Load time: 9/8/2008 10:04 Read time: 9/8/2008 10:04 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Farnham Plate ID: #1634 Comment: Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f8 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74397160 cps Min counts: 5823 counts Well list: "r0(a1:h1,a2:h2,a3:h3) = (f8(a1:h1,a2:h2,a3:h3))" Data: RAW DATA Units: rfu Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.90E+08 6.57E+06 7.00E+09 B 7.42E+07 9.20E+06 7.43E+09 C 1.52E+08 1.22E+07 1.12E+09 D 5.55E+07 1.88E+07 1.35E+09 E 7.51E+08 1.05E+08 9.18E+08 F 2.79E+07 8.39E+08 3.46E+09 G 1.21E+08 5.82E+06 5.12E+09 H 7.38E+07 8.80E+07 7.44E+09 Load time: 9/8/2008 12:59 Read time: 9/8/2008 12:59 Kinetic read cycle: 1 of 10 Read start delay time: 0 s Time delay between kinetic reads: 0 s Barcode: Method ID: Luc Plate ID: #1635 Comment: Microplate format: Corning 96 Flat Opaque PS Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: l0 Light sensor: HC-124 Excitation side: Top Emission side: Top Lamp: None Readings per well: 10 Time between readings: 100 ms Integration time: 1600000 us Attenuator mode: o Motion settling time: 0 ms Luminescence height: 2 mm Numeric Luminescence aperture: 96 Max cps: 565 cps Min counts: 663 counts Dark Count reading: 709 counts Well list: "r0(a2:e6,b1:e1,a1) = counts(l0(a2:e6,b1:e1,a1))" Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.14E+03 6.26E+03 1.01E+03 9.22E+02 9.63E+02 9.02E+02 B 5.37E+03 7.76E+02 7.54E+02 7.71E+02 7.87E+02 8.02E+02 C 9.04E+03 7.18E+02 7.54E+02 7.43E+02 7.64E+02 7.46E+02 D 5.48E+03 6.92E+02 7.23E+02 6.63E+02 7.75E+02 7.86E+02 E 7.05E+02 7.19E+02 7.18E+02 7.17E+02 7.16E+02 7.21E+02 F G H Well list: "r1(a2:e6,b1:e1) = counts(l0(a2:e6,b1:e1) - avg(l0(a1)))" Data: BACKGROUND SUBTRACTED DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 5.12E+03 -1.26E+02 -2.18E+02 -1.77E+02 -2.38E+02 B 4.23E+03 -3.64E+02 -3.86E+02 -3.69E+02 -3.53E+02 -3.38E+02 C 7.90E+03 -4.22E+02 -3.86E+02 -3.97E+02 -3.76E+02 -3.94E+02 D 4.34E+03 -4.48E+02 -4.17E+02 -4.77E+02 -3.65E+02 -3.54E+02 E -4.35E+02 -4.21E+02 -4.22E+02 -4.23E+02 -4.24E+02 -4.19E+02 F G H Load time: 9/8/2008 12:59 Read time: 9/8/2008 13:08 Kinetic read cycle: 2 of 10 Read start delay time: 0 s Time delay between kinetic reads: 0 s Barcode: Method ID: Luc Plate ID: #1635 Comment: Microplate format: Corning 96 Flat Opaque PS Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: l0 Light sensor: HC-124 Excitation side: Top Emission side: Top Lamp: None Readings per well: 10 Time between readings: 100 ms Integration time: 1600000 us Attenuator mode: o Motion settling time: 0 ms Luminescence height: 2 mm Numeric Luminescence aperture: 96 Max cps: 554 cps Min counts: 668 counts Dark Count reading: 676 counts Well list: "r0(a2:e6,b1:e1,a1) = counts(l0(a2:e6,b1:e1,a1))" Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 6.83E+02 6.24E+03 7.55E+02 7.16E+02 6.96E+02 6.77E+02 B 5.18E+03 7.43E+02 7.12E+02 6.78E+02 7.31E+02 7.58E+02 C 8.88E+03 7.17E+02 6.77E+02 6.93E+02 7.27E+02 6.68E+02 D 4.76E+03 6.75E+02 7.85E+02 6.88E+02 6.77E+02 7.33E+02 E 7.00E+02 7.22E+02 7.28E+02 7.31E+02 6.93E+02 6.83E+02 F G H Well list: "r1(a2:e6,b1:e1) = counts(l0(a2:e6,b1:e1) - avg(l0(a1)))" Data: BACKGROUND SUBTRACTED DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 5.55E+03 7.20E+01 3.30E+01 1.30E+01 -6.00E+00 B 4.50E+03 6.00E+01 2.90E+01 -5.00E+00 4.80E+01 7.50E+01 C 8.19E+03 3.40E+01 -6.00E+00 1.00E+01 4.40E+01 -1.50E+01 D 4.07E+03 -8.00E+00 1.02E+02 5.00E+00 -6.00E+00 5.00E+01 E 1.70E+01 3.90E+01 4.50E+01 4.80E+01 1.00E+01 0.00E+00 F G H