Load time: 07/06/2012 02:34:30 PM Read time: 07/06/2012 02:34:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2746 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74501176 cps Min counts: 7199 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72036856.00 74501176.00 62545372.00 18064.00 B 46929984.00 49299220.00 49535224.00 14398.00 C 27192848.00 32383278.00 31792156.00 18216.00 D 17514120.00 18331202.00 17553126.00 15211.00 E 8436468.00 9109677.00 9635704.00 15665.00 F 5310519.00 5819608.00 5756348.00 16704.00 G 3144788.00 3493904.00 3654065.00 18480.00 H 1765059.00 2010733.00 2079667.00 19708.00