Load time: 08/29/2012 03:47:34 PM Read time: 08/29/2012 03:47:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2856 Comment: standards+single samples Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 103589712 cps Min counts: 23564 counts Well list: r0(a1:h2,a3:d3,e3:h3) = cps(f2(a1:h2,a3:d3,e3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37493692.00 29811400.00 58819592.00 B 21546704.00 17325380.00 43827316.00 C 11902307.00 8455205.00 103589712.00 D 8968524.00 5242744.00 85995464.00 E 4599787.00 2997532.00 47128.00 F 3123794.00 2768118.00 47921.00 G 2636294.00 1574733.00 48280.00 H 1419768.00 1460965.00 48242.00 Well list: r1(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3) - avg(f2(e3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37445799.25 29763507.25 58771699.25 B 21498811.25 17277487.25 43779423.25 C 11854414.25 8407312.25 103541819.25 D 8920631.25 5194851.25 85947571.25 E 4551894.25 2949639.25 F 3075901.25 2720225.25 G 2588401.25 1526840.25 H 1371875.25 1413072.25 Load time: 08/29/2012 03:48:24 PM Read time: 08/29/2012 03:48:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2857 Comment: standards+single samples 2read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 103940408 cps Min counts: 22903 counts Well list: r0(a1:h2,a3:d3,e3:h3) = cps(f2(a1:h2,a3:d3,e3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37683984.00 29442252.00 60060176.00 B 21351432.00 18192960.00 44916820.00 C 11686676.00 8465974.00 103940408.00 D 9175055.00 5115187.00 89141216.00 E 4503601.00 3028125.00 45806.00 F 3313346.00 2495327.00 46977.00 G 2735851.00 1577910.00 48677.00 H 1488562.00 1593479.00 48186.00 Well list: r1(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3) - avg(f2(e3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37636572.50 29394840.50 60012764.50 B 21304020.50 18145548.50 44869408.50 C 11639264.50 8418562.50 103892996.50 D 9127643.50 5067775.50 89093804.50 E 4456189.50 2980713.50 F 3265934.50 2447915.50 G 2688439.50 1530498.50 H 1441150.50 1546067.50 Load time: 08/29/2012 03:50:06 PM Read time: 08/29/2012 03:50:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2858 Comment: plate1 diluted 25ngul Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15873886 cps Min counts: 24858 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11143871.00 49716.00 9084380.00 12106333.00 8797248.00 14108774.00 9904997.00 7262938.00 7498099.00 10082019.00 6530865.00 7062107.00 B 5256261.00 9904351.00 6246654.00 7784395.00 8975371.00 7009578.00 7416215.00 6557301.00 6631983.00 6921116.00 6900696.00 7392944.00 C 5336996.00 7814169.00 7708674.00 7409994.00 12493176.00 9536655.00 8750900.00 7650394.00 9326195.00 10208190.00 8076768.00 8672302.00 D 5985647.00 14621778.00 9149059.00 7795823.00 11811330.00 6523448.00 8580939.00 6946475.00 7676665.00 7613303.00 8595399.00 9701254.00 E 3122594.00 9610228.00 6521755.00 11289338.00 12697678.00 7320920.00 7269830.00 6985586.00 8461253.00 7800798.00 9527718.00 8586833.00 F 7594769.00 7453387.00 5836814.00 5708042.00 11872774.00 7624768.00 8867900.00 9580905.00 8168082.00 9268046.00 7082587.00 11928751.00 G 5097413.00 8325123.00 13018916.00 8998953.00 13500117.00 7112237.00 8417517.00 8375773.00 9346113.00 10657378.00 7253657.00 8938828.00 H 4327251.00 7756076.00 7448494.00 7442740.00 9886266.00 6689421.00 9429309.00 9955302.00 8883968.00 8439140.00 9212132.00 15873886.00 Load time: 08/29/2012 03:51:50 PM Read time: 08/29/2012 03:51:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2859 Comment: plate1 diluted 25ngul read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15323425 cps Min counts: 24829 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11333276.00 49659.00 8910056.00 11976169.00 8715128.00 13872300.00 9700901.00 7073108.00 7144405.00 9922452.00 6438206.00 7070562.00 B 5253338.00 9783247.00 6020150.00 7753861.00 8875489.00 6888059.00 7123993.00 6276557.00 6605863.00 6848829.00 6733961.00 7320691.00 C 5215644.00 7830880.00 7733439.00 7336686.00 12334520.00 9520742.00 8698040.00 7190364.00 9074141.00 10062625.00 7877226.00 8455786.00 D 5973816.00 14561841.00 9119534.00 7762824.00 11864563.00 6391868.00 8326494.00 6457071.00 7617651.00 7529770.00 8198914.00 9295661.00 E 3111329.00 9613535.00 6605018.00 11352199.00 12474605.00 7209573.00 6911854.00 6558351.00 8067059.00 7781217.00 9153357.00 8137302.00 F 7513296.00 7315237.00 5802682.00 5668190.00 11829842.00 7568603.00 8384033.00 8932564.00 7818235.00 9221421.00 6990617.00 11452111.00 G 5106777.00 8223873.00 12968334.00 8996745.00 13369874.00 7035125.00 7856445.00 7532152.00 8570094.00 10692980.00 7119037.00 8806329.00 H 4171681.00 7700213.00 7481359.00 7429390.00 9641605.00 6511655.00 8681216.00 9109290.00 8841743.00 8465475.00 8303475.00 15323425.00 Load time: 08/29/2012 03:51:50 PM Read time: 08/29/2012 03:51:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2859 Comment: plate1 diluted 25ngul read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15323425 cps Min counts: 24829 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11333276.00 49659.00 8910056.00 11976169.00 8715128.00 13872300.00 9700901.00 7073108.00 7144405.00 9922452.00 6438206.00 7070562.00 B 5253338.00 9783247.00 6020150.00 7753861.00 8875489.00 6888059.00 7123993.00 6276557.00 6605863.00 6848829.00 6733961.00 7320691.00 C 5215644.00 7830880.00 7733439.00 7336686.00 12334520.00 9520742.00 8698040.00 7190364.00 9074141.00 10062625.00 7877226.00 8455786.00 D 5973816.00 14561841.00 9119534.00 7762824.00 11864563.00 6391868.00 8326494.00 6457071.00 7617651.00 7529770.00 8198914.00 9295661.00 E 3111329.00 9613535.00 6605018.00 11352199.00 12474605.00 7209573.00 6911854.00 6558351.00 8067059.00 7781217.00 9153357.00 8137302.00 F 7513296.00 7315237.00 5802682.00 5668190.00 11829842.00 7568603.00 8384033.00 8932564.00 7818235.00 9221421.00 6990617.00 11452111.00 G 5106777.00 8223873.00 12968334.00 8996745.00 13369874.00 7035125.00 7856445.00 7532152.00 8570094.00 10692980.00 7119037.00 8806329.00 H 4171681.00 7700213.00 7481359.00 7429390.00 9641605.00 6511655.00 8681216.00 9109290.00 8841743.00 8465475.00 8303475.00 15323425.00