Load time: 05/04/2012 02:02:04 PM Read time: 05/04/2012 02:02:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2547 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13613456 cps Min counts: 15042 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3444115.00 5442736.00 5243366.00 1539334.00 6073064.00 1129660.00 4567273.00 3814048.00 4386605.00 5485006.00 2775485.00 5084396.00 B 2379517.00 5324134.00 8585725.00 2026933.00 8449352.00 5514448.00 4469844.00 2803483.00 4310218.00 7023003.00 4539658.00 8768731.00 C 3049643.00 9727232.00 7476901.00 2915430.00 8466868.00 1632917.00 5935889.00 5415026.00 1357576.00 12688893.00 13613456.00 2271526.00 D 5025462.00 4623162.00 6541421.00 7237227.00 8584467.00 8977208.00 5818970.00 3580801.00 1107488.00 9043863.00 7192539.00 11033444.00 E 3613421.00 4895005.00 3785690.00 2062229.00 4492089.00 7983953.00 5474331.00 4142909.00 4000205.00 4742783.00 3277893.00 8006662.00 F 1888279.00 3860567.00 5461334.00 1064599.00 4736435.00 9202906.00 8381597.00 6134595.00 4438200.00 8710819.00 5838201.00 13097562.00 G 2105888.00 8820562.00 8713085.00 1547575.00 7989437.00 7997838.00 6467069.00 8040383.00 2751604.00 8289383.00 2345829.00 5529099.00 H 31180.00 32087.00 32238.00 35524.00 34259.00 35090.00 35486.00 34938.00 35127.00 32124.00 30085.00 30840.00