Load time: 01/23/2012 04:17:34 PM Read time: 01/23/2012 04:17:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2155 Comment: Standards, H1706 RESCAN, H1706 TILLING Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16398579 cps Min counts: 12952 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4188461.00 10037016.00 7338344.00 6948987.00 7703456.00 570545.00 7024152.00 31097.00 228906.00 670324.00 320355.00 111191.00 B 3923406.00 9345093.00 11262583.00 6491881.00 10632147.00 6914449.00 10024746.00 30508.00 168232.00 104382.00 544297.00 196115.00 C 4239572.00 8004707.00 9816526.00 3674682.00 7728308.00 951555.00 10544780.00 45248.00 147443.00 87092.00 220404.00 136811.00 D 10361205.00 5378956.00 16398579.00 11389614.00 14108077.00 9269054.00 8489891.00 30070.00 148527.00 99817.00 662469.00 323398.00 E 6111666.00 5901901.00 6691749.00 6572622.00 9204322.00 9441208.00 7976661.00 29918.00 113854.00 584658.00 901228.00 138409.00 F 5166561.00 4499299.00 6545228.00 4709110.00 10132178.00 12105320.00 9044234.00 29328.00 157124.00 137420.00 582185.00 89337.00 G 4146748.00 7101486.00 10985129.00 5792645.00 9190056.00 7283317.00 9354227.00 28891.00 343217.00 689591.00 323892.00 115927.00 H 28872.00 30013.00 30222.00 29671.00 27217.00 26418.00 27274.00 27560.00 28320.00 27978.00 25905.00 26837.00