Load time: 05/20/2013 02:44:39 PM Read time: 05/20/2013 02:44:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3032 Comment: plate_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 111519792 cps Min counts: 19032 counts Well list: r0(a1:h8,a9:d9) = cps(f6(a1:h8,a9:d9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85843824.00 43443408.00 41249112.00 26863784.00 9831410.00 36950048.00 30580088.00 36948328.00 40972.00 B 69327320.00 37117644.00 41634212.00 24072718.00 6754691.00 26569750.00 34628884.00 38115712.00 560244.00 C 45940036.00 28726446.00 74423200.00 17787390.00 17444574.00 17853400.00 51051376.00 46862144.00 38065.00 D 18369848.00 21987642.00 16649843.00 26822934.00 21456898.00 15789746.00 111519792.00 77861488.00 43201.00 E 11349536.00 14090170.00 25234380.00 15307145.00 11116838.00 19371792.00 25459376.00 35060192.00 F 5820869.00 6969314.00 28483184.00 99125608.00 14451871.00 82165184.00 51478464.00 49958956.00 G 4130190.00 2683971.00 15408165.00 11896915.00 10732220.00 42592580.00 52110408.00 25937718.00 H 1800995.00 1235789.00 51265684.00 22311482.00 21271180.00 38117544.00 46551600.00 12829264.00 Well list: r1(a1:h8) = cps(f6(a1:h8) - avg(f6(a9:d9))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85673203.50 43272787.50 41078491.50 26693163.50 9660789.50 36779427.50 30409467.50 36777707.50 B 69156699.50 36947023.50 41463591.50 23902097.50 6584070.50 26399129.50 34458263.50 37945091.50 C 45769415.50 28555825.50 74252579.50 17616769.50 17273953.50 17682779.50 50880755.50 46691523.50 D 18199227.50 21817021.50 16479222.50 26652313.50 21286277.50 15619125.50 111349171.50 77690867.50 E 11178915.50 13919549.50 25063759.50 15136524.50 10946217.50 19201171.50 25288755.50 34889571.50 F 5650248.50 6798693.50 28312563.50 98954987.50 14281250.50 81994563.50 51307843.50 49788335.50 G 3959569.50 2513350.50 15237544.50 11726294.50 10561599.50 42421959.50 51939787.50 25767097.50 H 1630374.50 1065168.50 51095063.50 22140861.50 21100559.50 37946923.50 46380979.50 12658643.50 Load time: 05/20/2013 02:46:24 PM Read time: 05/20/2013 02:46:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3033 Comment: plate_1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 134064824 cps Min counts: 23712 counts Well list: r0(a1:h8,a9:d9) = cps(f6(a1:h8,a9:d9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 115277056.00 53289336.00 52021616.00 33989804.00 12168468.00 44728564.00 37816108.00 44380960.00 52356.00 B 89522712.00 44550332.00 52354428.00 30088618.00 8359110.00 31667048.00 42679600.00 47940444.00 1075749.00 C 58264672.00 35970540.00 90917176.00 22463646.00 21572940.00 21329480.00 62666600.00 53660372.00 47424.00 D 23288774.00 27119846.00 21433722.00 32747438.00 25974210.00 18865810.00 134064824.00 99138000.00 55052.00 E 14003420.00 17788188.00 31760810.00 18897700.00 13719997.00 23265566.00 32029934.00 43716152.00 F 7288500.00 8805203.00 36206796.00 127517728.00 17976308.00 100825336.00 64942972.00 59710984.00 G 5198550.00 3356552.00 19252918.00 14726961.00 13234155.00 51206156.00 63150184.00 30661588.00 H 2248967.00 1556249.00 60808784.00 26554642.00 25472446.00 45432568.00 55689480.00 15576881.00 Well list: r1(a1:h8) = cps(f6(a1:h8) - avg(f6(a9:d9))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 114969410.75 52981690.75 51713970.75 33682158.75 11860822.75 44420918.75 37508462.75 44073314.75 B 89215066.75 44242686.75 52046782.75 29780972.75 8051464.75 31359402.75 42371954.75 47632798.75 C 57957026.75 35662894.75 90609530.75 22156000.75 21265294.75 21021834.75 62358954.75 53352726.75 D 22981128.75 26812200.75 21126076.75 32439792.75 25666564.75 18558164.75 133757178.75 98830354.75 E 13695774.75 17480542.75 31453164.75 18590054.75 13412351.75 22957920.75 31722288.75 43408506.75 F 6980854.75 8497557.75 35899150.75 127210082.75 17668662.75 100517690.75 64635326.75 59403338.75 G 4890904.75 3048906.75 18945272.75 14419315.75 12926509.75 50898510.75 62842538.75 30353942.75 H 1941321.75 1248603.75 60501138.75 26246996.75 25164800.75 45124922.75 55381834.75 15269235.75 Load time: 05/20/2013 02:48:09 PM Read time: 05/20/2013 02:48:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3034 Comment: plate_2_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 137883952 cps Min counts: 30662 counts Well list: r0(a1:h8,a9:d9) = cps(f6(a1:h8,a9:d9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 100468016.00 47317472.00 20495156.00 48502864.00 40427696.00 18685952.00 53584304.00 10341027.00 63158.00 B 68096240.00 39687912.00 16140665.00 42026572.00 33920088.00 90523192.00 26296020.00 55017868.00 69496.00 C 43895036.00 33692608.00 14707587.00 34697000.00 19363758.00 11864907.00 21249540.00 37390384.00 64649.00 D 15872783.00 24565138.00 39155420.00 19311938.00 21728838.00 18397948.00 10124715.00 90071240.00 61325.00 E 12471491.00 16931464.00 17490700.00 39697832.00 30280290.00 54998816.00 9268143.00 14480869.00 F 5961144.00 8169419.00 31384094.00 137883952.00 38008036.00 67151504.00 16375408.00 17913836.00 G 4227636.00 3473632.00 17151332.00 27619966.00 38308860.00 32665236.00 12399410.00 37032792.00 H 2351554.00 1255375.00 21304864.00 54161972.00 32552712.00 27527060.00 5107930.00 84249.00 Well list: r1(a1:h8) = cps(f6(a1:h8) - avg(f6(a9:d9))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 100403359.00 47252815.00 20430499.00 48438207.00 40363039.00 18621295.00 53519647.00 10276370.00 B 68031583.00 39623255.00 16076008.00 41961915.00 33855431.00 90458535.00 26231363.00 54953211.00 C 43830379.00 33627951.00 14642930.00 34632343.00 19299101.00 11800250.00 21184883.00 37325727.00 D 15808126.00 24500481.00 39090763.00 19247281.00 21664181.00 18333291.00 10060058.00 90006583.00 E 12406834.00 16866807.00 17426043.00 39633175.00 30215633.00 54934159.00 9203486.00 14416212.00 F 5896487.00 8104762.00 31319437.00 137819295.00 37943379.00 67086847.00 16310751.00 17849179.00 G 4162979.00 3408975.00 17086675.00 27555309.00 38244203.00 32600579.00 12334753.00 36968135.00 H 2286897.00 1190718.00 21240207.00 54097315.00 32488055.00 27462403.00 5043273.00 19592.00 Load time: 05/20/2013 02:49:32 PM Read time: 05/20/2013 02:49:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3035 Comment: plate_2_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 149154048 cps Min counts: 30823 counts Well list: r0(a1:h8,a9:d9) = cps(f6(a1:h8,a9:d9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 106908816.00 49677568.00 22201652.00 52014800.00 43343772.00 19941664.00 57907632.00 10869328.00 64470.00 B 71384696.00 43680068.00 17233248.00 44776504.00 35264236.00 98841720.00 28308470.00 60063400.00 71511.00 C 45985280.00 35588340.00 15266810.00 38721844.00 20753430.00 12761819.00 22887232.00 40764024.00 66342.00 D 16544694.00 26733502.00 42560608.00 21169072.00 23210464.00 19345352.00 10861297.00 96580832.00 61647.00 E 13232169.00 18096796.00 18721206.00 43579352.00 32515376.00 58072860.00 9983749.00 15517200.00 F 6322111.00 8785128.00 33554456.00 149154048.00 40645052.00 74801912.00 17546760.00 19478268.00 G 4443736.00 3636070.00 18351162.00 29729142.00 41061344.00 35215736.00 13160870.00 40335260.00 H 2443425.00 1359529.00 22531872.00 57305648.00 35613884.00 30218044.00 5480969.00 88965.00 Well list: r1(a1:h8) = cps(f6(a1:h8) - avg(f6(a9:d9))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 106842823.50 49611575.50 22135659.50 51948807.50 43277779.50 19875671.50 57841639.50 10803335.50 B 71318703.50 43614075.50 17167255.50 44710511.50 35198243.50 98775727.50 28242477.50 59997407.50 C 45919287.50 35522347.50 15200817.50 38655851.50 20687437.50 12695826.50 22821239.50 40698031.50 D 16478701.50 26667509.50 42494615.50 21103079.50 23144471.50 19279359.50 10795304.50 96514839.50 E 13166176.50 18030803.50 18655213.50 43513359.50 32449383.50 58006867.50 9917756.50 15451207.50 F 6256118.50 8719135.50 33488463.50 149088055.50 40579059.50 74735919.50 17480767.50 19412275.50 G 4377743.50 3570077.50 18285169.50 29663149.50 40995351.50 35149743.50 13094877.50 40269267.50 H 2377432.50 1293536.50 22465879.50 57239655.50 35547891.50 30152051.50 5414976.50 22972.50 Load time: 05/20/2013 02:49:32 PM Read time: 05/20/2013 02:49:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3035 Comment: plate_2_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 149154048 cps Min counts: 30823 counts Well list: r0(a1:h8,a9:d9) = cps(f6(a1:h8,a9:d9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 106908816.00 49677568.00 22201652.00 52014800.00 43343772.00 19941664.00 57907632.00 10869328.00 64470.00 B 71384696.00 43680068.00 17233248.00 44776504.00 35264236.00 98841720.00 28308470.00 60063400.00 71511.00 C 45985280.00 35588340.00 15266810.00 38721844.00 20753430.00 12761819.00 22887232.00 40764024.00 66342.00 D 16544694.00 26733502.00 42560608.00 21169072.00 23210464.00 19345352.00 10861297.00 96580832.00 61647.00 E 13232169.00 18096796.00 18721206.00 43579352.00 32515376.00 58072860.00 9983749.00 15517200.00 F 6322111.00 8785128.00 33554456.00 149154048.00 40645052.00 74801912.00 17546760.00 19478268.00 G 4443736.00 3636070.00 18351162.00 29729142.00 41061344.00 35215736.00 13160870.00 40335260.00 H 2443425.00 1359529.00 22531872.00 57305648.00 35613884.00 30218044.00 5480969.00 88965.00 Well list: r1(a1:h8) = cps(f6(a1:h8) - avg(f6(a9:d9))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 106842823.50 49611575.50 22135659.50 51948807.50 43277779.50 19875671.50 57841639.50 10803335.50 B 71318703.50 43614075.50 17167255.50 44710511.50 35198243.50 98775727.50 28242477.50 59997407.50 C 45919287.50 35522347.50 15200817.50 38655851.50 20687437.50 12695826.50 22821239.50 40698031.50 D 16478701.50 26667509.50 42494615.50 21103079.50 23144471.50 19279359.50 10795304.50 96514839.50 E 13166176.50 18030803.50 18655213.50 43513359.50 32449383.50 58006867.50 9917756.50 15451207.50 F 6256118.50 8719135.50 33488463.50 149088055.50 40579059.50 74735919.50 17480767.50 19412275.50 G 4377743.50 3570077.50 18285169.50 29663149.50 40995351.50 35149743.50 13094877.50 40269267.50 H 2377432.50 1293536.50 22465879.50 57239655.50 35547891.50 30152051.50 5414976.50 22972.50