Load time: 08/28/2012 10:10:02 AM Read time: 08/28/2012 10:10:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2835 Comment: Standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35810308 cps Min counts: 19575 counts Well list: r0(a1:h2,a3:h3) = cps(f2(a1:h2,a3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35810308.00 34251188.00 48220.00 B 28238706.00 19871282.00 44441.00 C 29318162.00 14583701.00 42589.00 D 8875932.00 10500358.00 40511.00 E 4439868.00 3941639.00 39150.00 F 2561110.00 2943054.00 40813.00 G 1552105.00 1779647.00 46690.00 H 998459.00 1055900.00 52396.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35765956.75 34206836.75 B 28194354.75 19826930.75 C 29273810.75 14539349.75 D 8831580.75 10456006.75 E 4395516.75 3897287.75 F 2516758.75 2898702.75 G 1507753.75 1735295.75 H 954107.75 1011548.75 Load time: 08/28/2012 10:12:19 AM Read time: 08/28/2012 10:12:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2836 Comment: plate1 #1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 144073184 cps Min counts: 773946 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 144073184.00 2095228.00 20519622.00 36467016.00 17271778.00 35291204.00 20837732.00 8542194.00 2933478.00 40262964.00 9986203.00 14290508.00 B 5045660.00 17736428.00 14444453.00 15689933.00 14390763.00 7256129.00 8180615.00 10601153.00 16324650.00 11370835.00 17443414.00 13654165.00 C 7429344.00 24881828.00 24897438.00 6091972.00 21348372.00 8975127.00 3565130.00 6238879.00 19320362.00 17504562.00 17553354.00 11290795.00 D 14567861.00 23498790.00 35861748.00 13295837.00 23965726.00 7396774.00 11887890.00 6507844.00 11273328.00 13990689.00 18386896.00 16672915.00 E 2637114.00 33983044.00 23678090.00 33480998.00 24498574.00 6244709.00 3917071.00 11794031.00 17981950.00 10369687.00 27289796.00 23619268.00 F 12648462.00 12697268.00 19852882.00 10740966.00 19473886.00 4986789.00 2847042.00 15834451.00 9393412.00 19016636.00 12693687.00 26214066.00 G 2946256.00 13317342.00 53273132.00 6830438.00 47621628.00 7353602.00 5587675.00 11885259.00 13397777.00 5478834.00 16209089.00 11934801.00 H 4669049.00 10169205.00 19281328.00 4148938.00 23921354.00 7184407.00 10607997.00 19013742.00 12650179.00 12096264.00 16002206.00 30585340.00 Load time: 08/28/2012 10:14:01 AM Read time: 08/28/2012 10:14:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2837 Comment: plate1 #1 second read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 143584048 cps Min counts: 773946 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 143584048.00 2097985.00 20283484.00 36367288.00 17652364.00 35303600.00 20843512.00 8511990.00 2935371.00 41604228.00 10016394.00 14269744.00 B 5037443.00 17778310.00 14437824.00 15516765.00 14682218.00 7307644.00 8318581.00 10779413.00 16700691.00 11513945.00 17736672.00 14026390.00 C 7419196.00 24877110.00 24658274.00 6065841.00 21502044.00 8899848.00 3514588.00 6289743.00 19458024.00 17557736.00 17801538.00 11443973.00 D 14442566.00 23191638.00 35290564.00 13166994.00 23637376.00 7409436.00 11823996.00 6551098.00 11312976.00 14012244.00 18658354.00 16724330.00 E 2656002.00 34168628.00 23428934.00 33773648.00 24279310.00 6301281.00 3888447.00 11711223.00 17991690.00 10394086.00 27204136.00 25100594.00 F 12540902.00 12638423.00 19822170.00 10750067.00 19215312.00 4905914.00 2825912.00 15898072.00 9520050.00 19062916.00 13092422.00 26247940.00 G 2945405.00 13265369.00 53061664.00 6824975.00 47702936.00 7353748.00 5581707.00 12013833.00 13407387.00 5448039.00 16421529.00 12262582.00 H 4661136.00 10142234.00 19265730.00 4139385.00 23785936.00 7171106.00 10502823.00 19039372.00 12714645.00 12120606.00 16429479.00 30328848.00 Load time: 08/28/2012 10:16:28 AM Read time: 08/28/2012 10:16:28 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2838 Comment: plate1 #2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 141953888 cps Min counts: 773946 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 141953888.00 2034020.00 19080586.00 38518420.00 21936770.00 40183940.00 25294030.00 7761790.00 3149802.00 46733092.00 12072846.00 13838246.00 B 4559805.00 14577766.00 14706295.00 16876438.00 16502380.00 7786724.00 7896022.00 9634831.00 17874884.00 13381262.00 23468222.00 14915602.00 C 7206758.00 27603492.00 30429612.00 5852817.00 30641648.00 8941356.00 3479635.00 5971023.00 21901882.00 17797158.00 24312184.00 11709134.00 D 15735902.00 20236546.00 37157352.00 13627406.00 22103732.00 7544318.00 12279391.00 6213834.00 11235247.00 12577547.00 27263360.00 21505212.00 E 2362976.00 35408748.00 24567008.00 34071008.00 25589414.00 6431611.00 3650928.00 12475125.00 24645314.00 9936904.00 35088960.00 30909528.00 F 13064884.00 11443990.00 21263324.00 10353595.00 22406610.00 5275329.00 2751689.00 17002906.00 9713796.00 23121040.00 13925715.00 29216010.00 G 2626898.00 13216146.00 55215652.00 6263357.00 49045432.00 7327432.00 6008432.00 14002055.00 13413358.00 5241301.00 27012718.00 12757987.00 H 4429891.00 9978992.00 21620438.00 4182083.00 27597194.00 6960628.00 11138119.00 20739456.00 13919280.00 12904618.00 23720542.00 30934606.00 Load time: 08/28/2012 10:18:23 AM Read time: 08/28/2012 10:18:23 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2839 Comment: plate1 #2 second read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 144320352 cps Min counts: 773946 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 144320352.00 2001906.00 19163170.00 38651340.00 21790636.00 40376392.00 25448728.00 7681922.00 3148274.00 46704044.00 12456531.00 13931231.00 B 4592956.00 14818531.00 15023746.00 17015202.00 16706459.00 7842486.00 8089220.00 9731923.00 18023238.00 13537163.00 23617106.00 15315822.00 C 7240537.00 28105500.00 30915082.00 5904281.00 31067974.00 8928317.00 3417704.00 6066136.00 21995558.00 17980154.00 24570206.00 11819734.00 D 15650242.00 20066652.00 37718904.00 13622706.00 22148580.00 7576245.00 12196090.00 6238879.00 11215888.00 12724029.00 27362540.00 21705020.00 E 2381417.00 35749932.00 24747528.00 34263928.00 25659578.00 6458932.00 3672489.00 12587898.00 24708498.00 9954120.00 35496284.00 31797452.00 F 13049266.00 11591860.00 21215180.00 10408176.00 22327812.00 5232131.00 2720264.00 17070936.00 9785992.00 23121454.00 14167427.00 29681906.00 G 2656801.00 13215806.00 55886280.00 6183945.00 49615468.00 7407734.00 6047338.00 14138131.00 13622274.00 5234937.00 27243396.00 12828769.00 H 4449680.00 10001676.00 21635546.00 4113616.00 27746194.00 7043140.00 11223793.00 20786080.00 14213689.00 13333334.00 24631200.00 30699014.00 Load time: 08/28/2012 10:20:53 AM Read time: 08/28/2012 10:20:53 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2840 Comment: plate2 #1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 201119152 cps Min counts: 36666 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 151843600.00 172590320.00 125937152.00 127120336.00 113605192.00 74299360.00 129691456.00 17192664.00 36631848.00 84503080.00 46749184.00 73332.00 B 115747664.00 106258816.00 166622912.00 100609248.00 93312936.00 64002744.00 173476208.00 79935840.00 112351816.00 46866668.00 14196402.00 1391668.00 C 88205096.00 136387888.00 156990352.00 97793400.00 128678008.00 123090960.00 120917840.00 34293080.00 83104808.00 100150984.00 78199344.00 91148000.00 D 138328272.00 181247904.00 105107256.00 28478216.00 194904624.00 137815936.00 161467648.00 28291872.00 79075008.00 91080424.00 59896508.00 1940861.00 E 165734464.00 189025696.00 143720032.00 158255120.00 151602640.00 43611968.00 98797096.00 97077040.00 91708552.00 34323952.00 48570812.00 178502.00 F 145837808.00 131522928.00 132513792.00 173111472.00 73641888.00 160549872.00 143866960.00 132809400.00 176333808.00 61697088.00 81813416.00 40092352.00 G 161751120.00 186939152.00 117914976.00 67161896.00 201119152.00 119973152.00 143198832.00 125183464.00 165412112.00 27204994.00 81980664.00 41460028.00 H 156457184.00 161832304.00 62447792.00 191718096.00 136549120.00 94000872.00 36704816.00 62068672.00 55196752.00 58720856.00 89366168.00 37851176.00 Load time: 08/28/2012 10:22:24 AM Read time: 08/28/2012 10:22:24 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2841 Comment: plate2 #1 second read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 200582288 cps Min counts: 36524 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 152990208.00 171580192.00 123347952.00 127235264.00 111746728.00 74120048.00 127097376.00 16985218.00 36554336.00 84240016.00 46973332.00 73048.00 B 114333560.00 105104400.00 165826768.00 99752120.00 92599520.00 64231680.00 169922656.00 80439304.00 112825160.00 46780264.00 14089025.00 1351780.00 C 88031528.00 135803952.00 156761408.00 99447048.00 127337856.00 122582112.00 122278064.00 34150684.00 82943608.00 99708416.00 78371968.00 90196224.00 D 140151872.00 176934464.00 103668392.00 28064120.00 192361376.00 139558000.00 162015216.00 28318060.00 79420688.00 91052552.00 59862688.00 1922674.00 E 164312928.00 182806224.00 146408896.00 160499936.00 151182144.00 43215036.00 98887976.00 97170896.00 90994752.00 34353960.00 48830372.00 175177.00 F 143307536.00 131496128.00 134768192.00 176991136.00 73767528.00 161225456.00 143077056.00 132054792.00 174942752.00 62615024.00 81343064.00 40107308.00 G 165713168.00 187960640.00 116141952.00 67884704.00 200582288.00 119961984.00 144204720.00 126608760.00 164526000.00 27158696.00 81680448.00 41225932.00 H 154888480.00 164260624.00 61928124.00 194000816.00 138931680.00 94203392.00 36817440.00 62654564.00 55493580.00 58564004.00 89304296.00 37812336.00 Load time: 08/28/2012 10:24:29 AM Read time: 08/28/2012 10:24:29 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2842 Comment: plate2 #2 first read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 184299376 cps Min counts: 35428 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 159019264.00 168487360.00 123452232.00 143077056.00 119404904.00 74270840.00 131818632.00 15093241.00 33455524.00 87821752.00 44598112.00 70856.00 B 135442704.00 111830696.00 160606512.00 101606480.00 96479232.00 64172560.00 170657056.00 87617944.00 102417264.00 47379080.00 14051474.00 1435070.00 C 85862432.00 138412400.00 156495152.00 103321016.00 123932608.00 124177192.00 132434416.00 31653874.00 85252384.00 106225280.00 94862696.00 98365128.00 D 141491920.00 175661424.00 75318160.00 20743068.00 167488304.00 136551520.00 150120592.00 26802886.00 93359072.00 89904944.00 65233788.00 1869878.00 E 174465584.00 164319888.00 103673944.00 155417232.00 151176240.00 29999678.00 121420560.00 89771464.00 93092936.00 25535550.00 42164988.00 180845.00 F 154542064.00 113966536.00 109837328.00 164529488.00 71494920.00 144874160.00 137231904.00 129320896.00 168388384.00 54710188.00 62148420.00 56732228.00 G 170747392.00 172571072.00 107661576.00 70674896.00 184299376.00 108359472.00 160873568.00 131728888.00 163087232.00 24319136.00 84576952.00 43316380.00 H 162361888.00 164211840.00 59356756.00 167909824.00 130632736.00 88272496.00 36959324.00 48170560.00 56298912.00 63249032.00 81083152.00 43283676.00 Load time: 08/28/2012 10:25:58 AM Read time: 08/28/2012 10:25:58 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2843 Comment: plate2 #2 second read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 183095984 cps Min counts: 35041 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 160866896.00 168274896.00 123493600.00 143661328.00 120768776.00 73957144.00 133069704.00 15006559.00 33397058.00 87661600.00 44619972.00 70082.00 B 135345600.00 113545192.00 163538656.00 101238320.00 96017224.00 64339540.00 170517920.00 86492864.00 102246784.00 47628372.00 14230049.00 1424281.00 C 85353936.00 138849424.00 156441392.00 105177240.00 127509880.00 126354520.00 133854504.00 31761302.00 86088776.00 107744008.00 93788984.00 100337952.00 D 144787440.00 177886096.00 75517312.00 20818002.00 165642224.00 136092896.00 149355776.00 26716746.00 92742648.00 89405392.00 65582596.00 1890841.00 E 173301424.00 165147392.00 102712208.00 157784096.00 153250704.00 29820974.00 119138376.00 90179408.00 94812728.00 25392454.00 42656500.00 179768.00 F 153811120.00 113059192.00 111869496.00 164323376.00 71874024.00 146678064.00 138204784.00 129587232.00 166852816.00 54880884.00 62600844.00 57428084.00 G 170634464.00 168146944.00 108351888.00 70880088.00 183095984.00 106982952.00 161700432.00 131491656.00 164414160.00 24509128.00 85446272.00 43308140.00 H 163097536.00 159133712.00 59026736.00 165880096.00 133331048.00 89945696.00 37099464.00 48109776.00 56019768.00 63067092.00 82018904.00 42902656.00 Load time: 08/28/2012 10:28:30 AM Read time: 08/28/2012 10:28:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2844 Comment: standards plus sample1 1:10 and 1:100 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 34526532 cps Min counts: 17638 counts Well list: r0(a1:h2,a3:d3,e3:h3) = cps(f2(a1:h2,a3:d3,e3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34526532.00 32134640.00 18674068.00 B 26650598.00 19506722.00 18718010.00 C 29377588.00 14237587.00 2099581.00 D 9259198.00 10372828.00 2072041.00 E 4403389.00 3857736.00 35277.00 F 2532690.00 2782591.00 35711.00 G 1557568.00 1712022.00 41531.00 H 1520061.00 973498.00 46142.00 Well list: r1(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3) - avg(f2(e3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34486866.75 32094974.75 18634402.75 B 26610932.75 19467056.75 18678344.75 C 29337922.75 14197921.75 2059915.75 D 9219532.75 10333162.75 2032375.75 E 4363723.75 3818070.75 F 2493024.75 2742925.75 G 1517902.75 1672356.75 H 1480395.75 933832.75 Load time: 08/28/2012 10:29:36 AM Read time: 08/28/2012 10:29:36 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2845 Comment: standards plus sample1 1:10 and 1:100 escond read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33992440 cps Min counts: 17449 counts Well list: r0(a1:h2,a3:d3,e3:h3) = cps(f2(a1:h2,a3:d3,e3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33992440.00 32057306.00 18256044.00 B 26752210.00 19292238.00 18562988.00 C 29923474.00 14533829.00 2092908.00 D 8741496.00 10585464.00 2081788.00 E 4425362.00 3968656.00 34899.00 F 2592454.00 2838444.00 34918.00 G 1499446.00 1742138.00 41456.00 H 1119143.00 994094.00 45329.00 Well list: r1(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3) - avg(f2(e3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33953289.50 32018155.50 18216893.50 B 26713059.50 19253087.50 18523837.50 C 29884323.50 14494678.50 2053757.50 D 8702345.50 10546313.50 2042637.50 E 4386211.50 3929505.50 F 2553303.50 2799293.50 G 1460295.50 1702987.50 H 1079992.50 954943.50 Load time: 08/28/2012 10:29:36 AM Read time: 08/28/2012 10:29:36 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2845 Comment: standards plus sample1 1:10 and 1:100 escond read Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33992440 cps Min counts: 17449 counts Well list: r0(a1:h2,a3:d3,e3:h3) = cps(f2(a1:h2,a3:d3,e3:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33992440.00 32057306.00 18256044.00 B 26752210.00 19292238.00 18562988.00 C 29923474.00 14533829.00 2092908.00 D 8741496.00 10585464.00 2081788.00 E 4425362.00 3968656.00 34899.00 F 2592454.00 2838444.00 34918.00 G 1499446.00 1742138.00 41456.00 H 1119143.00 994094.00 45329.00 Well list: r1(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3) - avg(f2(e3:h3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33953289.50 32018155.50 18216893.50 B 26713059.50 19253087.50 18523837.50 C 29884323.50 14494678.50 2053757.50 D 8702345.50 10546313.50 2042637.50 E 4386211.50 3929505.50 F 2553303.50 2799293.50 G 1460295.50 1702987.50 H 1079992.50 954943.50