Load time: 05/04/2012 01:54:37 PM Read time: 05/04/2012 01:54:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2545 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9594105 cps Min counts: 356679 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3707649.00 4802517.00 2526470.00 1735212.00 3714312.00 3286653.00 3018238.00 4070577.00 5223475.00 2169222.00 1893146.00 2915729.00 B 1193816.00 4252622.00 4986289.00 4037894.00 2671789.00 3724723.00 2339277.00 1805167.00 3306305.00 3523352.00 2905486.00 2400371.00 C 1283958.00 4046632.00 1957892.00 4211983.00 2506709.00 3193085.00 3103561.00 2403910.00 3562855.00 964824.00 2115061.00 3456706.00 D 1720452.00 2683636.00 1968979.00 9594105.00 2898020.00 3986147.00 2876880.00 1459881.00 1542242.00 2438596.00 3017382.00 4260982.00 E 1228415.00 4406591.00 3067579.00 4306015.00 2882309.00 5896853.00 1119859.00 1218462.00 2127671.00 1465754.00 885957.00 3037178.00 F 2076839.00 3037473.00 4979641.00 3267918.00 2607273.00 5469912.00 713358.00 1491855.00 1043144.00 1518672.00 844426.00 1010471.00 G 1630595.00 3117559.00 4580281.00 3727875.00 3242351.00 6321856.00 3480190.00 746881.00 1685707.00 1241786.00 1167904.00 1877556.00 H 2536385.00 3780279.00 3628619.00 5036777.00 5207567.00 4764640.00 3746467.00 1080161.00 2252413.00 2800436.00 1439106.00 1068508.00