Load time: 02/22/2008 03:23:38 PM Read time: 02/22/2008 03:23:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: DavesMethod Plate ID: #1400 Comment: SSA Assay Microplate format: Corning 96 Flat Opaque PS Shake time: 10 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: l1 Light sensor: HC-124 Excitation side: Top Emission side: Top Lamp: None Readings per well: 10 Time between readings: 100 ms Integration time: 1600000 us Attenuator mode: o Motion settling time: 0 ms Luminescence height: 2 mm Numeric Luminescence aperture: 96 Max cps: 92726 cps Min counts: 537 counts Dark Count reading: 425 counts Well list: r0(d1:e7,d8:e8) = counts(l1(d1:e7,d8:e8)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 2.662200000e+005 3.331000000e+003 4.096700000e+004 7.140000000e+002 9.465000000e+003 3.249560000e+005 1.003000000e+003 8.020000000e+002 E 1.483618000e+006 2.690400000e+004 3.672500000e+004 9.800000000e+002 1.022900000e+004 8.095640000e+005 1.172000000e+003 5.370000000e+002 F G H Well list: r1(d1:e7) = counts(l1(d1:e7) - avg(l1(d8:e8))) Data: BACKGROUND SUBTRACTED DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 2.655505000e+005 2.661500000e+003 4.029750000e+004 4.450000000e+001 8.795500000e+003 3.242865000e+005 3.335000000e+002 E 1.482948500e+006 2.623450000e+004 3.605550000e+004 3.105000000e+002 9.559500000e+003 8.088945000e+005 5.025000000e+002 F G H Load time: 03/03/2008 11:37:13 AM Read time: 03/03/2008 11:37:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: DavesMethod Plate ID: #1402 Comment: H99 MIC Microplate format: Corning 96 Flat Opaque PS Shake time: 10 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: l1 Light sensor: HC-124 Excitation side: Top Emission side: Top Lamp: None Readings per well: 10 Time between readings: 100 ms Integration time: 1600000 us Attenuator mode: o Motion settling time: 0 ms Luminescence height: 2 mm Numeric Luminescence aperture: 96 Max cps: 59 cps Min counts: 726 counts Dark Count reading: 552 counts Well list: r0(d1:e7,d8:e8) = counts(l1(d1:e7,d8:e8)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 8.130000000e+002 8.360000000e+002 7.910000000e+002 8.770000000e+002 8.220000000e+002 9.070000000e+002 8.930000000e+002 8.690000000e+002 E 7.360000000e+002 7.260000000e+002 8.810000000e+002 9.540000000e+002 7.660000000e+002 8.480000000e+002 8.330000000e+002 8.080000000e+002 F G H Well list: r1(d1:e7) = counts(l1(d1:e7) - avg(l1(d8:e8))) Data: BACKGROUND SUBTRACTED DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D -2.550000000e+001 -2.500000000e+000 -4.750000000e+001 3.850000000e+001 -1.650000000e+001 6.850000000e+001 5.450000000e+001 E -1.025000000e+002 -1.125000000e+002 4.250000000e+001 1.155000000e+002 -7.250000000e+001 9.500000000e+000 -5.500000000e+000 F G H Load time: 03/03/2008 11:49:16 AM Read time: 03/03/2008 11:49:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1403 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 2 Photodiode resistor: 4 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Well list: r0(a1) = counts(a0(a1)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.638300000e+004 B C D E F G H Load time: 03/03/2008 11:50:59 AM Read time: 03/03/2008 11:51:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1404 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 2 Photodiode resistor: 4 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Well list: r0(a1) = counts(a0(a1)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.638300000e+004 B C D E F G H Load time: 03/03/2008 11:52:30 AM Read time: 03/03/2008 11:52:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1405 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 2 Photodiode resistor: 4 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Well list: r0(a1) = counts(a0(a1)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.638300000e+004 B C D E F G H Load time: 03/03/2008 11:53:31 AM Read time: 03/03/2008 11:53:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1406 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 2 Photodiode resistor: 4 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Well list: r0(a1) = counts(a0(a1)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.638300000e+004 B C D E F G H Load time: 03/03/2008 11:55:19 AM Read time: 03/03/2008 11:55:22 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1407 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 2 Europium 360 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 2 Photodiode resistor: 4 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Well list: r0(a1) = counts(a0(a1)) Data: RAW DATA Units: counts Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A 1.638300000e+004 B C D E F G H Load time: 03/03/2008 11:56:27 AM Read time: 03/03/2008 11:56:29 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1409 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 40000 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 2 Europium 360 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Dark Count reading: 215 counts Well list: r0(g4) = cps(a0(g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G 16383 H Well list: r2(g4) = 0 - log10((a0(g4)-216)/(1 - 216)) Data: 0 (Abs factor) - Log10( (Sample 1 - Dark counts) / (Bright counts - Dark counts)) Units: O.D. Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G -1.#IND00000e+000 H Load time: 03/03/2008 12:01:34 PM Read time: 03/03/2008 12:01:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1412 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 21500 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 2 Europium 360 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 7 Photodiode gain: x2 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Dark Count reading: 929 counts Well list: r0(g4) = cps(a0(g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G 16383 H Well list: r2(g4) = 0 - log10((a0(g4)-930)/(16383 - 930)) Data: 0 (Abs factor) - Log10( (Sample 1 - Dark counts) / (Bright counts - Dark counts)) Units: O.D. Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G 0.000000000e+000 H Load time: 03/03/2008 12:03:16 PM Read time: 03/03/2008 12:03:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1413 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 21500 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 2 Europium 360 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 7 Photodiode gain: x2 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 16383 counts Dark Count reading: 930 counts Well list: r0(a1:f12,g4) = cps(a0(a1:f12,g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 B 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 C 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 D 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 E 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 F 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 G 16383 H Well list: r1(a1:f12) = Counts(a0(a1:f12)-928) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 B 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 C 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 D 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 E 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 F 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 15455 G H Well list: r2(a1:f12) = log10((avg(a0(g4))-928)/(a0(a1:f12) - 928)),r2(g4) = log10((avg(a0(g4))-928)/(a0(g4) - 928)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 B 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 C 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 D 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 E 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 F 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 G 0.000 H Load time: 03/03/2008 12:06:04 PM Read time: 03/03/2008 12:06:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1415 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 5601 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 9026 counts Dark Count reading: 30 counts Well list: r0(a1:f12,g4) = cps(a0(a1:f12,g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16383 16383 16383 16383 16383 15478 14895 9026 15219 13628 14152 14202 B 16383 16383 16383 15847 16383 15031 15065 14701 16383 16383 9268 9907 C 16383 16383 15780 16113 15642 16296 14900 15011 16383 16383 9376 9888 D 16383 16383 16383 16383 16383 15567 14704 15239 13288 14992 14657 14857 E 16383 16383 16251 16383 15677 15388 15323 14554 16383 16383 9959 9191 F 16339 16383 16383 15869 15817 14738 15644 14079 16383 16383 9397 10166 G 14545 H Well list: r1(a1:f12) = Counts(a0(a1:f12)-31) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16352 16352 16352 16352 16352 15447 14864 8995 15188 13597 14121 14171 B 16352 16352 16352 15816 16352 15000 15034 14670 16352 16352 9237 9876 C 16352 16352 15749 16082 15611 16265 14869 14980 16352 16352 9345 9857 D 16352 16352 16352 16352 16352 15536 14673 15208 13257 14961 14626 14826 E 16352 16352 16220 16352 15646 15357 15292 14523 16352 16352 9928 9160 F 16308 16352 16352 15838 15786 14707 15613 14048 16352 16352 9366 10135 G H Well list: r2(a1:f12) = log10((avg(a0(g4))-31)/(a0(a1:f12) - 31)),r2(g4) = log10((avg(a0(g4))-31)/(a0(g4) - 31)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A -0.052 -0.052 -0.052 -0.052 -0.052 -0.027 -0.010 0.208 -0.020 0.028 0.012 0.010 B -0.052 -0.052 -0.052 -0.037 -0.052 -0.014 -0.015 -0.005 -0.052 -0.052 0.196 0.167 C -0.052 -0.052 -0.035 -0.045 -0.032 -0.049 -0.010 -0.014 -0.052 -0.052 0.191 0.168 D -0.052 -0.052 -0.052 -0.052 -0.052 -0.030 -0.005 -0.020 0.039 -0.013 -0.003 -0.009 E -0.052 -0.052 -0.048 -0.052 -0.033 -0.025 -0.023 -0.000 -0.052 -0.052 0.165 0.200 F -0.051 -0.052 -0.052 -0.038 -0.036 -0.006 -0.032 0.014 -0.052 -0.052 0.190 0.156 G 0.000 H Load time: 03/03/2008 12:10:01 PM Read time: 03/03/2008 12:10:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1416 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 5601 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 9412 counts Dark Count reading: 31 counts Well list: r0(g4,b1:f12,a1:a12) = cps(a0(g4,b1:f12,a1:a12)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16383 16383 16383 16383 16341 15786 13983 9471 14667 14065 14191 13894 B 16383 16314 16383 15595 16217 14585 15254 14219 16383 16383 9825 10048 C 16383 15910 16383 15198 16226 15640 15468 14159 16383 16383 9593 9896 D 16383 16383 16383 16383 16383 15760 14413 14575 13891 14168 14703 15290 E 16383 16383 16258 16155 15655 15217 15814 14256 16383 16383 9695 9412 F 16383 16152 16383 15267 16111 14991 15414 14183 16383 16383 9652 9652 G 14603 H Well list: r2(g4,b1:f12,a1:a12) = 0 - log10((a0(g4,b1:f12,a1:a12)-31)/(14210 - 31)) Data: 0 (Abs factor) - Log10( (Sample 1 - Dark counts) / (Bright counts - Dark counts)) Units: O.D. Display format: %.9e 1 2 3 4 5 6 7 8 9 10 11 12 A -6.192527594e-002 -6.192527594e-002 -6.192527594e-002 -6.192527594e-002 -6.080835853e-002 -4.577280500e-002 7.009134923e-003 1.766736082e-001 -1.377679830e-002 4.464130319e-003 5.823491579e-004 9.788379273e-003 B -6.192527594e-002 -6.008882036e-002 -6.192527594e-002 -4.047561962e-002 -5.749393354e-002 -1.133676807e-002 -3.085464487e-002 -2.755772883e-004 -6.192527594e-002 -6.192527594e-002 1.606855028e-001 1.509079287e-001 C -6.192527594e-002 -4.917754620e-002 -6.192527594e-002 -2.925408425e-002 -5.773534983e-002 -4.172947810e-002 -3.691730165e-002 1.564916278e-003 -6.192527594e-002 -6.192527594e-002 1.710968632e-001 1.575485129e-001 D -6.192527594e-002 -6.192527594e-002 -6.192527594e-002 -6.192527594e-002 -6.192527594e-002 -4.505550992e-002 -6.173681906e-003 -1.103826337e-002 9.882372236e-003 1.288344513e-003 -1.484371581e-002 -3.188047051e-002 E -6.192527594e-002 -6.192527594e-002 -5.859263365e-002 -5.582718697e-002 -4.214662777e-002 -2.979779303e-002 -4.654395401e-002 -1.406672555e-003 -6.192527594e-002 -6.192527594e-002 1.664886812e-001 1.793964665e-001 F -6.192527594e-002 -5.574637547e-002 -6.192527594e-002 -3.122536148e-002 -5.464044190e-002 -2.328599102e-002 -3.539543753e-002 8.277826100e-004 -6.192527594e-002 -6.192527594e-002 1.684253879e-001 1.684253879e-001 G -1.187356006e-002 H Load time: 03/03/2008 12:12:00 PM Read time: 03/03/2008 12:12:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1418 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 5601 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 8735 counts Dark Count reading: 31 counts Well list: r0(g4,b1:f12,a1:a12,g5) = cps(a0(g4,b1:f12,a1:a12,g5)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16383 16383 16383 16383 16383 14995 14590 8735 14982 13589 13725 13962 B 16383 16383 16003 15830 16262 14834 14970 14272 16383 16347 9674 9836 C 16383 15592 16383 15105 16196 14969 15627 14162 16383 16383 9221 10207 D 16383 16383 16383 16383 16383 15376 14737 14454 14100 14049 15250 14523 E 16383 16350 16109 16383 15820 15631 15178 14367 16383 16383 9814 9274 F 16180 16057 16383 15435 15142 15433 14812 13981 16383 16383 9350 9694 G 14767 16383 H Well list: r1(g4,b1:f12,a1:a12) = Counts(a0(g4,b1:f12,a1:a12)-31) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16352 16352 16352 16352 16352 14964 14559 8704 14951 13558 13694 13931 B 16352 16352 15972 15799 16231 14803 14939 14241 16352 16316 9643 9805 C 16352 15561 16352 15074 16165 14938 15596 14131 16352 16352 9190 10176 D 16352 16352 16352 16352 16352 15345 14706 14423 14069 14018 15219 14492 E 16352 16319 16078 16352 15789 15600 15147 14336 16352 16352 9783 9243 F 16149 16026 16352 15404 15111 15402 14781 13950 16352 16352 9319 9663 G 14736 H Well list: r2(g4,b1:f12,a1:a12) = log10((avg(a0(g5))-31)/(a0(g4,b1:f12,a1:a12) - 31)),r2(g5) = log10((avg(a0(g5))-31)/(a0(g5) - 31)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A 0.000 0.000 0.000 0.000 0.000 0.039 0.050 0.274 0.039 0.081 0.077 0.070 B 0.000 0.000 0.010 0.015 0.003 0.043 0.039 0.060 0.000 0.001 0.229 0.222 C 0.000 0.022 0.000 0.035 0.005 0.039 0.021 0.063 0.000 0.000 0.250 0.206 D 0.000 0.000 0.000 0.000 0.000 0.028 0.046 0.055 0.065 0.067 0.031 0.052 E 0.000 0.001 0.007 0.000 0.015 0.020 0.033 0.057 0.000 0.000 0.223 0.248 F 0.005 0.009 0.000 0.026 0.034 0.026 0.044 0.069 0.000 0.000 0.244 0.228 G 0.045 0.000 H Load time: 03/03/2008 12:18:47 PM Read time: 03/03/2008 12:18:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1420 Comment: new comment Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 4734 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 7540 counts Dark Count reading: 25 counts Well list: r0(g4,b1:f12,a1:a12,g5) = cps(a0(g4,b1:f12,a1:a12,g5)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 14844 16383 15677 14792 14357 12891 11889 7964 12227 11677 11954 11946 B 16383 13517 14424 13226 13119 12934 12056 12699 16383 14397 8188 8283 C 14823 13432 13947 12798 13370 12827 12762 12674 16383 16383 7540 8495 D 15659 16383 15092 15982 14183 12874 12625 12740 12200 11926 12931 12524 E 16383 13798 13404 13907 12914 12862 13487 12136 16383 16070 8316 8348 F 14316 13623 13787 13137 13173 12313 12936 11820 15893 16383 8040 8038 G 12374 14325 H Well list: r1(g4,b1:f12,a1:a12) = Counts(a0(g4,b1:f12,a1:a12)-25) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 14819 16358 15652 14767 14332 12866 11864 7939 12202 11652 11929 11921 B 16358 13492 14399 13201 13094 12909 12031 12674 16358 14372 8163 8258 C 14798 13407 13922 12773 13345 12802 12737 12649 16358 16358 7515 8470 D 15634 16358 15067 15957 14158 12849 12600 12715 12175 11901 12906 12499 E 16358 13773 13379 13882 12889 12837 13462 12111 16358 16045 8291 8323 F 14291 13598 13762 13112 13148 12288 12911 11795 15868 16358 8015 8013 G 12349 H Well list: r2(g4,b1:f12,a1:a12) = log10((avg(a0(g5))-25)/(a0(g4,b1:f12,a1:a12) - 25)),r2(g5) = log10((avg(a0(g5))-25)/(a0(g5) - 25)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A -0.015 -0.058 -0.039 -0.014 -0.001 0.046 0.081 0.256 0.069 0.089 0.079 0.079 B -0.058 0.025 -0.003 0.035 0.038 0.044 0.075 0.052 -0.058 -0.002 0.243 0.238 C -0.015 0.028 0.012 0.049 0.030 0.048 0.050 0.053 -0.058 -0.058 0.279 0.227 D -0.039 -0.058 -0.023 -0.048 0.004 0.046 0.055 0.051 0.070 0.080 0.045 0.058 E -0.058 0.016 0.029 0.013 0.045 0.047 0.026 0.072 -0.058 -0.050 0.237 0.235 F 0.000 0.022 0.017 0.038 0.036 0.066 0.044 0.084 -0.045 -0.058 0.251 0.252 G 0.064 0.000 H Load time: 03/03/2008 12:26:08 PM Read time: 03/03/2008 12:26:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1421 Comment: H99 MIC Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 4734 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 7050 counts Dark Count reading: 24 counts Well list: r0(a1:f12,g4) = cps(a0(a1:f12,g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 15000 14861 14450 16383 15068 15398 13896 12472 12189 11928 12365 12980 B 16383 16273 13626 13280 12106 11692 10930 12215 16383 16303 8057 7741 C 14987 14301 13253 10240 11510 12113 11133 11148 16250 16383 7050 8148 D 15069 16383 14847 16383 16383 16325 14444 13850 12193 12498 13182 12943 E 16383 16383 13649 11941 12967 11374 10137 9651 16383 16254 8393 7792 F 14904 13728 12486 11604 12244 10795 9573 10564 16383 15963 7621 8118 G 12183 H Well list: r1(a1:f12) = Counts(a0(a1:f12)-26) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 14974 14835 14424 16357 15042 15372 13870 12446 12163 11902 12339 12954 B 16357 16247 13600 13254 12080 11666 10904 12189 16357 16277 8031 7715 C 14961 14275 13227 10214 11484 12087 11107 11122 16224 16357 7024 8122 D 15043 16357 14821 16357 16357 16299 14418 13824 12167 12472 13156 12917 E 16357 16357 13623 11915 12941 11348 10111 9625 16357 16228 8367 7766 F 14878 13702 12460 11578 12218 10769 9547 10538 16357 15937 7595 8092 G H Well list: r2(a1:f12) = log10((avg(a0(g4))-26)/(a0(a1:f12) - 26)),r2(g4) = log10((avg(a0(g4))-26)/(a0(g4) - 26)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A -0.091 -0.086 -0.074 -0.129 -0.092 -0.102 -0.057 -0.010 -0.000 0.009 -0.006 -0.028 B -0.129 -0.126 -0.049 -0.038 0.003 0.018 0.047 -0.001 -0.129 -0.127 0.180 0.197 C -0.090 -0.070 -0.037 0.076 0.025 0.003 0.039 0.039 -0.125 -0.129 0.238 0.175 D -0.093 -0.129 -0.086 -0.129 -0.129 -0.127 -0.074 -0.056 -0.000 -0.011 -0.034 -0.026 E -0.129 -0.129 -0.049 0.009 -0.027 0.030 0.080 0.101 -0.129 -0.125 0.162 0.195 F -0.088 -0.052 -0.011 0.021 -0.002 0.053 0.105 0.062 -0.129 -0.118 0.204 0.177 G 0.000 H Load time: 03/03/2008 12:30:18 PM Read time: 03/03/2008 12:30:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1424 Comment: H99 MIC Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 6468 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16383 cps Min counts: 9988 counts Dark Count reading: 35 counts Well list: r0(a1:f12,g4) = cps(a0(a1:f12,g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 B 16383 16383 16383 16383 16383 16383 14178 16383 16383 16383 10224 11366 C 16383 16383 16383 13122 16383 16383 15636 16383 16383 16383 9988 10759 D 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 16383 E 16383 16383 16383 16383 16383 16383 13809 13273 16383 16383 10859 10806 F 16383 16383 16383 16383 16383 15077 14470 14927 16383 16383 10120 11481 G 16383 H Well list: r1(a1:f12) = Counts(a0(a1:f12)-36) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 B 16347 16347 16347 16347 16347 16347 14142 16347 16347 16347 10188 11330 C 16347 16347 16347 13086 16347 16347 15600 16347 16347 16347 9952 10723 D 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 16347 E 16347 16347 16347 16347 16347 16347 13773 13237 16347 16347 10823 10770 F 16347 16347 16347 16347 16347 15041 14434 14891 16347 16347 10084 11445 G H Well list: r2(a1:f12) = log10((avg(a0(g4))-36)/(a0(a1:f12) - 36)),r2(g4) = log10((avg(a0(g4))-36)/(a0(g4) - 36)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 B 0.000 0.000 0.000 0.000 0.000 0.000 0.063 0.000 0.000 0.000 0.205 0.159 C 0.000 0.000 0.000 0.097 0.000 0.000 0.020 0.000 0.000 0.000 0.216 0.183 D 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 E 0.000 0.000 0.000 0.000 0.000 0.000 0.074 0.092 0.000 0.000 0.179 0.181 F 0.000 0.000 0.000 0.000 0.000 0.036 0.054 0.041 0.000 0.000 0.210 0.155 G 0.000 H Load time: 03/03/2008 12:34:48 PM Read time: 03/03/2008 12:35:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Default Absorbance Method Plate ID: #1428 Comment: H99 MIC Microplate format: Corning 96 Flat Clear PS Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a0 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 1 Time between readings: Integration time: 5890 us Delay after flash: Motion settling time: 0 ms Z Height: 5.33 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 16213 cps Min counts: 9855 counts Dark Count reading: 31 counts Well list: r0(a1:f12,g4) = cps(a0(a1:f12,g4)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 12837 15927 15368 15319 16213 15056 15691 15565 15097 15792 15649 15721 B 11408 10578 14332 10287 15077 15935 15782 15688 15226 14641 15494 14487 C 11638 12594 11890 12543 15186 14618 15544 15370 15182 14906 14970 13885 D 12877 10592 11969 13282 11054 14659 15642 13907 15166 13851 14728 14779 E 11548 10677 11219 11736 10767 13039 15283 12006 13154 13454 13442 12297 F 9855 11115 11285 10176 12099 12585 12496 11440 11074 13788 12938 14820 G 11658 H Well list: r1(a1:f12) = Counts(a0(a1:f12)-31) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 12806 15896 15337 15288 16182 15025 15660 15534 15066 15761 15618 15690 B 11377 10547 14301 10256 15046 15904 15751 15657 15195 14610 15463 14456 C 11607 12563 11859 12512 15155 14587 15513 15339 15151 14875 14939 13854 D 12846 10561 11938 13251 11023 14628 15611 13876 15135 13820 14697 14748 E 11517 10646 11188 11705 10736 13008 15252 11975 13123 13423 13411 12266 F 9824 11084 11254 10145 12068 12554 12465 11409 11043 13757 12907 14789 G H Well list: r2(a1:f12) = log10((avg(a0(g4))-31)/(a0(a1:f12) - 31)),r2(g4) = log10((avg(a0(g4))-31)/(a0(g4) - 31)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A -0.042 -0.136 -0.120 -0.119 -0.144 -0.111 -0.129 -0.126 -0.113 -0.132 -0.128 -0.130 B 0.009 0.042 -0.090 0.054 -0.112 -0.136 -0.132 -0.129 -0.116 -0.099 -0.124 -0.095 C 0.001 -0.034 -0.009 -0.032 -0.115 -0.098 -0.125 -0.120 -0.115 -0.107 -0.109 -0.076 D -0.043 0.042 -0.011 -0.057 0.023 -0.100 -0.128 -0.077 -0.115 -0.075 -0.102 -0.103 E 0.004 0.038 0.017 -0.003 0.035 -0.049 -0.118 -0.013 -0.053 -0.062 -0.062 -0.023 F 0.073 0.021 0.014 0.059 -0.016 -0.033 -0.030 0.008 0.022 -0.073 -0.045 -0.104 G 0.000 H