Load time: 08/28/2012 02:40:40 PM Read time: 08/28/2012 02:40:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2847 Comment: standards plus sample1 1:10 and 1:100 escond read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10674868 cps Min counts: 23186 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7729923.00 8149960.00 7062989.00 7436872.00 7505100.00 7464429.00 6762801.00 9086555.00 3924023.00 7789071.00 6836999.00 46373.00 B 7504154.00 7702051.00 7234599.00 7573983.00 7698319.00 7539370.00 7638227.00 7497252.00 7016239.00 7853874.00 10674868.00 2062420.00 C 6886822.00 7469654.00 7483750.00 8516680.00 7308866.00 6294057.00 7766258.00 7880439.00 8422265.00 7501396.00 8259487.00 8341291.00 D 6560106.00 7910985.00 8088099.00 8942001.00 6215449.00 6612848.00 7265865.00 8707052.00 8634526.00 7299130.00 7749199.00 2686423.00 E 8231622.00 8043176.00 5448524.00 7435871.00 7356447.00 7378736.00 8906839.00 7647747.00 7955103.00 7917527.00 6722597.00 177845.00 F 7153424.00 8348933.00 7341781.00 8356589.00 7397919.00 7006423.00 6984583.00 9058756.00 8170874.00 6924716.00 7178938.00 8798411.00 G 7392681.00 8819624.00 7081172.00 8364638.00 8769572.00 7182223.00 7290271.00 7580120.00 7694698.00 9008571.00 7271792.00 7433749.00 H 7291041.00 7749696.00 9426428.00 8724538.00 7354642.00 6308231.00 9344899.00 7788867.00 7776796.00 8144607.00 8196157.00 7522780.00 Load time: 08/28/2012 02:43:10 PM Read time: 08/28/2012 02:43:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2848 Comment: plate2 15ng\ul Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10722577 cps Min counts: 22289 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7693520.00 8178354.00 7067847.00 7544612.00 7533145.00 7540839.00 6763836.00 9002314.00 3896044.00 7769970.00 6850164.00 44579.00 B 7456064.00 7686297.00 7261255.00 7819149.00 7620676.00 7540883.00 7545722.00 7538848.00 6954799.00 7846832.00 10722577.00 2065406.00 C 6829642.00 7458515.00 7501585.00 8531311.00 7109803.00 6200740.00 7709180.00 7856385.00 8503380.00 7616017.00 8345583.00 8440394.00 D 6488548.00 7995697.00 8034622.00 9001299.00 6241854.00 6573152.00 7329186.00 8605532.00 8758801.00 7246912.00 7817370.00 2691436.00 E 8209307.00 8093743.00 5408993.00 7399291.00 7395160.00 7283553.00 8887307.00 7589399.00 7931990.00 7919593.00 6778062.00 176749.00 F 7136750.00 8308026.00 7380059.00 8391273.00 7384856.00 7096376.00 7003746.00 9073778.00 8240494.00 6855020.00 7208836.00 8786722.00 G 7557028.00 8852489.00 7261644.00 8523012.00 8751786.00 7140676.00 7290553.00 7538664.00 7743468.00 8956369.00 7258006.00 7458716.00 H 7306554.00 7733391.00 9440230.00 8950204.00 7375338.00 6222092.00 9361326.00 7771195.00 7793622.00 8261796.00 8241029.00 7599029.00 Load time: 08/28/2012 02:48:58 PM Read time: 08/28/2012 02:48:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2849 Comment: plate2 15ng\ul sample A12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33356318 cps Min counts: 84285 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7409565.00 7969705.00 7026045.00 6914498.00 7466267.00 6941508.00 6837373.00 8939707.00 3975524.00 7762050.00 6731265.00 33356318.00 B 7242414.00 7577381.00 7115760.00 7311649.00 6767255.00 6817961.00 7118568.00 6637401.00 6936959.00 7854520.00 10640383.00 1980231.00 C 6600860.00 7201201.00 5689247.00 7251938.00 6899312.00 5804024.00 7613439.00 7681115.00 7694958.00 7420809.00 8242425.00 8078987.00 D 6133826.00 7725978.00 7681154.00 8631934.00 6135684.00 5578618.00 6628297.00 8460042.00 6941365.00 7169478.00 7465308.00 2855110.00 E 7909255.00 7767326.00 5262311.00 7153854.00 6648094.00 7094411.00 7577551.00 6862638.00 7827787.00 7770079.00 6624660.00 168570.00 F 6831511.00 8026679.00 7192165.00 8182810.00 7135473.00 6735924.00 6282206.00 8849069.00 8024349.00 6804152.00 7133545.00 8294871.00 G 6641919.00 8640900.00 6913553.00 6882552.00 8523210.00 6777177.00 6250260.00 7371937.00 7511381.00 8738452.00 7110548.00 6416581.00 H 6887128.00 7418773.00 8941671.00 8272628.00 7028618.00 5763526.00 8975487.00 6931148.00 7623995.00 7906733.00 7976886.00 7256705.00 Load time: 08/28/2012 02:48:58 PM Read time: 08/28/2012 02:48:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2849 Comment: plate2 15ng\ul sample A12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33356318 cps Min counts: 84285 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7409565.00 7969705.00 7026045.00 6914498.00 7466267.00 6941508.00 6837373.00 8939707.00 3975524.00 7762050.00 6731265.00 33356318.00 B 7242414.00 7577381.00 7115760.00 7311649.00 6767255.00 6817961.00 7118568.00 6637401.00 6936959.00 7854520.00 10640383.00 1980231.00 C 6600860.00 7201201.00 5689247.00 7251938.00 6899312.00 5804024.00 7613439.00 7681115.00 7694958.00 7420809.00 8242425.00 8078987.00 D 6133826.00 7725978.00 7681154.00 8631934.00 6135684.00 5578618.00 6628297.00 8460042.00 6941365.00 7169478.00 7465308.00 2855110.00 E 7909255.00 7767326.00 5262311.00 7153854.00 6648094.00 7094411.00 7577551.00 6862638.00 7827787.00 7770079.00 6624660.00 168570.00 F 6831511.00 8026679.00 7192165.00 8182810.00 7135473.00 6735924.00 6282206.00 8849069.00 8024349.00 6804152.00 7133545.00 8294871.00 G 6641919.00 8640900.00 6913553.00 6882552.00 8523210.00 6777177.00 6250260.00 7371937.00 7511381.00 8738452.00 7110548.00 6416581.00 H 6887128.00 7418773.00 8941671.00 8272628.00 7028618.00 5763526.00 8975487.00 6931148.00 7623995.00 7906733.00 7976886.00 7256705.00