Load time: 06/12/2013 03:08:37 PM Read time: 06/12/2013 03:08:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3058 Comment: plate2IIA_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 67402384 cps Min counts: 41138 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40300444.00 36698932.00 38795656.00 31248192.00 18316018.00 33963628.00 19329808.00 86160.00 B 33469574.00 19343468.00 33396352.00 54224868.00 35518272.00 29329314.00 51100676.00 110713.00 C 27352534.00 17561510.00 22697448.00 35753440.00 27846694.00 39498328.00 37922696.00 90193.00 D 19395932.00 20155852.00 28136890.00 34329364.00 18187184.00 25865032.00 29135544.00 82277.00 E 12703612.00 31901212.00 29757006.00 37082964.00 30514258.00 38178040.00 53457996.00 F 6192934.00 23150894.00 62180620.00 28833922.00 28198858.00 67402384.00 38769128.00 G 3199946.00 30598946.00 35364784.00 43608080.00 42263756.00 21883986.00 40398892.00 H 1208054.00 34149192.00 28866426.00 29018384.00 19074344.00 40626332.00 28848592.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40208108.25 36606596.25 38703320.25 31155856.25 18223682.25 33871292.25 19237472.25 B 33377238.25 19251132.25 33304016.25 54132532.25 35425936.25 29236978.25 51008340.25 C 27260198.25 17469174.25 22605112.25 35661104.25 27754358.25 39405992.25 37830360.25 D 19303596.25 20063516.25 28044554.25 34237028.25 18094848.25 25772696.25 29043208.25 E 12611276.25 31808876.25 29664670.25 36990628.25 30421922.25 38085704.25 53365660.25 F 6100598.25 23058558.25 62088284.25 28741586.25 28106522.25 67310048.25 38676792.25 G 3107610.25 30506610.25 35272448.25 43515744.25 42171420.25 21791650.25 40306556.25 H 1115718.25 34056856.25 28774090.25 28926048.25 18982008.25 40533996.25 28756256.25 Load time: 06/12/2013 03:09:56 PM Read time: 06/12/2013 03:09:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3059 Comment: plate2IIA_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 71378280 cps Min counts: 48929 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49069536.00 46369408.00 48088560.00 40780780.00 22120602.00 41268408.00 25214342.00 98455.00 B 39417908.00 21638214.00 38421968.00 58974584.00 35929836.00 36901952.00 63306312.00 156809.00 C 32665234.00 18645570.00 26604482.00 38616360.00 34184484.00 58791136.00 46513412.00 109625.00 D 24570460.00 23441452.00 31748106.00 36999996.00 19887208.00 28709822.00 36411076.00 97858.00 E 15859464.00 40495760.00 35391584.00 40810132.00 33099736.00 35838996.00 71103360.00 F 7384921.00 29348832.00 71378280.00 37560228.00 33079044.00 51100676.00 46290992.00 G 3724879.00 37374540.00 43400648.00 54069744.00 50946532.00 26327414.00 50528188.00 H 1508358.00 42408264.00 34849324.00 34295252.00 23906692.00 45841876.00 35937968.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48953849.25 46253721.25 47972873.25 40665093.25 22004915.25 41152721.25 25098655.25 B 39302221.25 21522527.25 38306281.25 58858897.25 35814149.25 36786265.25 63190625.25 C 32549547.25 18529883.25 26488795.25 38500673.25 34068797.25 58675449.25 46397725.25 D 24454773.25 23325765.25 31632419.25 36884309.25 19771521.25 28594135.25 36295389.25 E 15743777.25 40380073.25 35275897.25 40694445.25 32984049.25 35723309.25 70987673.25 F 7269234.25 29233145.25 71262593.25 37444541.25 32963357.25 50984989.25 46175305.25 G 3609192.25 37258853.25 43284961.25 53954057.25 50830845.25 26211727.25 50412501.25 H 1392671.25 42292577.25 34733637.25 34179565.25 23791005.25 45726189.25 35822281.25 Load time: 06/12/2013 03:11:31 PM Read time: 06/12/2013 03:11:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3060 Comment: plate2IIB_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 79158144 cps Min counts: 49815 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48894232.00 47266760.00 39417912.00 35824436.00 22409742.00 48464372.00 50119828.00 158696.00 B 40672124.00 72899064.00 35010716.00 48639580.00 19053740.00 50900792.00 64578420.00 587819.00 C 32430360.00 75917936.00 56582640.00 40376292.00 72334416.00 46033280.00 30629644.00 229495.00 D 24004290.00 47609780.00 24398276.00 29102426.00 36268008.00 43258744.00 25173560.00 99631.00 E 16481145.00 36705720.00 37353440.00 25798626.00 44580360.00 68078720.00 37922696.00 F 7989430.00 52230420.00 46054644.00 21203000.00 36755008.00 79158144.00 41951056.00 G 3965202.00 69631392.00 62400592.00 68822424.00 33185532.00 50403064.00 18698274.00 H 1499542.00 31123226.00 19839988.00 20751800.00 39713720.00 32057100.00 40770308.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48625321.75 46997849.75 39149001.75 35555525.75 22140831.75 48195461.75 49850917.75 B 40403213.75 72630153.75 34741805.75 48370669.75 18784829.75 50631881.75 64309509.75 C 32161449.75 75649025.75 56313729.75 40107381.75 72065505.75 45764369.75 30360733.75 D 23735379.75 47340869.75 24129365.75 28833515.75 35999097.75 42989833.75 24904649.75 E 16212234.75 36436809.75 37084529.75 25529715.75 44311449.75 67809809.75 37653785.75 F 7720519.75 51961509.75 45785733.75 20934089.75 36486097.75 78889233.75 41682145.75 G 3696291.75 69362481.75 62131681.75 68553513.75 32916621.75 50134153.75 18429363.75 H 1230631.75 30854315.75 19571077.75 20482889.75 39444809.75 31788189.75 40501397.75 Load time: 06/12/2013 03:12:54 PM Read time: 06/12/2013 03:12:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3061 Comment: plate2IIB_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 78898504 cps Min counts: 47339 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45581276.00 45831284.00 35660284.00 32110266.00 20325648.00 48437752.00 53368148.00 124742.00 B 38697308.00 57886528.00 35986848.00 48547360.00 15962474.00 41551396.00 61711716.00 574404.00 C 30150330.00 78898504.00 55124848.00 39064856.00 55193840.00 32519370.00 27584456.00 255483.00 D 22300198.00 45565576.00 26171174.00 34218376.00 32097280.00 41836088.00 26693048.00 94678.00 E 15719704.00 31912756.00 38292224.00 22898458.00 39211516.00 58513776.00 33497822.00 F 7343656.00 49877344.00 44500384.00 18893652.00 31659470.00 73093728.00 36289560.00 G 3600006.00 69515528.00 58440544.00 59868612.00 31240816.00 47960716.00 17788984.00 H 1378880.00 29065130.00 18602312.00 19517540.00 34744076.00 29047054.00 36466276.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45318949.25 45568957.25 35397957.25 31847939.25 20063321.25 48175425.25 53105821.25 B 38434981.25 57624201.25 35724521.25 48285033.25 15700147.25 41289069.25 61449389.25 C 29888003.25 78636177.25 54862521.25 38802529.25 54931513.25 32257043.25 27322129.25 D 22037871.25 45303249.25 25908847.25 33956049.25 31834953.25 41573761.25 26430721.25 E 15457377.25 31650429.25 38029897.25 22636131.25 38949189.25 58251449.25 33235495.25 F 7081329.25 49615017.25 44238057.25 18631325.25 31397143.25 72831401.25 36027233.25 G 3337679.25 69253201.25 58178217.25 59606285.25 30978489.25 47698389.25 17526657.25 H 1116553.25 28802803.25 18339985.25 19255213.25 34481749.25 28784727.25 36203949.25