Load time: 05/04/2012 01:47:27 PM Read time: 05/04/2012 01:47:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2543 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10132011 cps Min counts: 81624 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 350862.00 491600.00 1068319.00 385423.00 289294.00 1584196.00 163249.00 1008470.00 1608938.00 199208.00 184817.00 251805.00 B 1066770.00 760346.00 1379955.00 1110435.00 1418502.00 1946904.00 5285381.00 1319785.00 1201200.00 1522865.00 2975807.00 451165.00 C 1017705.00 1460296.00 2126438.00 1702452.00 2012015.00 10132011.00 1516255.00 2322930.00 1799619.00 1585685.00 2914907.00 2145011.00 D 1449267.00 1466812.00 1309624.00 1519258.00 1890789.00 2302820.00 1018064.00 1696858.00 498965.00 2219679.00 2005565.00 2728619.00 E 1164807.00 1191342.00 1023710.00 2122038.00 1390003.00 1857297.00 2984819.00 1657452.00 1954436.00 1572349.00 3881717.00 2440991.00 F 519173.00 2440284.00 1188717.00 1056062.00 1961403.00 1594644.00 1158235.00 1160822.00 2223086.00 2181074.00 1883509.00 2129096.00 G 957591.00 2990572.00 1510703.00 446217.00 672735.00 916590.00 846409.00 936911.00 1879343.00 848865.00 870753.00 526917.00 H 292184.00 1151663.00 698174.00 787127.00 429522.00 380267.00 383761.00 391146.00 968224.00 972379.00 548956.00 364195.00 Load time: 05/04/2012 01:50:52 PM Read time: 05/04/2012 01:50:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2544 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 6380449 cps Min counts: 101483 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 202966.00 367066.00 321476.00 229501.00 230502.00 2020841.00 2262721.00 739156.00 823595.00 598438.00 362118.00 856457.00 B 581365.00 661932.00 2088408.00 3493883.00 4035594.00 4565295.00 5391754.00 880140.00 2561536.00 814455.00 1116648.00 1660877.00 C 1055987.00 1163466.00 2126676.00 4520774.00 3033576.00 1392892.00 2750351.00 2302693.00 2759555.00 1434271.00 1096780.00 2554036.00 D 1475707.00 1594439.00 4368901.00 6380449.00 4067636.00 3387337.00 2254241.00 4741530.00 1290398.00 2496665.00 4151930.00 1471099.00 E 1210077.00 1678091.00 3080353.00 2090394.00 2345847.00 3419854.00 2281802.00 2303994.00 2376608.00 973512.00 3746903.00 2157318.00 F 687390.00 1245658.00 1348548.00 2877150.00 2065291.00 1511061.00 1721078.00 1532195.00 2622491.00 1853394.00 3158959.00 1518559.00 G 623008.00 2092538.00 2794944.00 4443976.00 549882.00 2669317.00 2505558.00 2120070.00 2735305.00 1085789.00 3989484.00 1871138.00 H 397057.00 324535.00 397416.00 1943362.00 2849828.00 2999636.00 255620.00 1893780.00 2957824.00 829318.00 242627.00 358039.00