Load time: 06/13/2013 11:12:33 AM Read time: 06/13/2013 11:12:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3063 Comment: 05282013(1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 80116960 cps Min counts: 560138 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 61305588.00 76437488.00 80116960.00 1215822.00 6348881.00 7155607.00 3875166.00 4444298.00 18056254.00 4476990.00 1206853.00 1120598.00 B 50168332.00 51692980.00 52367088.00 1185950.00 4391287.00 8952230.00 12319866.00 10894570.00 12551529.00 11358916.00 1206097.00 1120277.00 C 26733440.00 27247768.00 30456764.00 1188254.00 6100212.00 16239286.00 22124412.00 12464628.00 11211693.00 32406712.00 1207325.00 1198733.00 D 14990748.00 15660850.00 16171309.00 1197185.00 12073827.00 18593916.00 15284358.00 23562508.00 16360061.00 8351872.00 10125182.00 15260743.00 E 9713394.00 8927048.00 9773416.00 1180701.00 10362241.00 14064833.00 16593832.00 23582336.00 23997206.00 24113172.00 3273359.00 32287530.00 F 5695376.00 5519230.00 5815244.00 1184307.00 3663019.00 19628586.00 23487018.00 18945864.00 21573682.00 17163806.00 7581179.00 17785206.00 G 3781421.00 3957163.00 4148975.00 1176546.00 10880057.00 17178714.00 25741112.00 12810483.00 21952224.00 15583134.00 16441547.00 34361164.00 H 2578689.00 2544831.00 2634223.00 1154133.00 4563160.00 12967946.00 15083424.00 14819243.00 16606408.00 12325362.00 7513782.00 7539631.00