Load time: 10/01/2010 09:35:05 AM Read time: 10/01/2010 09:35:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1065 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28343532 cps Min counts: 8846 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27477336.00 19996018.00 28343532.00 10450925.00 4495628.00 2410059.00 B 3976559.00 2269987.00 3159415.00 16516387.00 7075123.00 3427292.00 C 682733.00 332958.00 586214.00 19204764.00 8384186.00 3961187.00 D 143025.00 83910.00 125352.00 18176346.00 7922072.00 3993493.00 E 24700.00 46678.00 37041.00 16209192.00 7134944.00 3408261.00 F 39955.00 22110.00 25824.00 16770230.00 6940105.00 3421999.00 G 17692.00 18797.00 20930.00 18223266.00 7541395.00 3515046.00 H 20358.00 51001.00 18187.00 15091851.00 6342759.00 3206333.00 Load time: 10/01/2010 09:53:47 AM Read time: 10/01/2010 09:53:47 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Fluorescence Intensity Method Plate ID: #1067 Comment: new comment Microplate format: Nunc-Immuno Flat Bottom Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f0 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42193352 cps Min counts: 2685 counts Well list: r0(a1:h6) = (f0(a1:h6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4219335200.00 3059580600.00 4148870400.00 1539663800.00 677688800.00 347092100.00 B 617748000.00 360993300.00 523461800.00 2483352400.00 1112669900.00 564385200.00 C 111744700.00 51268300.00 84463200.00 2804748400.00 1234523400.00 594473100.00 D 22387000.00 12150500.00 18320900.00 2923967200.00 1175929800.00 662407200.00 E 3409000.00 10150800.00 5837100.00 2512304800.00 1040850500.00 519797600.00 F 5713400.00 3370900.00 3951700.00 2552378400.00 1161977900.00 547994800.00 G 2685300.00 3028100.00 2923300.00 2816902400.00 1312786100.00 565935000.00 H 2837600.00 11293500.00 2751900.00 2409186800.00 1062520400.00 492821900.00 Load time: 10/01/2010 10:00:32 AM Read time: 10/01/2010 10:00:32 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Fluorescence Intensity Method Plate ID: #1069 Comment: new comment Microplate format: Nunc-Immuno Flat Bottom Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f0 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 43491896 cps Min counts: 2504 counts Well list: r0(a1:h6) = (f0(a1:h6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4349189600.00 3068909000.00 4294360800.00 1574929900.00 678313500.00 346813600.00 B 606612700.00 361059400.00 530355400.00 2496385800.00 1093140700.00 560808500.00 C 112001800.00 50258900.00 85072600.00 2752445800.00 1236225400.00 582135700.00 D 22158500.00 12064800.00 17778200.00 2874882800.00 1172025200.00 658065300.00 E 3075700.00 9969800.00 5494300.00 2570291600.00 1044823800.00 523031800.00 F 5437200.00 3132800.00 3475600.00 2667957200.00 1152782500.00 551238000.00 G 2504300.00 2685300.00 2694800.00 2846661400.00 1313781100.00 584434200.00 H 2561500.00 11388700.00 2618600.00 2403841600.00 1100170800.00 493436000.00 Load time: 10/01/2010 10:02:02 AM Read time: 10/01/2010 10:02:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: DNATech Fluorescence Int Plate ID: #1070 Comment: Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42427424 cps Min counts: 2885 counts Well list: r0(a1:h6) = (f1(a1:h6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4242742400.00 2963343800.00 3903378800.00 1335223000.00 608876000.00 308615600.00 B 543608900.00 348898400.00 564417800.00 2202780600.00 968875400.00 552645800.00 C 109659300.00 46411900.00 71427100.00 2443562400.00 1114386600.00 518190200.00 D 21968000.00 11141100.00 16492700.00 2683098600.00 1004831400.00 688764600.00 E 3370900.00 16654500.00 6379900.00 2441077200.00 946911800.00 465409500.00 F 5751400.00 3799400.00 4094600.00 2604141800.00 1204361000.00 514612800.00 G 2999500.00 3523200.00 3304200.00 2680148600.00 1425359300.00 553045500.00 H 2885200.00 15045300.00 3180400.00 2453785400.00 1236715500.00 457830900.00 Load time: 10/01/2010 10:03:10 AM Read time: 10/01/2010 10:03:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1071 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28060296 cps Min counts: 7332 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27269474.00 19840944.00 28060296.00 10178189.00 4476845.00 2417510.00 B 3974888.00 2278085.00 3157420.00 16001384.00 6949875.00 3427632.00 C 693055.00 329454.00 591928.00 18893168.00 8149974.00 3933758.00 D 140511.00 79473.00 120076.00 18110810.00 7821809.00 3945477.00 E 18739.00 44088.00 32414.00 16066340.00 6950333.00 3563188.00 F 32699.00 18511.00 20625.00 16266280.00 6874383.00 3503306.00 G 14854.00 16264.00 15578.00 17975178.00 7546363.00 3735731.00 H 16283.00 58543.00 14664.00 15031826.00 6339321.00 3206058.00