Load time: 03/26/2013 03:32:38 PM Read time: 03/26/2013 03:32:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3001 Comment: plate_1_repeat Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 51936528 cps Min counts: 10937 counts Well list: r0(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21874.00 51936528.00 44842272.00 3851069.00 2865262.00 12877757.00 22081222.00 28270164.00 B 23912.00 34224276.00 3138099.00 6021923.00 22710438.00 4226691.00 25761786.00 3965915.00 C 24484.00 21084730.00 1407867.00 3690435.00 10468953.00 517463.00 5454193.00 6553218.00 D 24775.00 12934413.00 1509692.00 26407994.00 18836264.00 3438408.00 4638268.00 2448369.00 E 7412229.00 6626634.00 4149861.00 6649505.00 2794246.00 11382247.00 1489128.00 F 3665859.00 23406890.00 12067465.00 6696286.00 27688372.00 9107887.00 12541440.00 G 2444102.00 5095961.00 46464400.00 2972012.00 2061609.00 26449350.00 1028406.00 H 1276900.00 34592664.00 12645732.00 4697696.00 13117808.00 5767999.00 1887891.00 Well list: r1(a2,a3,a4,a5,a6,a7,a8,b2:h8) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51912766.75 44818510.75 3827307.75 2841500.75 12853995.75 22057460.75 28246402.75 B 34200514.75 3114337.75 5998161.75 22686676.75 4202929.75 25738024.75 3942153.75 C 21060968.75 1384105.75 3666673.75 10445191.75 493701.75 5430431.75 6529456.75 D 12910651.75 1485930.75 26384232.75 18812502.75 3414646.75 4614506.75 2424607.75 E 7388467.75 6602872.75 4126099.75 6625743.75 2770484.75 11358485.75 1465366.75 F 3642097.75 23383128.75 12043703.75 6672524.75 27664610.75 9084125.75 12517678.75 G 2420340.75 5072199.75 46440638.75 2948250.75 2037847.75 26425588.75 1004644.75 H 1253138.75 34568902.75 12621970.75 4673934.75 13094046.75 5744237.75 1864129.75 Load time: 03/26/2013 03:33:52 PM Read time: 03/26/2013 03:33:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3002 Comment: plate_1_repeat2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 49680680 cps Min counts: 9872 counts Well list: r0(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19744.00 49680680.00 44733612.00 3911664.00 2859616.00 13049019.00 21862892.00 28175838.00 B 21593.00 32306292.00 3103925.00 6034421.00 22841790.00 4235418.00 25013326.00 4141827.00 C 24443.00 19710424.00 1389354.00 3586749.00 10326364.00 500515.00 5506519.00 6542308.00 D 23854.00 12726627.00 1477836.00 27010032.00 18175524.00 3389690.00 4704991.00 2401220.00 E 7223962.00 6620720.00 4003721.00 6585567.00 2857865.00 11460303.00 1508358.00 F 3632510.00 23708904.00 12399992.00 6697585.00 27282964.00 9321909.00 12144639.00 G 2389323.00 5101889.00 45315856.00 2980561.00 2044485.00 26607154.00 1032177.00 H 1269091.00 34166828.00 15831630.00 4686104.00 13158035.00 5790214.00 1740773.00 Well list: r1(a2,a3,a4,a5,a6,a7,a8,b2:h8) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49658271.50 44711203.50 3889255.50 2837207.50 13026610.50 21840483.50 28153429.50 B 32283883.50 3081516.50 6012012.50 22819381.50 4213009.50 24990917.50 4119418.50 C 19688015.50 1366945.50 3564340.50 10303955.50 478106.50 5484110.50 6519899.50 D 12704218.50 1455427.50 26987623.50 18153115.50 3367281.50 4682582.50 2378811.50 E 7201553.50 6598311.50 3981312.50 6563158.50 2835456.50 11437894.50 1485949.50 F 3610101.50 23686495.50 12377583.50 6675176.50 27260555.50 9299500.50 12122230.50 G 2366914.50 5079480.50 45293447.50 2958152.50 2022076.50 26584745.50 1009768.50 H 1246682.50 34144419.50 15809221.50 4663695.50 13135626.50 5767805.50 1718364.50 Load time: 03/26/2013 03:35:19 PM Read time: 03/26/2013 03:35:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3003 Comment: plate_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 62543172 cps Min counts: 12340 counts Well list: r0(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24680.00 62543172.00 17625460.00 26466106.00 692686.00 10142049.00 16117068.00 15934597.00 B 34560.00 44043608.00 38733180.00 29290356.00 31107368.00 19841970.00 1823565.00 13377563.00 C 25187.00 26543986.00 5196678.00 5716575.00 9004775.00 7885853.00 7251348.00 1829044.00 D 25325.00 16071384.00 5294951.00 10717583.00 10449934.00 863027.00 12762435.00 17844170.00 E 9458645.00 471691.00 10436745.00 7894322.00 7992407.00 2798235.00 1945072.00 F 4779496.00 8148538.00 1051009.00 3328335.00 4514037.00 2627417.00 5374721.00 G 2726001.00 22664400.00 1480234.00 5459107.00 8453382.00 18185934.00 1330391.00 H 1390663.00 23688390.00 2315873.00 1714303.00 739602.00 16744319.00 22438246.00 Well list: r1(a2,a3,a4,a5,a6,a7,a8,b2:h8) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62515734.00 17598022.00 26438668.00 665248.00 10114611.00 16089630.00 15907159.00 B 44016170.00 38705742.00 29262918.00 31079930.00 19814532.00 1796127.00 13350125.00 C 26516548.00 5169240.00 5689137.00 8977337.00 7858415.00 7223910.00 1801606.00 D 16043946.00 5267513.00 10690145.00 10422496.00 835589.00 12734997.00 17816732.00 E 9431207.00 444253.00 10409307.00 7866884.00 7964969.00 2770797.00 1917634.00 F 4752058.00 8121100.00 1023571.00 3300897.00 4486599.00 2599979.00 5347283.00 G 2698563.00 22636962.00 1452796.00 5431669.00 8425944.00 18158496.00 1302953.00 H 1363225.00 23660952.00 2288435.00 1686865.00 712164.00 16716881.00 22410808.00 Load time: 03/26/2013 03:36:31 PM Read time: 03/26/2013 03:36:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3004 Comment: plate_2_repeat Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 62815376 cps Min counts: 11753 counts Well list: r0(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23507.00 62815376.00 17969392.00 25852394.00 677503.00 10288753.00 16535735.00 16721038.00 B 34523.00 43329576.00 39259996.00 29681344.00 31558776.00 20696612.00 1829398.00 13814070.00 C 25470.00 26950426.00 5294315.00 5713324.00 9248598.00 8271485.00 7323602.00 1858767.00 D 25056.00 15881675.00 5327824.00 11921751.00 10155803.00 883438.00 13227320.00 18611472.00 E 9468009.00 457636.00 10454201.00 8027728.00 8083480.00 3228054.00 1909402.00 F 4714156.00 8232795.00 1026921.00 3300971.00 4540528.00 2673656.00 5752200.00 G 2766241.00 22775596.00 1509537.00 5713159.00 8676430.00 18561424.00 1480701.00 H 1395622.00 24096834.00 2461221.00 1687854.00 743055.00 17073860.00 23048720.00 Well list: r1(a2,a3,a4,a5,a6,a7,a8,b2:h8) = cps(f6(a2,a3,a4,a5,a6,a7,a8,b2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62788237.00 17942253.00 25825255.00 650364.00 10261614.00 16508596.00 16693899.00 B 43302437.00 39232857.00 29654205.00 31531637.00 20669473.00 1802259.00 13786931.00 C 26923287.00 5267176.00 5686185.00 9221459.00 8244346.00 7296463.00 1831628.00 D 15854536.00 5300685.00 11894612.00 10128664.00 856299.00 13200181.00 18584333.00 E 9440870.00 430497.00 10427062.00 8000589.00 8056341.00 3200915.00 1882263.00 F 4687017.00 8205656.00 999782.00 3273832.00 4513389.00 2646517.00 5725061.00 G 2739102.00 22748457.00 1482398.00 5686020.00 8649291.00 18534285.00 1453562.00 H 1368483.00 24069695.00 2434082.00 1660715.00 715916.00 17046721.00 23021581.00