Load time: 07/26/2011 03:23:20 PM Read time: 07/26/2011 03:23:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1738 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 62560580 cps Min counts: 61866 counts Well list: r0(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62560580.00 9297364.00 123732.00 B 43934892.00 43957844.00 126515.00 C 24063384.00 24408368.00 131543.00 D 14585221.00 14480494.00 128311.00 E 8956160.00 8844057.00 F 5385939.00 5519189.00 G 3338343.00 3439088.00 H 2122392.00 2142325.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:d3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62433054.75 9169838.75 B 43807366.75 43830318.75 C 23935858.75 24280842.75 D 14457695.75 14352968.75 E 8828634.75 8716531.75 F 5258413.75 5391663.75 G 3210817.75 3311562.75 H 1994866.75 2014799.75 Load time: 07/26/2011 03:23:20 PM Read time: 07/26/2011 03:23:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1738 Comment: noug 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: -1.#INF cps Min counts: 1.#INF counts Well list: r0(a1:h2,a3:d3) = cps(f2(a1:h2,a3:d3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:d3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H Load time: 07/26/2011 03:25:48 PM Read time: 07/26/2011 03:25:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1740 Comment: noug 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 59297472 cps Min counts: 1522363 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7221269.00 4373455.00 7781675.00 3044726.00 26822014.00 18413604.00 18593200.00 27765622.00 26342598.00 20431630.00 29843198.00 B 47195880.00 44593752.00 12132381.00 3054962.00 14088307.00 14453511.00 21164860.00 12439589.00 52363172.00 18903590.00 30485314.00 C 24882536.00 40083732.00 46528468.00 59297472.00 33699784.00 30283796.00 18636096.00 31380936.00 13868415.00 23089220.00 26062384.00 D 28125568.00 33149342.00 37624988.00 50058356.00 26725674.00 36390004.00 17882564.00 12863867.00 29336750.00 37618988.00 32186026.00 E 26314194.00 27961750.00 44037108.00 20520160.00 13170147.00 29186240.00 34454568.00 20648304.00 23074344.00 43256728.00 12841809.00 F 30711290.00 39265924.00 30581740.00 34535712.00 25949548.00 14304305.00 12115947.00 21487092.00 13898572.00 21995892.00 19911562.00 G 17445508.00 16681391.00 13547839.00 18583572.00 11364657.00 12636898.00 14643454.00 14716439.00 17754336.00 17948602.00 14804330.00 H 14177361.00 13142315.00 17385480.00 17706368.00 10950452.00 10292060.00 7346438.00 13441283.00 19256660.00 17280702.00 14168376.00 Load time: 07/26/2011 03:27:55 PM Read time: 07/26/2011 03:27:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1741 Comment: noug 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 60225268 cps Min counts: 1515583 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7373931.00 4437476.00 7925010.00 3031167.00 27104104.00 18576250.00 19138676.00 27832482.00 26689444.00 20538966.00 30403714.00 28180264.00 B 47347716.00 44872316.00 12151904.00 3071124.00 14234945.00 14718937.00 21752408.00 12563534.00 52858668.00 19033082.00 30915568.00 34025716.00 C 24941376.00 40308484.00 46915448.00 60225268.00 33571968.00 30344964.00 18964138.00 31534738.00 13911340.00 23697334.00 26238630.00 20968854.00 D 28268830.00 33371076.00 38028576.00 49978020.00 26527700.00 36455076.00 17927352.00 12940568.00 29303090.00 37907736.00 32711738.00 44935748.00 E 26650782.00 28356710.00 44571428.00 20553854.00 13238647.00 29356748.00 34734704.00 20700638.00 23131216.00 43681816.00 12856448.00 25747108.00 F 30725734.00 39693904.00 30953922.00 34951180.00 25868914.00 14360308.00 12174297.00 21578112.00 13919338.00 22183194.00 19986158.00 40493636.00 G 17610428.00 16813796.00 13737666.00 18780420.00 11464161.00 12716850.00 14801690.00 14784105.00 17759094.00 18099950.00 14929454.00 14405677.00 H 14361822.00 13314832.00 17619776.00 18154566.00 11033746.00 10404754.00 7383138.00 13461237.00 19243570.00 17285232.00 14171035.00 11150097.00 Load time: 07/26/2011 03:29:59 PM Read time: 07/26/2011 03:29:59 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1742 Comment: noug 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 28808680 cps Min counts: 2969301 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28808680.00 10664222.00 16431977.00 21618282.00 9023725.00 18037042.00 19624392.00 15240515.00 10178279.00 11071020.00 16564451.00 13666054.00 B 11468458.00 14839137.00 17310412.00 12936453.00 24854908.00 15083314.00 17294118.00 9104143.00 16199595.00 18444704.00 24016246.00 13239388.00 C 11716671.00 12917099.00 11117687.00 20266522.00 11426670.00 13211330.00 11938595.00 14805672.00 15166025.00 12359150.00 15286504.00 12555588.00 D 11813058.00 12518420.00 9827284.00 18182544.00 12668245.00 16332373.00 14919572.00 14691304.00 17720454.00 10264006.00 17747124.00 15011702.00 E 17096552.00 19205816.00 17005398.00 19133538.00 17114510.00 14698854.00 20556024.00 13529668.00 12847524.00 15343831.00 17098772.00 11979679.00 F 10124341.00 11088340.00 13789562.00 9145451.00 11177097.00 20514416.00 12851187.00 18865694.00 14323346.00 14864288.00 12229786.00 19742862.00 G 13862284.00 11240063.00 10265954.00 14102845.00 12002463.00 17527852.00 14533418.00 20604172.00 17309524.00 21664086.00 10651574.00 12119293.00 H 5938603.00 8144796.00 13445358.00 10044100.00 13202410.00 12686115.00 12974601.00 12635336.00 18004678.00 13331806.00 13414025.00 20266778.00 Load time: 07/26/2011 03:32:25 PM Read time: 07/26/2011 03:32:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1743 Comment: noug 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 45961544 cps Min counts: 65053 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13454254.00 14138861.00 14138029.00 16430297.00 8979322.00 10331542.00 9093971.00 6379926.00 7745736.00 14126712.00 10830529.00 8309456.00 B 13156696.00 12128068.00 9006118.00 12057063.00 8670429.00 13074780.00 15413622.00 16780486.00 17804566.00 21712700.00 17035790.00 14405316.00 C 15994390.00 21632918.00 12204022.00 12754238.00 12647058.00 9813990.00 9176701.00 7022202.00 18735042.00 13079439.00 16768788.00 30924044.00 D 15034240.00 17496000.00 17301146.00 12808482.00 19254714.00 8506124.00 26722234.00 11651009.00 12235120.00 21617036.00 38584896.00 7081015.00 E 12852831.00 26350450.00 38586228.00 13519248.00 11738086.00 12599564.00 21134378.00 25008382.00 12059209.00 27468214.00 9940218.00 10978461.00 F 36609876.00 29297536.00 17869418.00 21852910.00 16679855.00 38111020.00 11083923.00 45961544.00 14084401.00 19180676.00 15854623.00 21801316.00 G 25353802.00 15689017.00 12417293.00 14634791.00 24858956.00 12050022.00 14648570.00 18921416.00 14982220.00 33501784.00 18699542.00 18968220.00 H 26573508.00 22234130.00 16443357.00 20228444.00 10324186.00 16979068.00 152106.00 144114.00 142767.00 139086.00 130107.00 139265.00 Load time: 07/26/2011 03:34:32 PM Read time: 07/26/2011 03:34:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1744 Comment: noug 3 sens Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46035140 cps Min counts: 67552 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13794358.00 14336871.00 14453257.00 16609365.00 9110201.00 10386114.00 9066299.00 6422161.00 7756126.00 14146450.00 10859732.00 8294505.00 B 13501976.00 12519000.00 9124051.00 12128181.00 8679363.00 13115239.00 15318032.00 16701376.00 18045094.00 21823916.00 17179050.00 14435862.00 C 16479955.00 22248786.00 12414701.00 12852908.00 12778606.00 9867587.00 9137568.00 6956083.00 18755054.00 13034810.00 16893064.00 30711736.00 D 15342269.00 17673266.00 17342836.00 12941103.00 19208114.00 8556239.00 26737152.00 11665401.00 12262282.00 21599782.00 38820408.00 7082799.00 E 13107169.00 26796424.00 38740352.00 13507592.00 11759021.00 12682714.00 21160508.00 25065004.00 12109383.00 27780560.00 9982126.00 10976497.00 F 36492748.00 29007234.00 18112760.00 22000666.00 16595824.00 38167044.00 11047093.00 46035140.00 14125279.00 19221940.00 15866730.00 21682560.00 G 26080650.00 16110967.00 12489679.00 14839723.00 24809688.00 12093133.00 14680478.00 18912376.00 14864027.00 33371940.00 18647438.00 18898670.00 H 27033544.00 22171722.00 16745174.00 20534132.00 10367772.00 17019482.00 149609.00 141405.00 143042.00 139692.00 135104.00 136932.00