Load time: 07/29/2011 02:48:06 PM Read time: 07/29/2011 02:48:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1748 Comment: noug F3 standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 78160992 cps Min counts: 17078 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 59253288.00 78160992.00 34157.00 B 42648416.00 57211436.00 44311.00 C 27062138.00 21041642.00 D 17745668.00 13277011.00 E 10663182.00 8806244.00 F 5490086.00 4384371.00 G 2711142.00 2242987.00 H 2787534.00 2461275.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 59214054.00 78121758.00 B 42609182.00 57172202.00 C 27022904.00 21002408.00 D 17706434.00 13237777.00 E 10623948.00 8767010.00 F 5450852.00 4345137.00 G 2671908.00 2203753.00 H 2748300.00 2422041.00 Load time: 07/29/2011 02:53:21 PM Read time: 07/29/2011 02:53:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1749 Comment: noug F3 standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 85159992 cps Min counts: 16974 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85159992.00 78017208.00 33948.00 B 43963496.00 58214464.00 44787.00 C 27060732.00 21121550.00 D 17341432.00 13003324.00 E 10294020.00 8388499.00 F 5317363.00 4212151.00 G 2609104.00 2171733.00 H 2882527.00 2327841.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85120624.50 77977840.50 B 43924128.50 58175096.50 C 27021364.50 21082182.50 D 17302064.50 12963956.50 E 10254652.50 8349131.50 F 5277995.50 4172783.50 G 2569736.50 2132365.50 H 2843159.50 2288473.50 Load time: 07/29/2011 02:54:28 PM Read time: 07/29/2011 02:54:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1750 Comment: noug F3 standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 85358424 cps Min counts: 16621 counts Well list: r0(a1:h2,a3:b3) = cps(f2(a1:h2,a3:b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85358424.00 79156848.00 33243.00 B 44993456.00 58621628.00 43625.00 C 27814460.00 21392844.00 D 17750472.00 13068349.00 E 10363981.00 8430707.00 F 5318980.00 4266250.00 G 2623405.00 2210805.00 H 3038643.00 2354042.00 Well list: r1(a1:h2) = cps(f2(a1:h2) - avg(f2(a3:b3))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85319990.00 79118414.00 B 44955022.00 58583194.00 C 27776026.00 21354410.00 D 17712038.00 13029915.00 E 10325547.00 8392273.00 F 5280546.00 4227816.00 G 2584971.00 2172371.00 H 3000209.00 2315608.00 Load time: 07/29/2011 02:56:08 PM Read time: 07/29/2011 02:56:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1751 Comment: noug F3 samples Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 117019952 cps Min counts: 183047 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 58454312.00 25889682.00 117019952.00 28402680.00 27549222.00 5965801.00 17039848.00 26789914.00 23538410.00 35782332.00 18825254.00 54428380.00 B 48833928.00 21567866.00 13040831.00 15437211.00 21302668.00 47591636.00 10936711.00 20595186.00 32482298.00 80500184.00 9544523.00 8351438.00 C 30853486.00 23355934.00 49617364.00 17511500.00 366095.00 22024122.00 15118790.00 9063841.00 15335155.00 26177252.00 23355374.00 15804820.00 D 8318763.00 10360679.00 6131809.00 8063395.00 19328012.00 25875690.00 12623351.00 13545046.00 29459206.00 19951788.00 22463114.00 16339568.00 E 6908208.00 17543746.00 27917556.00 32453658.00 107414472.00 9271201.00 19172060.00 25626952.00 28859096.00 21929424.00 12504947.00 51890068.00 F 25254548.00 11758387.00 12022528.00 19714668.00 39162676.00 5197163.00 13039850.00 7543821.00 5842490.00 12749455.00 13912165.00 20204828.00 G 24040460.00 28158332.00 18143300.00 48223696.00 30880106.00 12633999.00 11448784.00 19433188.00 9572130.00 28712024.00 23904070.00 29133856.00 H 13787463.00 45321420.00 17402654.00 51961596.00 26575770.00 17555786.00 16253090.00 12820065.00 49877756.00 17550494.00 13603775.00 58958696.00 Load time: 07/29/2011 02:57:49 PM Read time: 07/29/2011 02:57:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1752 Comment: noug F3 samples Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 119390512 cps Min counts: 179904 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 58179740.00 25780112.00 119390512.00 28280594.00 27558368.00 5996388.00 17038730.00 26905936.00 23716908.00 36588692.00 19137642.00 54979068.00 B 48529008.00 22166836.00 13056629.00 15492442.00 21312384.00 47754740.00 11232365.00 20513702.00 32646462.00 80539232.00 9597161.00 8326548.00 C 30556392.00 23172620.00 49579536.00 17568392.00 359808.00 21964856.00 14989549.00 9084937.00 15445094.00 26305654.00 23254176.00 16053764.00 D 8353236.00 10370538.00 6280661.00 7999881.00 19288454.00 26319316.00 12635822.00 13767078.00 29490934.00 20189144.00 22556424.00 16349463.00 E 6985049.00 17470256.00 28297106.00 32398682.00 107500304.00 9276436.00 19214836.00 25613574.00 29150616.00 22068222.00 12489735.00 52041648.00 F 25041236.00 11823328.00 12052814.00 19627688.00 38942412.00 5209835.00 13131711.00 7595390.00 5900087.00 12714160.00 13781522.00 20509494.00 G 23885482.00 28057086.00 18072328.00 48455776.00 31185306.00 12824574.00 11504656.00 19441612.00 9585206.00 28213106.00 23892872.00 29080544.00 H 13866970.00 45126680.00 17309120.00 51866960.00 26551896.00 17468808.00 16179757.00 13025702.00 50440692.00 17799830.00 13841758.00 60713364.00