Load time: 01/31/2012 11:26:28 AM Read time: 01/31/2012 11:26:28 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2181 Comment: Wheat Plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10655551 cps Min counts: 292039 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1728269.00 1396876.00 972690.00 1519006.00 910844.00 5281129.00 1705671.00 4146522.00 1836870.00 1660879.00 705238.00 2402049.00 B 1700353.00 3087126.00 1120559.00 916057.00 1992606.00 1706536.00 1593654.00 4939932.00 1892330.00 2073871.00 1586112.00 4317701.00 C 1699260.00 1619429.00 1281175.00 2205837.00 2128421.00 2610674.00 1640344.00 10655551.00 4222540.00 2611751.00 3490596.00 3470557.00 D 2196404.00 1927131.00 1657722.00 1462221.00 2514672.00 3700131.00 2675752.00 5609855.00 3042318.00 3539434.00 2589317.00 2906854.00 E 3798788.00 1927264.00 1312640.00 1143768.00 2084031.00 3834931.00 1763353.00 3124320.00 1260781.00 1849639.00 2453962.00 3325556.00 F 3127890.00 3109869.00 1601773.00 1051181.00 6567592.00 4091818.00 2435526.00 5154810.00 2510067.00 2665070.00 3020830.00 8883624.00 G 1704057.00 2075402.00 1343515.00 1146165.00 3648023.00 1778431.00 2534959.00 4319915.00 2050996.00 3663048.00 2348188.00 1284637.00 H 2134429.00 1523648.00 1027858.00 1846460.00 1820037.00 3033807.00 1549843.00 2599484.00 1461517.00 2202577.00 584078.00 1796499.00