Load time: 03/22/2013 03:39:01 PM Read time: 03/22/2013 03:39:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2992 Comment: plate_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 75092040 cps Min counts: 14123 counts Well list: r0(a2:h8,a1:d1) = cps(f6(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36718.00 75092040.00 4119730.00 15029497.00 216067.00 5797911.00 7780349.00 6683881.00 B 28246.00 43519620.00 18769014.00 11642339.00 16478066.00 7913053.00 535576.00 4312923.00 C 28717.00 26129822.00 1933230.00 1922633.00 4285147.00 2448603.00 1682024.00 605198.00 D 28573.00 16600052.00 1855348.00 2977464.00 4459213.00 380895.00 6160827.00 4207714.00 E 10256847.00 103195.00 3630084.00 3331939.00 2882554.00 630322.00 471444.00 F 5499157.00 2524971.00 346038.00 778870.00 1713359.00 1167267.00 1325001.00 G 3529559.00 6516956.00 716833.00 1600796.00 4538478.00 4962725.00 323379.00 H 2299770.00 11045220.00 958650.00 755422.00 215254.00 7439669.00 7866238.00 Well list: r1(a2:h8) = cps(f6(a2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 75061476.50 4089166.50 14998933.50 185503.50 5767347.50 7749785.50 6653317.50 B 43489056.50 18738450.50 11611775.50 16447502.50 7882489.50 505012.50 4282359.50 C 26099258.50 1902666.50 1892069.50 4254583.50 2418039.50 1651460.50 574634.50 D 16569488.50 1824784.50 2946900.50 4428649.50 350331.50 6130263.50 4177150.50 E 10226283.50 72631.50 3599520.50 3301375.50 2851990.50 599758.50 440880.50 F 5468593.50 2494407.50 315474.50 748306.50 1682795.50 1136703.50 1294437.50 G 3498995.50 6486392.50 686269.50 1570232.50 4507914.50 4932161.50 292815.50 H 2269206.50 11014656.50 928086.50 724858.50 184690.50 7409105.50 7835674.50 Load time: 03/22/2013 03:40:36 PM Read time: 03/22/2013 03:40:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2993 Comment: plate_2_repeat Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 74717416 cps Min counts: 14392 counts Well list: r0(a2:h8,a1:d1) = cps(f6(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36693.00 74717416.00 4112026.00 15155466.00 207967.00 5778917.00 7692809.00 6594813.00 B 28784.00 42824656.00 18140220.00 12145940.00 16592766.00 7771436.00 525358.00 4276168.00 C 29027.00 25350424.00 1916150.00 1895912.00 4179995.00 2442582.00 1703008.00 589645.00 D 29211.00 15895668.00 1845243.00 2940155.00 4439089.00 375412.00 6107171.00 4081474.00 E 9779931.00 99406.00 3863478.00 3331827.00 3047477.00 624784.00 467471.00 F 5717481.00 2481968.00 326787.00 764685.00 1744434.00 1113316.00 1307875.00 G 3579810.00 6361273.00 710070.00 1581107.00 4526391.00 4940087.00 301659.00 H 2285678.00 10552035.00 981404.00 748143.00 209780.00 7381694.00 7658519.00 Well list: r1(a2:h8) = cps(f6(a2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74686487.25 4081097.25 15124537.25 177038.25 5747988.25 7661880.25 6563884.25 B 42793727.25 18109291.25 12115011.25 16561837.25 7740507.25 494429.25 4245239.25 C 25319495.25 1885221.25 1864983.25 4149066.25 2411653.25 1672079.25 558716.25 D 15864739.25 1814314.25 2909226.25 4408160.25 344483.25 6076242.25 4050545.25 E 9749002.25 68477.25 3832549.25 3300898.25 3016548.25 593855.25 436542.25 F 5686552.25 2451039.25 295858.25 733756.25 1713505.25 1082387.25 1276946.25 G 3548881.25 6330344.25 679141.25 1550178.25 4495462.25 4909158.25 270730.25 H 2254749.25 10521106.25 950475.25 717214.25 178851.25 7350765.25 7627590.25 Load time: 03/22/2013 03:41:59 PM Read time: 03/22/2013 03:41:59 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2994 Comment: plate_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 69717008 cps Min counts: 11607 counts Well list: r0(a2:h8,a1:d1) = cps(f6(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23215.00 69717008.00 13924814.00 1849215.00 990256.00 4290451.00 4597196.00 7553002.00 B 24897.00 50489628.00 689523.00 2794295.00 10623775.00 1912194.00 6516528.00 1889441.00 C 109451.00 30567132.00 461761.00 733986.00 4699114.00 121011.00 1278249.00 2181093.00 D 41697.00 17721078.00 250861.00 5314235.00 6983845.00 819919.00 794784.00 1028752.00 E 9248274.00 1539779.00 1370668.00 2910744.00 933832.00 3504719.00 571123.00 F 5350953.00 8221114.00 3146720.00 2699983.00 13453061.00 2760028.00 7057675.00 G 3211546.00 1350749.00 12400185.00 713954.00 415636.00 14110457.00 293876.00 H 2337332.00 8086445.00 2584482.00 1109676.00 4964029.00 1607988.00 511536.00 Well list: r1(a2:h8) = cps(f6(a2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 69667193.00 13874999.00 1799400.00 940441.00 4240636.00 4547381.00 7503187.00 B 50439813.00 639708.00 2744480.00 10573960.00 1862379.00 6466713.00 1839626.00 C 30517317.00 411946.00 684171.00 4649299.00 71196.00 1228434.00 2131278.00 D 17671263.00 201046.00 5264420.00 6934030.00 770104.00 744969.00 978937.00 E 9198459.00 1489964.00 1320853.00 2860929.00 884017.00 3454904.00 521308.00 F 5301138.00 8171299.00 3096905.00 2650168.00 13403246.00 2710213.00 7007860.00 G 3161731.00 1300934.00 12350370.00 664139.00 365821.00 14060642.00 244061.00 H 2287517.00 8036630.00 2534667.00 1059861.00 4914214.00 1558173.00 461721.00 Load time: 03/22/2013 03:43:31 PM Read time: 03/22/2013 03:43:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2995 Comment: plate_1_repeat Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 68983920 cps Min counts: 12234 counts Well list: r0(a2:h8,a1:d1) = cps(f6(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24488.00 68983920.00 14318403.00 1770040.00 979821.00 4351914.00 4485544.00 7249229.00 B 24468.00 48885196.00 687970.00 2749083.00 9939495.00 1899597.00 6241040.00 1899706.00 C 105594.00 30493158.00 437481.00 690124.00 4760778.00 119372.00 1252821.00 2183270.00 D 46643.00 16048315.00 236584.00 5052304.00 6657087.00 811707.00 772064.00 1027064.00 E 8752389.00 1442469.00 1320540.00 2947119.00 915774.00 3532669.00 581072.00 F 5121904.00 7791652.00 3049772.00 2705800.00 13180755.00 2732175.00 7012162.00 G 3114948.00 1367240.00 11821791.00 686418.00 412221.00 14353353.00 298054.00 H 2245932.00 7978028.00 2569332.00 1084792.00 4957023.00 1614119.00 508239.00 Well list: r1(a2:h8) = cps(f6(a2:h8) - avg(f6(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 68933621.75 14268104.75 1719741.75 929522.75 4301615.75 4435245.75 7198930.75 B 48834897.75 637671.75 2698784.75 9889196.75 1849298.75 6190741.75 1849407.75 C 30442859.75 387182.75 639825.75 4710479.75 69073.75 1202522.75 2132971.75 D 15998016.75 186285.75 5002005.75 6606788.75 761408.75 721765.75 976765.75 E 8702090.75 1392170.75 1270241.75 2896820.75 865475.75 3482370.75 530773.75 F 5071605.75 7741353.75 2999473.75 2655501.75 13130456.75 2681876.75 6961863.75 G 3064649.75 1316941.75 11771492.75 636119.75 361922.75 14303054.75 247755.75 H 2195633.75 7927729.75 2519033.75 1034493.75 4906724.75 1563820.75 457940.75