Load time: 01/23/2012 04:10:23 PM Read time: 01/23/2012 04:10:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2152 Comment: Standards, H1706 RESCAN, H1706 TILLING Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 43796076 cps Min counts: 559000 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6050742.00 6484743.00 12775343.00 5653908.00 7537073.00 8751177.00 23216982.00 10839654.00 10724147.00 9571117.00 9275874.00 14326578.00 B 7543168.00 6517417.00 4557977.00 13704615.00 11601715.00 13981507.00 43796076.00 27780290.00 14936952.00 11271430.00 7920026.00 11421557.00 C 10625579.00 8305239.00 11594532.00 22122770.00 31238958.00 16733439.00 20754844.00 12650049.00 16630885.00 6779476.00 12345626.00 12187738.00 D 6747826.00 9022205.00 8246713.00 6971024.00 18139828.00 9709318.00 13927442.00 30058728.00 1308828.00 17349852.00 13983941.00 20039154.00 E 13810318.00 9472685.00 9346852.00 12236245.00 15697181.00 11070989.00 18458826.00 15593249.00 8240224.00 10546494.00 26895650.00 8819036.00 F 11765432.00 12736392.00 8148400.00 7375680.00 16055556.00 7825365.00 14775980.00 6385289.00 5450529.00 10200499.00 8779441.00 10212534.00 G 6797037.00 11111523.00 3723771.00 1118000.00 9876911.00 9341799.00 7599887.00 9634454.00 6619043.00 3399187.00 11978237.00 2628127.00 H 8360856.00 5944865.00 3729641.00 6551288.00 8230492.00 7225855.00 6282764.00 6383128.00 4338550.00 5898924.00 7213518.00 6223068.00