Load time: 01/23/2012 04:12:49 PM Read time: 01/23/2012 04:12:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2153 Comment: Standards, H1706 RESCAN, H1706 TILLING Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25564646 cps Min counts: 779062 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16401446.00 18486068.00 6817767.00 5784168.00 16955300.00 6274031.00 20739372.00 11665191.00 14324891.00 17742248.00 5488548.00 7102729.00 B 10167139.00 8991665.00 7424730.00 7493684.00 17523238.00 7419145.00 9126740.00 10179401.00 14725484.00 13411825.00 10145465.00 4967865.00 C 8797782.00 13180874.00 8276703.00 10032401.00 19987022.00 6768108.00 12565782.00 19936998.00 16267708.00 2894386.00 4363846.00 10946988.00 D 7327619.00 13080489.00 14688256.00 20095028.00 25564646.00 10268233.00 17803798.00 12523001.00 10358808.00 16983318.00 9160899.00 18572314.00 E 6448106.00 21527476.00 3682540.00 4624049.00 20546296.00 14271437.00 9672708.00 17410804.00 12419609.00 17298922.00 9065250.00 6782911.00 F 4671585.00 9200464.00 4858752.00 4966943.00 11768025.00 8905694.00 17004818.00 13727749.00 8014918.00 21871846.00 8862072.00 3193039.00 G 4489759.00 8068709.00 3146432.00 5167020.00 8909330.00 6762304.00 11104853.00 10753723.00 5296636.00 11747973.00 11423047.00 7166043.00 H 4044137.00 9603054.00 2886607.00 3657895.00 7170370.00 4845808.00 17321922.00 9037551.00 10570062.00 16392404.00 5743512.00 6252705.00