Load time: 06/03/2013 02:33:48 PM Read time: 06/03/2013 02:33:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3049 Comment: plate4I_plate4II_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 40218772 cps Min counts: 36644 counts Well list: r0(a1:h11,a12:d12) = cps(f6(a1:h11,a12:d12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40218772.00 16542624.00 10885873.00 23762134.00 10716861.00 16161367.00 8897882.00 7788975.00 5965893.00 3786029.00 7543599.00 73362.00 B 34779088.00 7437718.00 12242788.00 14273603.00 15533596.00 6262261.00 8117538.00 15456469.00 12469336.00 6811084.00 4556270.00 73288.00 C 27795990.00 5382665.00 19132732.00 19343468.00 18524174.00 3273163.00 21477794.00 22846390.00 13115639.00 4480785.00 15233120.00 73482.00 D 21257508.00 8419936.00 6263940.00 26021040.00 17015304.00 5436264.00 23886552.00 17644658.00 16221476.00 8562160.00 8235100.00 76742.00 E 13405803.00 22034026.00 9505993.00 5501711.00 29912872.00 79003.00 15958623.00 16534355.00 8875894.00 12669144.00 8479117.00 F 7657706.00 24308616.00 1973090.00 21401940.00 7336598.00 82434.00 27642090.00 14377792.00 3559344.00 14960384.00 5822706.00 G 3141042.00 1989343.00 9734238.00 5389756.00 16649493.00 85207.00 22165076.00 10342644.00 2573447.00 4236051.00 83850.00 H 1671889.00 4812179.00 351950.00 18996744.00 8160935.00 85306.00 12093755.00 13918220.00 3769256.00 2968600.00 82102.00 Well list: r1(a1:h11) = cps(f6(a1:h11) - avg(f6(a12:d12))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40144553.50 16468405.50 10811654.50 23687915.50 10642642.50 16087148.50 8823663.50 7714756.50 5891674.50 3711810.50 7469380.50 B 34704869.50 7363499.50 12168569.50 14199384.50 15459377.50 6188042.50 8043319.50 15382250.50 12395117.50 6736865.50 4482051.50 C 27721771.50 5308446.50 19058513.50 19269249.50 18449955.50 3198944.50 21403575.50 22772171.50 13041420.50 4406566.50 15158901.50 D 21183289.50 8345717.50 6189721.50 25946821.50 16941085.50 5362045.50 23812333.50 17570439.50 16147257.50 8487941.50 8160881.50 E 13331584.50 21959807.50 9431774.50 5427492.50 29838653.50 4784.50 15884404.50 16460136.50 8801675.50 12594925.50 8404898.50 F 7583487.50 24234397.50 1898871.50 21327721.50 7262379.50 8215.50 27567871.50 14303573.50 3485125.50 14886165.50 5748487.50 G 3066823.50 1915124.50 9660019.50 5315537.50 16575274.50 10988.50 22090857.50 10268425.50 2499228.50 4161832.50 9631.50 H 1597670.50 4737960.50 277731.50 18922525.50 8086716.50 11087.50 12019536.50 13844001.50 3695037.50 2894381.50 7883.50 Load time: 06/03/2013 02:35:39 PM Read time: 06/03/2013 02:35:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #3050 Comment: plate4I_plate4II_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 52639116 cps Min counts: 46602 counts Well list: r0(a1:h11,a12:d12) = cps(f6(a1:h11,a12:d12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52639116.00 20775698.00 13729488.00 30188168.00 12879011.00 19595592.00 10980438.00 9682469.00 7408078.00 4753650.00 9410763.00 94697.00 B 44622964.00 9296254.00 15254782.00 17676484.00 18718116.00 7650621.00 9796951.00 18652580.00 15490855.00 8166476.00 5538331.00 93920.00 C 34947516.00 6635663.00 24051566.00 24109276.00 22318382.00 4025895.00 25966564.00 28339800.00 16279026.00 5457643.00 18286482.00 93205.00 D 26757834.00 10264673.00 7693777.00 32347104.00 20907024.00 6695947.00 30134306.00 21660060.00 19729530.00 10468677.00 10035245.00 97864.00 E 16994902.00 26968738.00 11837835.00 6791330.00 36731192.00 96897.00 18997196.00 20110404.00 10941843.00 15270630.00 10373866.00 F 9535224.00 30093180.00 2482713.00 25492898.00 8826449.00 98996.00 31996362.00 17190704.00 4341822.00 18020790.00 7130583.00 G 3962668.00 2457966.00 11815105.00 6623924.00 20208190.00 103087.00 25978464.00 12519485.00 3168398.00 5174625.00 98854.00 H 2089821.00 5871720.00 428310.00 23001300.00 9861824.00 101273.00 14318917.00 16198964.00 4470992.00 3480425.00 99298.00 Well list: r1(a1:h11) = cps(f6(a1:h11) - avg(f6(a12:d12))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52544194.50 20680776.50 13634566.50 30093246.50 12784089.50 19500670.50 10885516.50 9587547.50 7313156.50 4658728.50 9315841.50 B 44528042.50 9201332.50 15159860.50 17581562.50 18623194.50 7555699.50 9702029.50 18557658.50 15395933.50 8071554.50 5443409.50 C 34852594.50 6540741.50 23956644.50 24014354.50 22223460.50 3930973.50 25871642.50 28244878.50 16184104.50 5362721.50 18191560.50 D 26662912.50 10169751.50 7598855.50 32252182.50 20812102.50 6601025.50 30039384.50 21565138.50 19634608.50 10373755.50 9940323.50 E 16899980.50 26873816.50 11742913.50 6696408.50 36636270.50 1975.50 18902274.50 20015482.50 10846921.50 15175708.50 10278944.50 F 9440302.50 29998258.50 2387791.50 25397976.50 8731527.50 4074.50 31901440.50 17095782.50 4246900.50 17925868.50 7035661.50 G 3867746.50 2363044.50 11720183.50 6529002.50 20113268.50 8165.50 25883542.50 12424563.50 3073476.50 5079703.50 3932.50 H 1994899.50 5776798.50 333388.50 22906378.50 9766902.50 6351.50 14223995.50 16104042.50 4376070.50 3385503.50 4376.50