Load time: 04/23/2013 11:50:01 AM Read time: 04/23/2013 11:50:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3021 Comment: std 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30979392 cps Min counts: 1188 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23232750.00 30979392.00 28837746.00 168034.00 548403.00 1052186.00 423839.00 979050.00 502839.00 1090111.00 707894.00 440397.00 B 13346742.00 16091006.00 13995887.00 176125.00 537521.00 243621.00 305366.00 592326.00 192249.00 312099.00 662896.00 463971.00 C 4978159.00 9077191.00 7688192.00 178897.00 221047.00 256464.00 179670.00 179161.00 437078.00 2696.00 2527.00 2376.00 D 4240345.00 5183827.00 4779503.00 179425.00 657408.00 650166.00 678549.00 199189.00 213032.00 1925163.00 2176054.00 1471046.00 E 3007043.00 3404652.00 2925295.00 183366.00 949611.00 663443.00 825914.00 1221597.00 190816.00 1007999.00 1611016.00 160094.00 F 1647956.00 2067633.00 1736629.00 182159.00 172164.00 159830.00 169052.00 168091.00 163904.00 170429.00 167072.00 165809.00 G 1048697.00 1342974.00 900087.00 182725.00 239359.00 1165963.00 273852.00 982218.00 234644.00 1049791.00 466215.00 297690.00 H 768281.00 788724.00 703707.00 172579.00 264875.00 1015938.00 167864.00 2489.00 2413.00 2508.00 2451.00 2545.00