Load time: 05/04/2012 01:58:30 PM Read time: 05/04/2012 01:58:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2546 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3841193 cps Min counts: 175601 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1306282.00 1472232.00 1127149.00 1865616.00 1283014.00 2560682.00 1130850.00 1071832.00 1229246.00 1412798.00 827278.00 914342.00 B 1625845.00 796079.00 1180879.00 1415367.00 1811081.00 781518.00 2079271.00 2038164.00 1531477.00 3841193.00 1347812.00 1687973.00 C 2777638.00 2211459.00 1645502.00 2077524.00 1858858.00 1079197.00 1713161.00 1618797.00 2253038.00 1357217.00 1779643.00 1057044.00 D 1905144.00 1284865.00 1542148.00 2410426.00 1944234.00 2218219.00 1170379.00 811017.00 725502.00 1490797.00 2288531.00 1071530.00 E 2258513.00 2076765.00 634358.00 2891312.00 2319696.00 1949171.00 991869.00 837174.00 1221956.00 1781368.00 927940.00 1181937.00 F 1534537.00 2868348.00 1876577.00 2146570.00 2491755.00 2062554.00 1947606.00 959952.00 1634619.00 1991230.00 3058223.00 1763854.00 G 1235762.00 1884609.00 1224808.00 1850978.00 1813404.00 1706471.00 1362732.00 1901052.00 1238708.00 1739030.00 648466.00 1158159.00 H 1472440.00 1179349.00 656606.00 1176743.00 1522601.00 1349266.00 891925.00 1013248.00 1349379.00 911660.00 734510.00 351202.00