Load time: 07/06/2012 02:37:27 PM Read time: 07/06/2012 02:37:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2747 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11021394 cps Min counts: 26237 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 532139.00 153589.00 1474554.00 178418.00 1233003.00 125452.00 832873.00 464206.00 233709.00 96144.00 1032588.00 1684567.00 B 637467.00 539546.00 1801033.00 2180420.00 628377.00 513318.00 1028979.00 540037.00 170180.00 580097.00 1200010.00 902450.00 C 505230.00 1549852.00 413621.00 857816.00 1410118.00 583914.00 6761528.00 254590.00 3929569.00 986727.00 8170539.00 811199.00 D 52475.00 2068865.00 632365.00 3044666.00 2240873.00 424203.00 1597088.00 3253696.00 1219738.00 1299858.00 11021394.00 1624788.00 E 79591.00 1271400.00 699428.00 114530.00 1815205.00 3969999.00 4424295.00 968492.00 2216066.00 766358.00 231725.00 120104.00 F 1133041.00 1081794.00 1279242.00 1760665.00 5042459.00 1168491.00 6003458.00 5053125.00 3282675.00 493042.00 1463084.00 968965.00 G 1834989.00 5915110.00 1162274.00 1823688.00 2520305.00 1159534.00 2463286.00 800277.00 1448629.00 615131.00 1048896.00 1650708.00 H 2677199.00 1755942.00 1747005.00 2370615.00 7355052.00 1628153.00 1068491.00 134617.00 610728.00 3691287.00 211449.00 1121911.00