Load time: 06/03/2013 02:40:33 PM Read time: 06/03/2013 02:40:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3051 Comment: plate3A_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 51057944 cps Min counts: 36645 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39899364.00 17549474.00 9691453.00 3284907.00 13542110.00 15418342.00 13258250.00 73290.00 B 34385872.00 17616466.00 9545773.00 2460915.00 10128202.00 13499965.00 16306781.00 76259.00 C 27672928.00 33322048.00 6524562.00 7499909.00 6821387.00 20637424.00 18506900.00 79263.00 D 19992894.00 8197258.00 11646608.00 9230315.00 6216991.00 50447912.00 33368276.00 74668.00 E 11365135.00 10355461.00 6721518.00 4701953.00 7159911.00 10535649.00 16457228.00 F 7159201.00 11695340.00 51057944.00 5620763.00 31600232.00 29119508.00 21323176.00 G 3210675.00 6122574.00 4434501.00 3997391.00 16863482.00 21931146.00 10856692.00 H 1662320.00 19380778.00 8461221.00 8882747.00 15155466.00 21859882.00 4389576.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39823494.00 17473604.00 9615583.00 3209037.00 13466240.00 15342472.00 13182380.00 B 34310002.00 17540596.00 9469903.00 2385045.00 10052332.00 13424095.00 16230911.00 C 27597058.00 33246178.00 6448692.00 7424039.00 6745517.00 20561554.00 18431030.00 D 19917024.00 8121388.00 11570738.00 9154445.00 6141121.00 50372042.00 33292406.00 E 11289265.00 10279591.00 6645648.00 4626083.00 7084041.00 10459779.00 16381358.00 F 7083331.00 11619470.00 50982074.00 5544893.00 31524362.00 29043638.00 21247306.00 G 3134805.00 6046704.00 4358631.00 3921521.00 16787612.00 21855276.00 10780822.00 H 1586450.00 19304908.00 8385351.00 8806877.00 15079596.00 21784012.00 4313706.00 Load time: 06/03/2013 02:42:05 PM Read time: 06/03/2013 02:42:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3052 Comment: plate3A_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 62151412 cps Min counts: 47674 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52196076.00 21709226.00 11887650.00 3876420.00 16155775.00 19544452.00 16196981.00 95348.00 B 43225760.00 21042810.00 11733026.00 2956361.00 12448607.00 16490731.00 20211790.00 101085.00 C 34532468.00 41451588.00 7710743.00 9245581.00 8268641.00 25199134.00 22863500.00 101978.00 D 24769804.00 9962197.00 14607447.00 11428788.00 7478923.00 62151412.00 40537120.00 96984.00 E 13988367.00 12522249.00 8276227.00 5738560.00 8648731.00 12937575.00 19247318.00 F 8822329.00 13999715.00 60734348.00 6942434.00 38049944.00 34533972.00 25991222.00 G 3969247.00 7416106.00 5466476.00 4898777.00 19540122.00 26589330.00 12937787.00 H 2043779.00 22601802.00 10342374.00 10725404.00 17724638.00 25871778.00 5301812.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52097227.25 21610377.25 11788801.25 3777571.25 16056926.25 19445603.25 16098132.25 B 43126911.25 20943961.25 11634177.25 2857512.25 12349758.25 16391882.25 20112941.25 C 34433619.25 41352739.25 7611894.25 9146732.25 8169792.25 25100285.25 22764651.25 D 24670955.25 9863348.25 14508598.25 11329939.25 7380074.25 62052563.25 40438271.25 E 13889518.25 12423400.25 8177378.25 5639711.25 8549882.25 12838726.25 19148469.25 F 8723480.25 13900866.25 60635499.25 6843585.25 37951095.25 34435123.25 25892373.25 G 3870398.25 7317257.25 5367627.25 4799928.25 19441273.25 26490481.25 12838938.25 H 1944930.25 22502953.25 10243525.25 10626555.25 17625789.25 25772929.25 5202963.25 Load time: 06/03/2013 02:44:00 PM Read time: 06/03/2013 02:44:00 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3053 Comment: plate3B_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 67442464 cps Min counts: 50306 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48801048.00 6273594.00 18295756.00 13403087.00 6770824.00 22219674.00 3734535.00 103699.00 B 42002112.00 5252139.00 17666648.00 12614181.00 35779224.00 11691724.00 23262838.00 105118.00 C 33497822.00 5076895.00 16067157.00 7354678.00 3814740.00 9659024.00 15305669.00 100612.00 D 23817748.00 19682564.00 8656559.00 11264657.00 6969374.00 3350659.00 36651484.00 104689.00 E 16216836.00 7052972.00 18906704.00 12974157.00 25547048.00 3552240.00 4957116.00 F 7663843.00 12525015.00 67442464.00 14962638.00 27605560.00 6107453.00 6611952.00 G 2595790.00 6638826.00 12221112.00 15325467.00 14311948.00 4076695.00 16438483.00 H 1847008.00 115246.00 22831934.00 11697926.00 11685183.00 911314.00 113019.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48697518.50 6170064.50 18192226.50 13299557.50 6667294.50 22116144.50 3631005.50 B 41898582.50 5148609.50 17563118.50 12510651.50 35675694.50 11588194.50 23159308.50 C 33394292.50 4973365.50 15963627.50 7251148.50 3711210.50 9555494.50 15202139.50 D 23714218.50 19579034.50 8553029.50 11161127.50 6865844.50 3247129.50 36547954.50 E 16113306.50 6949442.50 18803174.50 12870627.50 25443518.50 3448710.50 4853586.50 F 7560313.50 12421485.50 67338934.50 14859108.50 27502030.50 6003923.50 6508422.50 G 2492260.50 6535296.50 12117582.50 15221937.50 14208418.50 3973165.50 16334953.50 H 1743478.50 11716.50 22728404.50 11594396.50 11581653.50 807784.50 9489.50 Load time: 06/03/2013 02:45:34 PM Read time: 06/03/2013 02:45:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3054 Comment: plate3B_11mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 60660084 cps Min counts: 48564 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44806836.00 5911204.00 17640344.00 12638484.00 6336696.00 20605816.00 3512411.00 97129.00 B 38881164.00 4887827.00 16197312.00 11594215.00 32977352.00 11272016.00 21834628.00 97831.00 C 30977480.00 4676441.00 15028926.00 6831307.00 3524748.00 9177172.00 14426395.00 97306.00 D 22026692.00 18241938.00 8201323.00 10521265.00 6468682.00 3176653.00 34196264.00 98185.00 E 15288864.00 6621825.00 17931236.00 12010135.00 24180514.00 3305507.00 4613387.00 F 7269943.00 11601500.00 60660084.00 14127533.00 25898790.00 5641615.00 6085447.00 G 2395886.00 6236431.00 11195389.00 14399178.00 13011799.00 3763912.00 15320142.00 H 1746383.00 107122.00 21388098.00 10756360.00 10911760.00 844371.00 105023.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44709223.25 5813591.25 17542731.25 12540871.25 6239083.25 20508203.25 3414798.25 B 38783551.25 4790214.25 16099699.25 11496602.25 32879739.25 11174403.25 21737015.25 C 30879867.25 4578828.25 14931313.25 6733694.25 3427135.25 9079559.25 14328782.25 D 21929079.25 18144325.25 8103710.25 10423652.25 6371069.25 3079040.25 34098651.25 E 15191251.25 6524212.25 17833623.25 11912522.25 24082901.25 3207894.25 4515774.25 F 7172330.25 11503887.25 60562471.25 14029920.25 25801177.25 5544002.25 5987834.25 G 2298273.25 6138818.25 11097776.25 14301565.25 12914186.25 3666299.25 15222529.25 H 1648770.25 9509.25 21290485.25 10658747.25 10814147.25 746758.25 7410.25 Load time: 06/10/2013 01:03:32 PM Read time: 06/10/2013 01:03:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3056 Comment: plate3B_11mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 2399 cps Min counts: 1037 counts Well list: r0(a1:h7,a8:d8) = cps(f6(a1:h7,a8:d8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2232.00 2325.00 2166.00 2125.00 2131.00 2284.00 2164.00 2253.00 B 2379.00 2139.00 2399.00 2149.00 2187.00 2269.00 2205.00 2265.00 C 2211.00 2158.00 2234.00 2075.00 2248.00 2265.00 2189.00 2193.00 D 2133.00 2197.00 2265.00 2294.00 2137.00 2244.00 2139.00 2255.00 E 2236.00 2172.00 2079.00 2284.00 2199.00 2205.00 2215.00 F 2323.00 2205.00 2248.00 2153.00 2244.00 2263.00 2170.00 G 2172.00 2217.00 2193.00 2248.00 2199.00 2253.00 2170.00 H 2209.00 2222.00 2232.00 2248.00 2288.00 2220.00 2085.00 Well list: r1(a1:h7) = cps(f6(a1:h7) - avg(f6(a8:d8))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A -9.50 83.50 -75.50 -116.50 -110.50 42.50 -77.50 B 137.50 -102.50 157.50 -92.50 -54.50 27.50 -36.50 C -30.50 -83.50 -7.50 -166.50 6.50 23.50 -52.50 D -108.50 -44.50 23.50 52.50 -104.50 2.50 -102.50 E -5.50 -69.50 -162.50 42.50 -42.50 -36.50 -26.50 F 81.50 -36.50 6.50 -88.50 2.50 21.50 -71.50 G -69.50 -24.50 -48.50 6.50 -42.50 11.50 -71.50 H -32.50 -19.50 -9.50 6.50 46.50 -21.50 -156.50