 22836684.00 38957384.00 107280920.00 96150576.00 74914600.00 39683288.00 107904632.00 49324768.00 51528692.00 38989964.00 H 82708360.00 22503154.00 29924302.00 45346088.00 62582704.00 28955974.00 72468552.00 33635316.00 89335952.00 46043456.00 56916208.00 49124708.00 Load time: 08/31/2012 03:03:41 PM Read time: 08/31/2012 03:03:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2875 Comment: adapters 5 uM bottom of well Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 242483712 cps Min counts: 773935 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 95727200.00 30269682.00 27706348.00 47942468.00 59793348.00 72190736.00 111178424.00 56520916.00 156908448.00 52720740.00 140284432.00 108289640.00 B 94422648.00 33740312.00 33213110.00 39786108.00 86629040.00 148622192.00 147253552.00 67787368.00 173695536.00 52237152.00 86704744.00 118414760.00 C 115431744.00 30159744.00 36823024.00 36740160.00 109859080.00 202330704.00 229463712.00 94598072.00 151472816.00 59028560.00 104024952.00 75336832.00 D 102465904.00 34900936.00 35162732.00 54419316.00 138444992.00 216231360.00 152987904.00 84421624.00 155277712.00 74028912.00 114655368.00 86694064.00 E 131271136.00 22533884.00 38792968.00 42438800.00 198348304.00 242483712.00 200724944.00 63625060.00 155443008.00 66089560.00 121090096.00 92858112.00 F 65357320.00 22191056.00 17476632.00 26123690.00 81578496.00 106423808.00 92913856.00 53691812.00 89893176.00 34163144.00 62884752.00 65261424.00 G 148158432.00 43663236.00 44861908.00 74531504.00 197598800.00 181914064.00 144528416.00 76525720.00 209590880.00 96824208.00 102427928.00 77891256.00 H 170440288.00 44826052.00 58906800.00 88876352.00 120299568.00 55322976.00 138286672.00 64548392.00 173174720.00 90019688.00 109728240.00 99101768.00 Load time: 08/31/2012 03:15:56 PM Read time: 08/31/2012 03:15:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2876 Comment: adapters 5 uM bottom of well Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41683600 cps Min counts: 79793 counts Well list: r0(a2:h3,a1:h1) = cps(f2(a2:h3,a1:h1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 179652.00 41683600.00 38891412.00 B 189364.00 18779776.00 25156926.00 C 195713.00 9803373.00 12890413.00 D 187777.00 5717273.00 6213103.00 E 191197.00 3851809.00 4036018.00 F 187588.00 2143760.00 2337228.00 G 188231.00 2325418.00 159586.00 H 189742.00 1137756.00 1166666.00 Well list: r1(a2:h3) = cps(f2(a2:h3) - avg(f2(a1:h1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 41494942.00 38702754.00 B 18591118.00 24968268.00 C 9614715.00 12701755.00 D 5528615.00 6024445.00 E 3663151.00 3847360.00 F 1955102.00 2148570.00 G 2136760.00 -29072.00 H 949098.00 978008.00 Load time: 08/31/2012 03:16:48 PM Read time: 08/31/2012 03:16:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2877 Comment: standards 1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41325916 cps Min counts: 80171 counts Well list: r0(a2:h3,a1:h1) = cps(f2(a2:h3,a1:h1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 180238.00 41325916.00 39284856.00 B 187267.00 18975884.00 23857434.00 C 199926.00 9616335.00 12725661.00 D 189515.00 5776899.00 6283349.00 E 191216.00 4050880.00 3971666.00 F 188401.00 2131954.00 2376278.00 G 189837.00 2302843.00 160342.00 H 189742.00 1077085.00 1181101.00 Well list: r1(a2:h3) = cps(f2(a2:h3) - avg(f2(a1:h1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 41136398.25 39095338.25 B 18786366.25 23667916.25 C 9426817.25 12536143.25 D 5587381.25 6093831.25 E 3861362.25 3782148.25 F 1942436.25 2186760.25 G 2113325.25 -29175.75 H 887567.25 991583.25 Load time: 08/31/2012 03:18:14 PM Read time: 08/31/2012 03:18:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2878 Comment: adapters 0.5 uM 1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2162864 cps Min counts: 384265 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1230700.00 1140761.00 1213412.00 1346526.00 1231041.00 1312572.00 1527917.00 1366555.00 1556921.00 1527653.00 1520643.00 1558809.00 B 1205835.00 1313460.00 1302312.00 1224881.00 1132598.00 1412979.00 1262406.00 1478847.00 1427793.00 1540350.00 1555158.00 1701261.00 C 1273894.00 1192306.00 1133203.00 1244116.00 1453963.00 1427018.00 1489749.00 1607396.00 1545810.00 1729502.00 1928840.00 1692149.00 D 1211295.00 1334207.00 1548583.00 1683200.00 1556198.00 1294754.00 1585499.00 1535570.00 1296190.00 1951897.00 2162864.00 1521021.00 E 1086513.00 1000107.00 1268906.00 1243624.00 1580996.00 1578704.00 1714965.00 1102706.00 1715565.00 2028636.00 2111004.00 1742697.00 F 768531.00 1081941.00 951641.00 872812.00 875589.00 1182726.00 1189566.00 1292638.00 957348.00 1612339.00 2100428.00 1547891.00 G 1157161.00 1458724.00 1252637.00 1499518.00 1361018.00 1501597.00 1364042.00 1533926.00 1517790.00 2000109.00 1988711.00 1651155.00 H 1249935.00 1270417.00 1245835.00 1531866.00 1008402.00 1789128.00 1500028.00 1727559.00 1674830.00 1839101.00 1837998.00 1617652.00 Load time: 08/31/2012 03:20:53 PM Read time: 08/31/2012 03:20:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2879 Comment: adapters 0.5 uM bottom Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2126904 cps Min counts: 380033 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1223690.00 1127572.00 1196293.00 1315746.00 1205873.00 1290219.00 1500066.00 1342256.00 1539046.00 1510156.00 1514804.00 1552890.00 B 1188603.00 1293942.00 1276029.00 1203737.00 1105087.00 1376852.00 1229926.00 1451185.00 1397013.00 1519169.00 1544828.00 1694175.00 C 1250521.00 1167157.00 1117406.00 1215339.00 1410013.00 1389021.00 1450240.00 1563000.00 1520265.00 1696823.00 1909049.00 1663222.00 D 1196444.00 1306960.00 1504676.00 1642987.00 1525725.00 1254980.00 1544918.00 1490052.00 1265618.00 1910260.00 2126904.00 1493207.00 E 1066938.00 1018492.00 1233062.00 1212505.00 1541824.00 1538328.00 1672271.00 1064728.00 1664575.00 1988020.00 2059646.00 1713490.00 F 760066.00 1055885.00 925075.00 845773.00 844677.00 1142726.00 1154667.00 1249520.00 927607.00 1576475.00 2064476.00 1528446.00 G 1142801.00 1421992.00 1227318.00 1452904.00 1319185.00 1453396.00 1318240.00 1474501.00 1478431.00 1964587.00 1949768.00 1615089.00 H 1217474.00 1248839.00 1221196.00 1502768.00 924943.00 1746322.00 1458289.00 1676866.00 1631411.00 1805675.00 1804494.00 1590364.00 Load time: 08/31/2012 03:31:32 PM Read time: 08/31/2012 03:31:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2880 Comment: adapters 5 uM bottom Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 175890368 cps Min counts: 773935 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26686318.00 41064764.00 63904024.00 74546584.00 98608064.00 46291032.00 56232216.00 73884736.00 41553344.00 87011808.00 78428784.00 87993184.00 B 29305960.00 45930872.00 58134668.00 79394256.00 123208664.00 51003360.00 39813740.00 96835112.00 34339584.00 135355424.00 54557420.00 82795960.00 C 27100374.00 43015280.00 78055440.00 106752632.00 100717744.00 50760500.00 43914956.00 103948104.00 37312648.00 105812704.00 66528712.00 78159480.00 D 31258492.00 47911892.00 141606336.00 133485432.00 103807288.00 44694032.00 51769280.00 124502952.00 31647708.00 132206224.00 76061688.00 88665584.00 E 30133910.00 37906036.00 130674920.00 95241848.00 144923536.00 49515376.00 35395432.00 77257544.00 34050708.00 103499104.00 85278360.00 94851976.00 F 19264436.00 27378008.00 68889160.00 43837100.00 71127216.00 27200478.00 16087085.00 82365080.00 10331879.00 55934720.00 57301368.00 57003408.00 G 32182534.00 52243148.00 112221448.00 105225728.00 144012080.00 44504100.00 31975244.00 127248032.00 25156762.00 175890368.00 94638560.00 80054536.00 H 38729296.00 58507792.00 114781200.00 89445392.00 102787072.00 44444556.00 39933712.00 150410096.00 37045280.00 103871368.00 122254992.00 98988944.00 Load time: 08/31/2012 03:33:33 PM Read time: 08/31/2012 03:33:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2881 Comment: adapters 5 uM 1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 0 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 188296240 cps Min counts: 773935 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29612528.00 43874876.00 67940904.00 77858344.00 102100928.00 47995096.00 59550428.00 77355616.00 43864928.00 90529328.00 82258272.00 92575984.00 B 32396594.00 48757044.00 62062780.00 83184848.00 128682504.00 54065012.00 42188952.00 101275232.00 36327480.00 137816336.00 57560056.00 86481904.00 C 29779222.00 46227992.00 83189320.00 114847640.00 104124320.00 53718272.00 46135404.00 109144848.00 39063172.00 111558224.00 70353912.00 81798360.00 D 34670792.00 51830996.00 151966544.00 143803360.00 107900120.00 47108156.00 55121632.00 137305328.00 33576220.00 137666784.00 79794536.00 90688448.00 E 33256354.00 40666012.00 138616096.00 100956856.00 152307488.00 52442808.00 37425056.00 81876240.00 36061896.00 109340688.00 91175632.00 100948952.00 F 20989098.00 29944332.00 74190704.00 46126604.00 75327296.00 29352640.00 17097458.00 86310232.00 10982213.00 58946732.00 58600824.00 59696496.00 G 35656508.00 56326756.00 120204272.00 110595344.00 152839856.00 47863600.00 33876180.00 133434800.00 26073966.00 188296240.00 98493864.00 83878144.00 H 42719204.00 63486748.00 122944440.00 94810144.00 106573296.00 47631180.00 41948392.00 157675664.00 39049180.00 108085472.00 127674168.00 106533680.00