Load time: 01/23/2012 04:15:11 PM Read time: 01/23/2012 04:15:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2154 Comment: Standards, H1706 RESCAN, H1706 TILLING Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10123067 cps Min counts: 459838 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3421606.00 3088948.00 5856337.00 3439654.00 3906867.00 4896934.00 2947730.00 3124800.00 3015150.00 2449061.00 2276940.00 2614931.00 B 4564482.00 2037000.00 2682120.00 2923570.00 4726002.00 1880874.00 2457286.00 2617259.00 5027780.00 2521420.00 3429014.00 4473972.00 C 3718881.00 4767455.00 6932478.00 5008474.00 4850555.00 1494217.00 3905725.00 4550543.00 5085850.00 2532158.00 4150833.00 2188952.00 D 5066609.00 1939006.00 2691433.00 10123067.00 7158715.00 3844173.00 3951551.00 1792403.00 1255401.00 4181665.00 3567168.00 2802685.00 E 3795650.00 2402746.00 921845.00 3371665.00 3912498.00 2546084.00 2185004.00 2055111.00 2346573.00 5941125.00 1773739.00 3540140.00 F 3023536.00 3688343.00 2249857.00 3448454.00 5744893.00 3550214.00 3933318.00 1814244.00 3872467.00 2987720.00 4377226.00 4027076.00 G 2082545.00 4749020.00 2821417.00 4183892.00 7324773.00 3781056.00 4685045.00 4927267.00 2300930.00 3890484.00 919677.00 3708712.00 H 3377481.00 2708395.00 1349721.00 4086092.00 5605485.00 2408212.00 3928773.00 4132015.00 4043444.00 3550506.00 1600835.00 1267079.00