Load time: 09/06/2013 10:36:33 AM Read time: 09/06/2013 10:36:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3233 Comment: plate3IIB_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 45934716 cps Min counts: 8097 counts Well list: r0(a1:e1,a2:c2,a3:h8) = cps(f6(a1:e1,a2:c2,a3:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45934716.00 16383.00 606836.00 1030051.00 970021.00 69280.00 450052.00 70096.00 B 4712169.00 16963.00 692471.00 190811.00 700500.00 951423.00 332491.00 114782.00 C 540643.00 16236.00 1590073.00 2036794.00 368113.00 61131.00 447370.00 54848.00 D 55164.00 756987.00 563243.00 54583.00 1574627.00 853525.00 25614.00 E 16195.00 16761.00 871173.00 55333.00 160305.00 25366.00 119513.00 F 586643.00 1080966.00 85240.00 742947.00 22137.00 33027.00 G 1141316.00 42106.00 184298.00 139247.00 139240.00 387651.00 H 134740.00 30164.00 80897.00 152961.00 105629.00 87881.00 Well list: r1(a1:e1) = cps(f6(a1:e1) - avg(f6(a2:c2))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45918188.67 B 4695641.67 C 524115.67 D 38636.67 E -332.33 F G H Load time: 09/06/2013 10:38:36 AM Read time: 09/06/2013 10:38:36 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3234 Comment: plate3IIB_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 47949132 cps Min counts: 10321 counts Well list: r0(a1:e1,a2:c2,a3:h8) = cps(f6(a1:e1,a2:c2,a3:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47949132.00 20643.00 780034.00 1180825.00 1101556.00 79269.00 497881.00 76922.00 B 5537635.00 22930.00 802638.00 225832.00 798426.00 1017049.00 373182.00 128974.00 C 644356.00 22864.00 1762733.00 2275653.00 424256.00 72453.00 487992.00 64067.00 D 66906.00 873564.00 594810.00 62007.00 1406204.00 938681.00 32449.00 E 22655.00 23846.00 933051.00 62655.00 180824.00 33499.00 138765.00 F 646981.00 1211280.00 97796.00 835593.00 28552.00 40728.00 G 1333397.00 44052.00 205012.00 159358.00 157783.00 383349.00 H 199277.00 35490.00 99751.00 168546.00 116618.00 97178.00 Well list: r1(a1:e1) = cps(f6(a1:e1) - avg(f6(a2:c2))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47926986.33 B 5515489.33 C 622210.33 D 44760.33 E 509.33 F G H Load time: 09/06/2013 10:42:20 AM Read time: 09/06/2013 10:42:20 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3235 Comment: plate1_1-10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 46677520 cps Min counts: 9525 counts Well list: r0(a1:d1,e1,a2:c2,a3:h12) = cps(f6(a1:d1,e1,a2:c2,a3:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 46677520.00 19050.00 785526.00 1184902.00 1088806.00 77800.00 479968.00 71118.00 30106.00 164781.00 144660.00 160087.00 B 5471148.00 23155.00 784079.00 239295.00 774164.00 975384.00 358435.00 117579.00 28323.00 103935.00 1007553.00 131534.00 C 637206.00 21967.00 1762010.00 2294724.00 414423.00 68389.00 475229.00 64765.00 92168.00 111658.00 506302.00 119881.00 D 66949.00 859038.00 595470.00 61478.00 1368848.00 920736.00 32160.00 84385.00 92846.00 273204.00 58205.00 E 21540.00 23343.00 919834.00 61439.00 178166.00 32829.00 136742.00 563320.00 202517.00 98746.00 63300.00 F 636510.00 1212614.00 94947.00 837517.00 28352.00 39872.00 258811.00 551199.00 149819.00 59174.00 G 1323192.00 43984.00 204669.00 158179.00 153988.00 379260.00 41187.00 152346.00 431731.00 119742.00 H 201126.00 35118.00 97589.00 161564.00 108823.00 94625.00 29060.00 120044.00 136513.00 56788.00 Well list: r1(a1:d1,e1) = cps(f6(a1:d1,e1) - avg(f6(a2:c2))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 46656129.33 B 5449757.33 C 615815.33 D 45558.33 E 149.33 F G H